Metabolic engineering of Zymomonas mobilis for 2,3-butanediol production from lignocellulosic biomass sugars
Abstract
To develop pathways for advanced biofuel production, and to understand the impact of host metabolism and environmental conditions on heterologous pathway engineering for economic advanced biofuels production from biomass, we seek to redirect the carbon flow of the model ethanologen Zymomonas mobilis to produce desirable hydrocarbon intermediate 2,3-butanediol (2,3-BDO). 2,3-BDO is a bulk chemical building block, and can be upgraded in high yields to gasoline, diesel, and jet fuel. 2,3-BDO biosynthesis pathways from various bacterial species were examined, which include three genes encoding acetolactate synthase, acetolactate decarboxylase, and butanediol dehydrogenase. Bioinformatics analysis was carried out to pinpoint potential bottlenecks for high 2,3-BDO production. Different combinations of 2,3-BDO biosynthesis metabolic pathways using genes from different bacterial species have been constructed. Our results demonstrated that carbon flux can be deviated from ethanol production into 2,3-BDO biosynthesis, and all three heterologous genes are essential to efficiently redirect pyruvate from ethanol production for high 2,3-BDO production in Z. mobilis. The down-selection of best gene combinations up to now enabled Z. mobilis to reach the 2,3-BDO production of more than 10 g/L from glucose and xylose, as well as mixed C6/C5 sugar streams derived from the deacetylation and mechanical refining process. In conclusion, this studymore »
- Publication Date:
- Research Org.:
- National Renewable Energy Laboratory (NREL), Golden, CO (United States)
- Sponsoring Org.:
- USDOE Office of Energy Efficiency and Renewable Energy (EERE), Office of Sustainable Transportation. Bioenergy Technologies Office (BETO); USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office (BETO)
- OSTI Identifier:
- 1618645
- Alternate Identifier(s):
- OSTI ID: 1325930
- Report Number(s):
- NREL/JA-5100-67146
Journal ID: ISSN 1754-6834; 189; PII: 606
- Grant/Contract Number:
- AC36-08GO28308
- Resource Type:
- Published Article
- Journal Name:
- Biotechnology for Biofuels
- Additional Journal Information:
- Journal Name: Biotechnology for Biofuels Journal Volume: 9 Journal Issue: 1; Journal ID: ISSN 1754-6834
- Publisher:
- Springer Science + Business Media
- Country of Publication:
- Netherlands
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; Zymomonas mobilis; 2,3-Butanediol; metabolic engineering; omics; redox balance; advanced biofuel; fermentation; respiration chain
Citation Formats
Yang, Shihui, Mohagheghi, Ali, Franden, Mary Ann, Chou, Yat-Chen, Chen, Xiaowen, Dowe, Nancy, Himmel, Michael E., and Zhang, Min. Metabolic engineering of Zymomonas mobilis for 2,3-butanediol production from lignocellulosic biomass sugars. Netherlands: N. p., 2016.
Web. doi:10.1186/s13068-016-0606-y.
Yang, Shihui, Mohagheghi, Ali, Franden, Mary Ann, Chou, Yat-Chen, Chen, Xiaowen, Dowe, Nancy, Himmel, Michael E., & Zhang, Min. Metabolic engineering of Zymomonas mobilis for 2,3-butanediol production from lignocellulosic biomass sugars. Netherlands. https://doi.org/10.1186/s13068-016-0606-y
Yang, Shihui, Mohagheghi, Ali, Franden, Mary Ann, Chou, Yat-Chen, Chen, Xiaowen, Dowe, Nancy, Himmel, Michael E., and Zhang, Min. Fri .
"Metabolic engineering of Zymomonas mobilis for 2,3-butanediol production from lignocellulosic biomass sugars". Netherlands. https://doi.org/10.1186/s13068-016-0606-y.
@article{osti_1618645,
title = {Metabolic engineering of Zymomonas mobilis for 2,3-butanediol production from lignocellulosic biomass sugars},
author = {Yang, Shihui and Mohagheghi, Ali and Franden, Mary Ann and Chou, Yat-Chen and Chen, Xiaowen and Dowe, Nancy and Himmel, Michael E. and Zhang, Min},
abstractNote = {To develop pathways for advanced biofuel production, and to understand the impact of host metabolism and environmental conditions on heterologous pathway engineering for economic advanced biofuels production from biomass, we seek to redirect the carbon flow of the model ethanologen Zymomonas mobilis to produce desirable hydrocarbon intermediate 2,3-butanediol (2,3-BDO). 2,3-BDO is a bulk chemical building block, and can be upgraded in high yields to gasoline, diesel, and jet fuel. 2,3-BDO biosynthesis pathways from various bacterial species were examined, which include three genes encoding acetolactate synthase, acetolactate decarboxylase, and butanediol dehydrogenase. Bioinformatics analysis was carried out to pinpoint potential bottlenecks for high 2,3-BDO production. Different combinations of 2,3-BDO biosynthesis metabolic pathways using genes from different bacterial species have been constructed. Our results demonstrated that carbon flux can be deviated from ethanol production into 2,3-BDO biosynthesis, and all three heterologous genes are essential to efficiently redirect pyruvate from ethanol production for high 2,3-BDO production in Z. mobilis. The down-selection of best gene combinations up to now enabled Z. mobilis to reach the 2,3-BDO production of more than 10 g/L from glucose and xylose, as well as mixed C6/C5 sugar streams derived from the deacetylation and mechanical refining process. In conclusion, this study confirms the value of integrating bioinformatics analysis and systems biology data during metabolic engineering endeavors, provides guidance for value-added chemical production in Z. mobilis, and reveals the interactions between host metabolism, oxygen levels, and a heterologous 2,3-BDO biosynthesis pathway. Taken together, this work provides guidance for future metabolic engineering efforts aimed at boosting 2,3-BDO titer anaerobically.},
doi = {10.1186/s13068-016-0606-y},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 9,
place = {Netherlands},
year = {Fri Sep 02 00:00:00 EDT 2016},
month = {Fri Sep 02 00:00:00 EDT 2016}
}
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https://doi.org/10.1186/s13068-016-0606-y
https://doi.org/10.1186/s13068-016-0606-y
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Cited by: 98 works
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Figures / Tables:
Fig. 1: The impact of meso‑2,3‑BDO and ethanol supplementation on growth of Z. mobilis in RMG medium at 30 °C using Bioscreen C. Relative growth rate (%) is the percentage of growth rate with chemical supplementation compared to control without
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