A Slow-Release Substrate Stimulates Groundwater Microbial Communities for Long-Term in Situ Cr(VI) Reduction
Abstract
Cr(VI) is a widespread environmental contaminant that is highly toxic and soluble. Previous work indicated that a one-time amendment of polylactate hydrogen-release compound (HRC) reduced groundwater Cr(VI) concentrations for >3.5 years at a contaminated aquifer; however, microbial communities responsible for Cr(VI) reduction are poorly understood. Here in this study, we hypothesized that HRC amendment would significantly change the composition and structure of groundwater microbial communities, and that the abundance of key functional genes involved in HRC degradation and electron acceptor reduction would increase long-term in response to this slowly degrading, complex substrate. To test these hypotheses, groundwater microbial communities were monitored after HRC amendment for >1 year using a comprehensive functional gene microarray. The results showed that the overall functional composition and structure of groundwater microbial communities underwent sequential shifts after HRC amendment. Particularly, the abundance of functional genes involved in acetate oxidation, denitrification, dissimilatory nitrate reduction, metal reduction, and sulfate reduction significantly increased. The overall community dynamics was significantly correlated with changes in groundwater concentrations of microbial biomass, acetate, NO3-, Cr(VI), Fe(II) and SO42-. Finally, our results suggest that HRC amendment primarily stimulated key functional processes associated with HRC degradation and reduction of multiple electron acceptors in the aquifermore »
- Authors:
-
- Univ. of Oklahoma, Norman, OK (United States). Inst. for Environmental Genomics, and Dept. of Microbiology and Plant Biology
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Earth Science Division
- Univ. of Oklahoma, Norman, OK (United States). Inst. for Environmental Genomics, and Dept. of Microbiology and Plant Biology; Chinese Academy of Sciences (CAS), Beijing (China). CAS Key Lab. of Environmental Biotechnology, Research Center for Eco-Environmental Sciences
- Montana State Univ., Bozeman, MT (United States). Center for Biofilm Engineering
- Univ. of Tennessee, Knoxville, TN (United States). Dept. of Civil and Environmental Engineering; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Physical Biosciences Division
- Univ. of Oklahoma, Norman, OK (United States). Inst. for Environmental Genomics, and Dept. of Microbiology and Plant Biology; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Earth Science Division
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES); USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1379163
- Grant/Contract Number:
- AC02-05CH11231; FG02-07ER64398
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Environmental Science and Technology
- Additional Journal Information:
- Journal Volume: 49; Journal Issue: 21; Journal ID: ISSN 0013-936X
- Publisher:
- American Chemical Society (ACS)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 54 ENVIRONMENTAL SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Citation Formats
Zhang, Ping, Van Nostrand, Joy D., He, Zhili, Chakraborty, Romy, Deng, Ye, Curtis, Daniel, Fields, Matthew W., Hazen, Terry C., Arkin, Adam P., and Zhou, Jizhong. A Slow-Release Substrate Stimulates Groundwater Microbial Communities for Long-Term in Situ Cr(VI) Reduction. United States: N. p., 2015.
Web. doi:10.1021/acs.est.5b00024.
Zhang, Ping, Van Nostrand, Joy D., He, Zhili, Chakraborty, Romy, Deng, Ye, Curtis, Daniel, Fields, Matthew W., Hazen, Terry C., Arkin, Adam P., & Zhou, Jizhong. A Slow-Release Substrate Stimulates Groundwater Microbial Communities for Long-Term in Situ Cr(VI) Reduction. United States. https://doi.org/10.1021/acs.est.5b00024
Zhang, Ping, Van Nostrand, Joy D., He, Zhili, Chakraborty, Romy, Deng, Ye, Curtis, Daniel, Fields, Matthew W., Hazen, Terry C., Arkin, Adam P., and Zhou, Jizhong. Thu .
"A Slow-Release Substrate Stimulates Groundwater Microbial Communities for Long-Term in Situ Cr(VI) Reduction". United States. https://doi.org/10.1021/acs.est.5b00024. https://www.osti.gov/servlets/purl/1379163.
@article{osti_1379163,
title = {A Slow-Release Substrate Stimulates Groundwater Microbial Communities for Long-Term in Situ Cr(VI) Reduction},
author = {Zhang, Ping and Van Nostrand, Joy D. and He, Zhili and Chakraborty, Romy and Deng, Ye and Curtis, Daniel and Fields, Matthew W. and Hazen, Terry C. and Arkin, Adam P. and Zhou, Jizhong},
abstractNote = {Cr(VI) is a widespread environmental contaminant that is highly toxic and soluble. Previous work indicated that a one-time amendment of polylactate hydrogen-release compound (HRC) reduced groundwater Cr(VI) concentrations for >3.5 years at a contaminated aquifer; however, microbial communities responsible for Cr(VI) reduction are poorly understood. Here in this study, we hypothesized that HRC amendment would significantly change the composition and structure of groundwater microbial communities, and that the abundance of key functional genes involved in HRC degradation and electron acceptor reduction would increase long-term in response to this slowly degrading, complex substrate. To test these hypotheses, groundwater microbial communities were monitored after HRC amendment for >1 year using a comprehensive functional gene microarray. The results showed that the overall functional composition and structure of groundwater microbial communities underwent sequential shifts after HRC amendment. Particularly, the abundance of functional genes involved in acetate oxidation, denitrification, dissimilatory nitrate reduction, metal reduction, and sulfate reduction significantly increased. The overall community dynamics was significantly correlated with changes in groundwater concentrations of microbial biomass, acetate, NO3-, Cr(VI), Fe(II) and SO42-. Finally, our results suggest that HRC amendment primarily stimulated key functional processes associated with HRC degradation and reduction of multiple electron acceptors in the aquifer toward long-term Cr(VI) reduction.},
doi = {10.1021/acs.est.5b00024},
journal = {Environmental Science and Technology},
number = 21,
volume = 49,
place = {United States},
year = {Thu Apr 02 00:00:00 EDT 2015},
month = {Thu Apr 02 00:00:00 EDT 2015}
}
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