Title: Engineering E. coli–E. coli cocultures for production of muconic acid from glycerol

Abstract

cis, cis-Muconic acid is an important chemical that can be biosynthesized from simple substrates in engineered microorganisms. Recently, it has been shown that engineering microbial cocultures is an emerging and promising approach for biochemical production. In this study, we aim to explore the potential of the E. coli–E. coli coculture system to use a single renewable carbon source, glycerol, for the production of value-added product cis, cis-muconic acid. As a result, two coculture engineering strategies were investigated. In the first strategy, an E. coli strain containing the complete biosynthesis pathway was co-cultivated with another E. coli strain containing only a heterologous intermediate-to-product biosynthetic pathway. In the second strategy, the upstream and downstream pathways were accommodated in two separate E. coli strains, each of which was dedicated to one portion of the biosynthesis process. Compared with the monoculture approach, both coculture engineering strategies improved the production significantly. Using a batch bioreactor, the engineered coculture achieved a 2 g/L muconic acid production with a yield of 0.1 g/g. In conclusion, our results demonstrate that coculture engineering is a viable option for producing muconic acid from glycerol. Moreover, microbial coculture systems are shown to have the potential for converting single carbon source tomore » value-added products.« less

Authors:

Zhang, Haoran ;

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Li, Zhengjun; Pereira, Brian; Stephanopoulos, Gregory

Publication Date:

Tue Sep 15 00:00:00 EDT 2015

Research Org.:

Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1618794

Alternate Identifier(s):

OSTI ID: 1324979

Grant/Contract Number:  

SC0006698

Resource Type:

Published Article

Journal Name:

Microbial Cell Factories

Additional Journal Information:

Journal Name: Microbial Cell Factories Journal Volume: 14 Journal Issue: 1; Journal ID: ISSN 1475-2859

Publisher:

Springer Science + Business Media

Country of Publication:

United Kingdom

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES; metabolic engineering; E. coli; coculture; muconic acid; glycerol