skip to main content

DOE PAGESDOE PAGES

This content will become publicly available on January 8, 2017

Title: Three-dimensional imaging of cholesterol and sphingolipids within a Madin-Darby canine kidney cell

Metabolic stable isotope incorporation and secondary ion mass spectrometry(SIMS) depth profiling performed on a Cameca NanoSIMS 50 were used to image the 18O-cholesterol and 15N-sphingolipid distributions within a portion of a Madin-Darby canine kidney (MDCK) cell. Three-dimensional representations of the component-specific isotope distributions show clearly defined regions of 18O-cholesterol and 15N-sphingolipid enrichment that seem to be separate subcellular compartments. Furthermore, the low levels of nitrogen-containing secondary ions detected at the 18O-enriched regions suggest that these 18O-cholesterol-rich structures may be lipiddroplets, which have a core consisting of cholesterol esters and triacylglycerides.
Authors:
 [1] ;  [2] ;  [1]
  1. Univ. of Illinois, Urbana, IL (United States). Dept. of Chemical and Biomolecular Engineering
  2. Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States). Glenn T. Seaborg Institute
Publication Date:
OSTI Identifier:
1305902
Report Number(s):
LLNL-JRNL--680575
Journal ID: ISSN 1934-8630; BJIOBN
Grant/Contract Number:
AC52-07NA27344
Type:
Accepted Manuscript
Journal Name:
Biointerphases
Additional Journal Information:
Journal Volume: 11; Journal Issue: 2; Journal ID: ISSN 1934-8630
Publisher:
AIP
Research Org:
Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY cell membranes; secondary ion mass spectroscopy; lipids; acids; image analysis