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Title: Reduction of ethanol yield from switchgrass infected with rust caused by Puccinia emaculata

Abstract

Switchgrass (Panicum virgatum) is an important biofuel crop candidate thought to have low disease susceptibility. As switchgrass production becomes more prevalent, monoculture and production fields in close proximity to one another may increase the spread and severity of diseases such as switchgrass rust caused by the pathogen Puccinia emaculata. The objective of this research was to examine the impact of rust on ethanol yield in switchgrass. In 2010 and 2012, naturally infected leaves from field-grown 'Alamo' and 'Kanlow' in Knoxville, TN (2010, 2012) and Crossville, TN (2012) were visually categorized as exhibiting low, medium, or high disease based on the degree of chlorosis and sporulation. P. emaculata was isolated from each disease range to confirm infection. Samples from 2010 were acid/heat pretreated and subjected to two runs of simultaneous saccharification and fermentation (SSF) with Saccharomyces cerevisiae D5A to measure ethanol yield. Near-infrared spectroscopy (NIRS) was used to estimate ethanol yield for 2012 samples. SSF and NIRS data were analyzed separately using ANOVA. Disease level effects were significant within both models (P < 0.05) and both models explained a large amount of variation in ETOH (SSF: R2 = 0.99, NIRS: R2 = 0.99). In the SSF dataset, ethanol was reduced bymore » 35 % in samples exhibiting medium disease symptoms and by 55 % in samples exhibiting high disease symptoms. In the NIRS dataset, estimated ethanol was reduced by 10 % in samples exhibiting medium disease symptoms and by 21 % in samples exhibiting high disease symptoms. Lastly, results indicate that switchgrass rust will likely have a negative impact on ethanol yield in switchgrass grown as a biofuel crop.« less

Authors:
 [1];  [1];  [2];  [3];  [1];  [4];  [5];  [6]
+ Show Author Affiliations
  1. Univ. of Tennessee, Knoxville, TN (United States)
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); White Cliff Biosystems, Rockwood, TN (United States)
  3. Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  4. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Center for Renewable Carbon, Knoxville, TN (United States)
  5. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  6. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Genomatica Inc., San Diego, CA (United States)
Publication Date:
Research Org.:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1327741
Alternate Identifier(s):
OSTI ID: 1348317
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Accepted Manuscript
Journal Name:
BioEnergy Research
Additional Journal Information:
Journal Volume: 9; Journal Issue: 1; Journal ID: ISSN 1939-1234
Publisher:
Springer
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 60 APPLIED LIFE SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; NIRS; rust; Puccinia emaculata; switchgrass; ethanol; SSF; Panicum virgatum

Citation Formats

Sykes, Virginia R., Allen, Fred L., Mielenz, Jonathan R., Stewart, Jr., C. Neal, Windham, Mark T., Hamilton, Choo Y., Rodriguez, Miguel, and Yee, Kelsey L. Reduction of ethanol yield from switchgrass infected with rust caused by Puccinia emaculata. United States: N. p., 2015. Web. doi:10.1007/s12155-015-9680-4.
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Sykes, Virginia R., Allen, Fred L., Mielenz, Jonathan R., Stewart, Jr., C. Neal, Windham, Mark T., Hamilton, Choo Y., Rodriguez, Miguel, & Yee, Kelsey L. Reduction of ethanol yield from switchgrass infected with rust caused by Puccinia emaculata. United States. https://doi.org/10.1007/s12155-015-9680-4
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Sykes, Virginia R., Allen, Fred L., Mielenz, Jonathan R., Stewart, Jr., C. Neal, Windham, Mark T., Hamilton, Choo Y., Rodriguez, Miguel, and Yee, Kelsey L. Fri . "Reduction of ethanol yield from switchgrass infected with rust caused by Puccinia emaculata". United States. https://doi.org/10.1007/s12155-015-9680-4. https://www.osti.gov/servlets/purl/1327741.
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@article{osti_1327741,
title = {Reduction of ethanol yield from switchgrass infected with rust caused by Puccinia emaculata},
author = {Sykes, Virginia R. and Allen, Fred L. and Mielenz, Jonathan R. and Stewart, Jr., C. Neal and Windham, Mark T. and Hamilton, Choo Y. and Rodriguez, Miguel and Yee, Kelsey L.},
abstractNote = {Switchgrass (Panicum virgatum) is an important biofuel crop candidate thought to have low disease susceptibility. As switchgrass production becomes more prevalent, monoculture and production fields in close proximity to one another may increase the spread and severity of diseases such as switchgrass rust caused by the pathogen Puccinia emaculata. The objective of this research was to examine the impact of rust on ethanol yield in switchgrass. In 2010 and 2012, naturally infected leaves from field-grown 'Alamo' and 'Kanlow' in Knoxville, TN (2010, 2012) and Crossville, TN (2012) were visually categorized as exhibiting low, medium, or high disease based on the degree of chlorosis and sporulation. P. emaculata was isolated from each disease range to confirm infection. Samples from 2010 were acid/heat pretreated and subjected to two runs of simultaneous saccharification and fermentation (SSF) with Saccharomyces cerevisiae D5A to measure ethanol yield. Near-infrared spectroscopy (NIRS) was used to estimate ethanol yield for 2012 samples. SSF and NIRS data were analyzed separately using ANOVA. Disease level effects were significant within both models (P < 0.05) and both models explained a large amount of variation in ETOH (SSF: R2 = 0.99, NIRS: R2 = 0.99). In the SSF dataset, ethanol was reduced by 35 % in samples exhibiting medium disease symptoms and by 55 % in samples exhibiting high disease symptoms. In the NIRS dataset, estimated ethanol was reduced by 10 % in samples exhibiting medium disease symptoms and by 21 % in samples exhibiting high disease symptoms. Lastly, results indicate that switchgrass rust will likely have a negative impact on ethanol yield in switchgrass grown as a biofuel crop.},
doi = {10.1007/s12155-015-9680-4},
journal = {BioEnergy Research},
number = 1,
volume = 9,
place = {United States},
year = {Fri Oct 16 00:00:00 EDT 2015},
month = {Fri Oct 16 00:00:00 EDT 2015}
}
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https://doi.org/10.1007/s12155-015-9680-4
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Works referenced in this record:

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    Works referencing / citing this record:

    QTL × environment interactions underlie adaptive divergence in switchgrass across a large latitudinal gradient
    journal, June 2019

    • Lowry, David B.; Lovell, John T.; Zhang, Li
    • Proceedings of the National Academy of Sciences, Vol. 116, Issue 26
    • DOI: 10.1073/pnas.1821543116

    Rust fungi on Panicum
    journal, January 2017

    The Genetic Basis of Upland/Lowland Ecotype Divergence in Switchgrass ( Panicum virgatum)
    journal, September 2016

    • Milano, Elizabeth R.; Lowry, David B.; Juenger, Thomas E.
    • G3&#58; Genes|Genomes|Genetics, Vol. 6, Issue 11
    • DOI: 10.1534/g3.116.032763

    Transcriptome divergence during leaf development in two contrasting switchgrass (Panicum virgatum L.) cultivars
    journal, September 2019

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