DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Succinic acid production from lignocellulosic hydrolysate by Basfia succiniciproducens

Abstract

The production of chemicals alongside fuels will be essential to enhance the feasibility of lignocellulosic biorefineries. Succinic acid (SA), a naturally occurring C4-diacid, is a primary intermediate of the tricarboxylic acid cycle and a promising building block chemical that has received significant industrial attention. Basfia succiniciproducens is a relatively unexplored SA-producing bacterium with advantageous features such as broad substrate utilization, genetic tractability, and facultative anaerobic metabolism. Here B. succiniciproducens is evaluated in high xylose-content hydrolysates from corn stover and different synthetic media in batch fermentation. SA titers in hydrolysate at an initial sugar concentration of 60 g/L reached up to 30 g/L, with metabolic yields of 0.69 g/g, and an overall productivity of 0.43 g/L/h. These results demonstrate that B. succiniciproducens may be an attractive platform organism for bio-SA production from biomass hydrolysates.

Authors:
 [1];  [1];  [1];  [1];  [1];  [1];  [1];  [1]
  1. National Renewable Energy Lab. (NREL), Golden, CO (United States). National Bioenergy Center
Publication Date:
Research Org.:
National Renewable Energy Laboratory (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office (BETO)
OSTI Identifier:
1254113
Alternate Identifier(s):
OSTI ID: 1326455
Report Number(s):
NREL/JA-5100-66481
Journal ID: ISSN 0960-8524
Grant/Contract Number:  
AC36-08GO28308
Resource Type:
Accepted Manuscript
Journal Name:
Bioresource Technology
Additional Journal Information:
Journal Volume: 214; Journal Issue: C; Journal ID: ISSN 0960-8524
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; biorefinery; biochemical; corn stover; xylose; Actinobacillus succinogenes

Citation Formats

Salvachúa, Davinia, Smith, Holly, St. John, Peter C., Mohagheghi, Ali, Peterson, Darren J., Black, Brenna A., Dowe, Nancy, and Beckham, Gregg T. Succinic acid production from lignocellulosic hydrolysate by Basfia succiniciproducens. United States: N. p., 2016. Web. doi:10.1016/j.biortech.2016.05.018.
Salvachúa, Davinia, Smith, Holly, St. John, Peter C., Mohagheghi, Ali, Peterson, Darren J., Black, Brenna A., Dowe, Nancy, & Beckham, Gregg T. Succinic acid production from lignocellulosic hydrolysate by Basfia succiniciproducens. United States. https://doi.org/10.1016/j.biortech.2016.05.018
Salvachúa, Davinia, Smith, Holly, St. John, Peter C., Mohagheghi, Ali, Peterson, Darren J., Black, Brenna A., Dowe, Nancy, and Beckham, Gregg T. Mon . "Succinic acid production from lignocellulosic hydrolysate by Basfia succiniciproducens". United States. https://doi.org/10.1016/j.biortech.2016.05.018. https://www.osti.gov/servlets/purl/1254113.
@article{osti_1254113,
title = {Succinic acid production from lignocellulosic hydrolysate by Basfia succiniciproducens},
author = {Salvachúa, Davinia and Smith, Holly and St. John, Peter C. and Mohagheghi, Ali and Peterson, Darren J. and Black, Brenna A. and Dowe, Nancy and Beckham, Gregg T.},
abstractNote = {The production of chemicals alongside fuels will be essential to enhance the feasibility of lignocellulosic biorefineries. Succinic acid (SA), a naturally occurring C4-diacid, is a primary intermediate of the tricarboxylic acid cycle and a promising building block chemical that has received significant industrial attention. Basfia succiniciproducens is a relatively unexplored SA-producing bacterium with advantageous features such as broad substrate utilization, genetic tractability, and facultative anaerobic metabolism. Here B. succiniciproducens is evaluated in high xylose-content hydrolysates from corn stover and different synthetic media in batch fermentation. SA titers in hydrolysate at an initial sugar concentration of 60 g/L reached up to 30 g/L, with metabolic yields of 0.69 g/g, and an overall productivity of 0.43 g/L/h. These results demonstrate that B. succiniciproducens may be an attractive platform organism for bio-SA production from biomass hydrolysates.},
doi = {10.1016/j.biortech.2016.05.018},
journal = {Bioresource Technology},
number = C,
volume = 214,
place = {United States},
year = {Mon May 09 00:00:00 EDT 2016},
month = {Mon May 09 00:00:00 EDT 2016}
}

Journal Article:

Citation Metrics:
Cited by: 52 works
Citation information provided by
Web of Science

Save / Share:

Works referenced in this record:

Recent advances in production of succinic acid from lignocellulosic biomass
journal, November 2013

  • Akhtar, Junaid; Idris, Ani; Abd. Aziz, Ramlan
  • Applied Microbiology and Biotechnology, Vol. 98, Issue 3
  • DOI: 10.1007/s00253-013-5319-6

Microbial succinic acid production: Natural versus metabolic engineered producers
journal, July 2010


Systems-wide analysis and engineering of metabolic pathway fluxes in bio-succinate producing Basfia succiniciproducens: Systems-Wide Analysis and Engineering
journal, July 2013

  • Becker, Judith; Reinefeld, Jasper; Stellmacher, René
  • Biotechnology and Bioengineering, Vol. 110, Issue 11
  • DOI: 10.1002/bit.24963

The Techno-Economic Basis for Coproduct Manufacturing To Enable Hydrocarbon Fuel Production from Lignocellulosic Biomass
journal, May 2016


Continuous succinic acid production by Actinobacillus succinogenes in a biofilm reactor: Steady-state metabolic flux variation
journal, April 2014


Continuous succinic acid production by Actinobacillus succinogenes on xylose-enriched hydrolysate
journal, November 2015

  • Bradfield, Michael F. A.; Mohagheghi, Ali; Salvachúa, Davinia
  • Biotechnology for Biofuels, Vol. 8, Issue 1
  • DOI: 10.1186/s13068-015-0363-3

Biotechnological production of succinic acid: current state and perspectives
journal, February 2012

  • Cheng, Ke-Ke; Zhao, Xue-Bing; Zeng, Jing
  • Biofuels, Bioproducts and Biorefining, Vol. 6, Issue 3
  • DOI: 10.1002/bbb.1327

Deconstruction of Lignocellulosic Biomass to Fuels and Chemicals
journal, July 2011


Succinic acid production derived from carbohydrates: An energy and greenhouse gas assessment of a platform chemical toward a bio-based economy
journal, August 2013

  • Cok, Benjamin; Tsiropoulos, Ioannis; Roes, Alexander L.
  • Biofuels, Bioproducts and Biorefining, Vol. 8, Issue 1
  • DOI: 10.1002/bbb.1427

Kinetic study of succinic acid production by Actinobacillus succinogenes ZT-130
journal, October 2008

  • Corona-González, Rosa Isela; Bories, Andre; González-Álvarez, Víctor
  • Process Biochemistry, Vol. 43, Issue 10
  • DOI: 10.1016/j.procbio.2008.05.011

Succinic Acid Production with Actinobacillus succinogenes ZT-130 in the Presence of Succinic Acid
journal, September 2009

  • Corona-Gonzalez, Rosa Isela; Bories, Andre; González-Álvarez, Víctor
  • Current Microbiology, Vol. 60, Issue 1
  • DOI: 10.1007/s00284-009-9504-x

Inhibition of growth of Zymomonas mobilis by model compounds found in lignocellulosic hydrolysates
journal, January 2013

  • Franden, Mary Ann; Pilath, Heidi M.; Mohagheghi, Ali
  • Biotechnology for Biofuels, Vol. 6, Issue 1
  • DOI: 10.1186/1754-6834-6-99

Batch and continuous fermentation of succinic acid from wood hydrolysate by Mannheimia succiniciproducens MBEL55E
journal, December 2004


Basfia succiniciproducens gen. nov., sp. nov., a new member of the family Pasteurellaceae isolated from bovine rumen
journal, July 2009

  • Kuhnert, P.; Scholten, E.; Haefner, S.
  • INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 60, Issue 1
  • DOI: 10.1099/ijs.0.011809-0

Biological conversion of wood hydrolysate to succinic acid by Anaerobiospirillum succiniciproducens
journal, January 2003

  • Lee, P. C.; Lee, S. Y.; Hong, S. H.
  • Biotechnology Letters, Vol. 25, Issue 2, p. 111-114
  • DOI: 10.1023/A:1021907116361

A genomic perspective on the potential of Actinobacillus succinogenes for industrial succinate production
journal, January 2010

  • McKinlay, James B.; Laivenieks, Maris; Schindler, Bryan D.
  • BMC Genomics, Vol. 11, Issue 1
  • DOI: 10.1186/1471-2164-11-680

An efficient succinic acid production process in a metabolically engineered Corynebacterium glutamicum strain
journal, September 2008

  • Okino, Shohei; Noburyu, Ryoji; Suda, Masako
  • Applied Microbiology and Biotechnology, Vol. 81, Issue 3
  • DOI: 10.1007/s00253-008-1668-y

Fermentation of lignocellulosic hydrolysates. I: inhibition and detoxification
journal, August 2000


Succinic acid production on xylose-enriched biorefinery streams by Actinobacillus succinogenes in batch fermentation
journal, February 2016

  • Salvachúa, Davinia; Mohagheghi, Ali; Smith, Holly
  • Biotechnology for Biofuels, Vol. 9, Issue 1
  • DOI: 10.1186/s13068-016-0425-1

Succinic acid production by a newly isolated bacterium
journal, July 2008


Continuous cultivation approach for fermentative succinic acid production from crude glycerol by Basfia succiniciproducens DD1
journal, August 2009


Modeling of batch fermentation kinetics for succinic acid production by Mannheimia succiniciproducens
journal, May 2008

  • Song, Hyohak; Jang, Seh Hee; Park, Jong Myoung
  • Biochemical Engineering Journal, Vol. 40, Issue 1
  • DOI: 10.1016/j.bej.2007.11.021

Development of chemically defined medium for Mannheimia succiniciproducens based on its genome sequence
journal, May 2008

  • Song, Hyohak; Kim, Tae Yong; Choi, Bo-Kyeong
  • Applied Microbiology and Biotechnology, Vol. 79, Issue 2
  • DOI: 10.1007/s00253-008-1425-2

Insight into Biomass as a Renewable Carbon Source for the Production of Succinic Acid and the Factors Affecting the Metabolic Flux toward Higher Succinate Yield
journal, October 2014

  • Tan, Jian Ping; Md. Jahim, Jamaliah; Wu, Ta Yeong
  • Industrial & Engineering Chemistry Research, Vol. 53, Issue 42
  • DOI: 10.1021/ie502178j

Large-scale computation of elementary flux modes with bit pattern trees
journal, August 2008


Continuous succinic acid fermentation by Actinobacillus succinogenes
journal, April 2013


Succinic Acid Production from Corn Cob Hydrolysates by Genetically Engineered Corynebacterium glutamicum
journal, October 2013

  • Wang, Chen; Zhang, Hengli; Cai, Heng
  • Applied Biochemistry and Biotechnology, Vol. 172, Issue 1
  • DOI: 10.1007/s12010-013-0539-x

Efficient production of succinic acid from corn stalk hydrolysates by a recombinant Escherichia coli with ptsG mutation
journal, January 2011


Effects of lignocellulose-derived inhibitors on growth and succinic acid accumulation by Corynebacterium glutamicum
journal, August 2015

  • Xu, Hong-Tao; Wang, Chen; Zhou, Zhi-Hui
  • Biotechnology and Bioprocess Engineering, Vol. 20, Issue 4
  • DOI: 10.1007/s12257-015-0201-2

Series fermentation production of ornithine and succinic acid from cane molasses by Corynebacterium glutamicum
journal, July 2015


Enhanced succinic acid production by Actinobacillus succinogenes after genome shuffling
journal, May 2013

  • Zheng, Pu; Zhang, Kunkun; Yan, Qiang
  • Journal of Industrial Microbiology & Biotechnology, Vol. 40, Issue 8
  • DOI: 10.1007/s10295-013-1283-5

Works referencing / citing this record:

Bioreactors for succinic acid production processes
journal, March 2019

  • Ferone, Mariateresa; Raganati, Francesca; Olivieri, Giuseppe
  • Critical Reviews in Biotechnology, Vol. 39, Issue 4
  • DOI: 10.1080/07388551.2019.1592105

Homogeneous solid dispersion (HSD) system for rapid and stable production of succinic acid from lignocellulosic hydrolysate
journal, September 2018

  • Luthfi, Abdullah Amru Indera; Tan, Jian Ping; Harun, Shuhaida
  • Bioprocess and Biosystems Engineering, Vol. 42, Issue 1
  • DOI: 10.1007/s00449-018-2019-8

Biotechnological route for sustainable succinate production utilizing oil palm frond and kenaf as potential carbon sources
journal, March 2017

  • Luthfi, Abdullah Amru Indera; Manaf, Shareena Fairuz Abdul; Illias, Rosli Md
  • Applied Microbiology and Biotechnology, Vol. 101, Issue 8
  • DOI: 10.1007/s00253-017-8210-z

Bio-Based Succinate Production from Arundo donax Hydrolysate with the New Natural Succinic Acid-Producing Strain Basfia succiniciproducens BPP7
journal, January 2017

  • Ventorino, Valeria; Robertiello, Alessandro; Cimini, Donatella
  • BioEnergy Research, Vol. 10, Issue 2
  • DOI: 10.1007/s12155-017-9814-y

Metabolic engineering of Corynebacterium glutamicum for efficient production of succinate from lignocellulosic hydrolysate
journal, April 2018


Improved production of succinic acid from Basfia succiniciproducens growing on A. donax and process evaluation through material flow analysis
journal, February 2019

  • Cimini, Donatella; Zaccariello, Lucio; D’Ambrosio, Sergio
  • Biotechnology for Biofuels, Vol. 12, Issue 1
  • DOI: 10.1186/s13068-019-1362-6

Continuous Succinic Acid Fermentation by Actinobacillus Succinogenes: Assessment of Growth and Succinic Acid Production Kinetics
journal, August 2018

  • Ferone, Mariateresa; Raganati, Francesca; Olivieri, Giuseppe
  • Applied Biochemistry and Biotechnology, Vol. 187, Issue 3
  • DOI: 10.1007/s12010-018-2846-8

Propionic acid production from corn stover hydrolysate by Propionibacterium acidipropionici
journal, August 2017

  • Wang, Xiaoqing; Salvachúa, Davinia; Sànchez i. Nogué, Violeta
  • Biotechnology for Biofuels, Vol. 10, Issue 1
  • DOI: 10.1186/s13068-017-0884-z

Improved production of succinic acid from Basfia succiniciproducens growing on A. donax and process evaluation through material flow analysis
journal, February 2019

  • Cimini, Donatella; Zaccariello, Lucio; D’Ambrosio, Sergio
  • Biotechnology for Biofuels, Vol. 12, Issue 1
  • DOI: 10.1186/s13068-019-1362-6