Identification and characterization of a cis-regulatory element for zygotic gene expression in Chlamydomonas reinhardtii
Abstract
Upon fertilization Chlamydomonas reinhardtii zygotes undergo a program of differentiation into a diploid zygospore that is accompanied by transcription of hundreds of zygote-specific genes. We identified a distinct sequence motif we term a zygotic response element (ZYRE) that is highly enriched in promoter regions of C. reinhardtii early zygotic genes. A luciferase reporter assay was used to show that native ZYRE motifs within the promoter of zygotic gene ZYS3 or intron of zygotic gene DMT4 are necessary for zygotic induction. A synthetic luciferase reporter with a minimal promoter was used to show that ZYRE motifs introduced upstream are sufficient to confer zygotic upregulation, and that ZYRE-controlled zygotic transcription is dependent on the homeodomain transcription factor GSP1. Furthermore, we predict that ZYRE motifs will correspond to binding sites for the homeodomain proteins GSP1-GSM1 that heterodimerize and activate zygotic gene expression in early zygotes.
- Authors:
-
- Donald Danforth Plant Science Center, St Louis, MO (United States)
- Univ. of California, Los Angeles, Los Angeles, CA (United States). Dept. of Molecular, Cell, and Developmental Biology
- Publication Date:
- Research Org.:
- Donald Danforth Plant Science Center, St. Louis, MO (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1287328
- Grant/Contract Number:
- FC02-02ER63421
- Resource Type:
- Accepted Manuscript
- Journal Name:
- G3
- Additional Journal Information:
- Journal Volume: 6; Journal Issue: 6; Journal ID: ISSN 2160-1836
- Publisher:
- Genetics Society of America
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Chlamydomonas; cis-regulatory element; fertilization; homeodomain protein; zygote; mating-type locus; chloroplast dna; gamete fusion; genome; transcription; inheritance; evolution; promoter; reporter
Citation Formats
Hamaji, Takashi, Lopez, David, Pellegrini, Matteo, and Umen, James. Identification and characterization of a cis-regulatory element for zygotic gene expression in Chlamydomonas reinhardtii. United States: N. p., 2016.
Web. doi:10.1534/g3.116.029181.
Hamaji, Takashi, Lopez, David, Pellegrini, Matteo, & Umen, James. Identification and characterization of a cis-regulatory element for zygotic gene expression in Chlamydomonas reinhardtii. United States. https://doi.org/10.1534/g3.116.029181
Hamaji, Takashi, Lopez, David, Pellegrini, Matteo, and Umen, James. Sat .
"Identification and characterization of a cis-regulatory element for zygotic gene expression in Chlamydomonas reinhardtii". United States. https://doi.org/10.1534/g3.116.029181. https://www.osti.gov/servlets/purl/1287328.
@article{osti_1287328,
title = {Identification and characterization of a cis-regulatory element for zygotic gene expression in Chlamydomonas reinhardtii},
author = {Hamaji, Takashi and Lopez, David and Pellegrini, Matteo and Umen, James},
abstractNote = {Upon fertilization Chlamydomonas reinhardtii zygotes undergo a program of differentiation into a diploid zygospore that is accompanied by transcription of hundreds of zygote-specific genes. We identified a distinct sequence motif we term a zygotic response element (ZYRE) that is highly enriched in promoter regions of C. reinhardtii early zygotic genes. A luciferase reporter assay was used to show that native ZYRE motifs within the promoter of zygotic gene ZYS3 or intron of zygotic gene DMT4 are necessary for zygotic induction. A synthetic luciferase reporter with a minimal promoter was used to show that ZYRE motifs introduced upstream are sufficient to confer zygotic upregulation, and that ZYRE-controlled zygotic transcription is dependent on the homeodomain transcription factor GSP1. Furthermore, we predict that ZYRE motifs will correspond to binding sites for the homeodomain proteins GSP1-GSM1 that heterodimerize and activate zygotic gene expression in early zygotes.},
doi = {10.1534/g3.116.029181},
journal = {G3},
number = 6,
volume = 6,
place = {United States},
year = {Sat Mar 26 00:00:00 EDT 2016},
month = {Sat Mar 26 00:00:00 EDT 2016}
}
Web of Science
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