skip to main content

DOE PAGESDOE PAGES

Title: A genetic screen identifies a requirement for cysteine-rich–receptor-like kinases in rice NH1 (OsNPR1)-mediated immunity

Systemic acquired resistance, mediated by the Arabidopsis NPR1 gene and the rice NH1 gene, confers broad-spectrum immunity to diverse pathogens. NPR1 and NH1 interact with TGA transcription factors to activate downstream defense genes. Despite the importance of this defense response, the signaling components downstream of NPR1/NH1 and TGA proteins are poorly defined. Here we report the identification of a rice mutant, snim1, which suppresses NH1-mediated immunity and demonstrate that two genes encoding previously uncharacterized cysteine-rich-receptor-like kinases (CRK6 and CRK10), complement the snim1 mutant phenotype. Silencing of CRK6 and CRK10 genes individually in the parental genetic background recreates the snim1 phenotype. We identified a rice mutant in the Kitaake genetic background with a frameshift mutation in crk10; this mutant also displays a compromised immune response highlighting the important role of crk10. We also show that elevated levels of NH1 expression lead to enhanced CRK10 expression and that the rice TGA2.1 protein binds to the CRK10 promoter. Furthermore, these experiments demonstrate a requirement for CRKs in NH1-mediated immunity and establish a molecular link between NH1 and induction of CRK10 expression.
Authors:
 [1] ;  [2] ;  [3] ;  [4] ;  [1] ;  [1] ;  [5] ;  [1] ;  [6] ;  [1] ;  [7]
  1. Univ. of California, Davis, CA (United States). Dept. of Plant Pathology and the Genome Center; Joint Bioenergy Inst., Emeryville, CA (United States)
  2. Univ. of California, Davis, CA (United States). Dept. of Plant Pathology and the Genome Center; Joint Bioenergy Inst., Emeryville, CA (United States); Jiangsu Academy of Agricultural Sciences, Nanjing (China). Inst. of Plant Protection
  3. Univ. of California, Davis, CA (United States). Dept. of Plant Pathology and the Genome Center; Joint Bioenergy Inst., Emeryville, CA (United States); Donald Danforth Center, St. Louis, MO (United States)
  4. Univ. of California, Davis, CA (United States). Dept. of Plant Pathology and the Genome Center; Inner Mongolia Agricultural Univ., Huhhot (China). College of Life Sciences
  5. Univ. of California, Davis, CA (United States). Dept. of Plant Pathology and the Genome Center; Joint Bioenergy Inst., Emeryville, CA (United States).
  6. Univ. of California, Davis, CA (United States). Dept. of Plant Pathology and the Genome Center; Joint Bioenergy Inst., Emeryville, CA (United States); Sichuan Agricultural Univ. at Chengdu, Wenjiang, Chengdu, Sichuan (China). Rice Research Inst.
  7. The Ohio State Univ., Columbus, OH (United States)
Publication Date:
OSTI Identifier:
1288267
Grant/Contract Number:
AC02-05CH11231
Type:
Accepted Manuscript
Journal Name:
PLoS Genetics
Additional Journal Information:
Journal Volume: 12; Journal Issue: 5; Journal ID: ISSN 1553-7404
Publisher:
Public Library of Science
Research Org:
Univ. of California, Davis, CA (United States)
Sponsoring Org:
USDOE Office of Science (SC)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES systemic acquired-resistance; enhanced disease resistance; lesion mimic mutants; salicylic-acid; transcription factors; arabidopsis npr1; pseudomonas-syringae; negative regulator; xanthomonas-oryzae; btb/poz domain