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This content will become publicly available on January 21, 2017

Title: Enhancing metaproteomics-The value of models and defined environmental microbial systems

Metaproteomicsthe large-scale characterization of the entire protein complement of environmental microbiota at a given point in timehas provided new features to study complex microbial communities in order to unravel these black boxes. Some new technical challenges arose that were not an issue for classical proteome analytics before that could be tackled by the application of different model systems. Here, we review different current and future model systems for metaproteome analysis. We introduce model systems for clinical and biotechnological research questions including acid mine drainage, anaerobic digesters, and activated sludge, following a short introduction to microbial communities and metaproteomics. Model systems are useful to evaluate the challenges encountered within (but not limited to) metaproteomics, including species complexity and coverage, biomass availability, or reliable protein extraction. Moreover, the implementation of model systems can be considered as a step forward to better understand microbial community responses and ecological functions of single member organisms. In the future, improvements are necessary to fully explore complex environmental systems by metaproteomics.
Authors:
 [1] ;  [2] ;  [1] ;  [2] ;  [3] ;  [4] ;  [1] ;  [5] ;  [6] ;  [1]
  1. Aalborg Univ. (Denmark)
  2. Helmholtz Centre for Environmental Research, Leipzig (Germany)
  3. Univ. of Kiel (Germany)
  4. Aalborg Univ. (Denmark); Helmholtz Centre for Environmental Research, Leipzig (Germany)
  5. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  6. Univ. of Hohenheim, Stuttgart (Germany)
Publication Date:
OSTI Identifier:
1286990
Grant/Contract Number:
AC05-00OR22725
Type:
Accepted Manuscript
Journal Name:
Proteomics
Additional Journal Information:
Journal Volume: 16; Journal Issue: 5; Journal ID: ISSN 1615-9853
Publisher:
Wiley
Research Org:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES Community proteomics; Metaproteomics; Microbiology; Microbiota; Model systems; BIOLOGICAL PHOSPHORUS REMOVAL; WASTE-WATER TREATMENT; IN-VITRO MODEL; EXTRACELLULAR POLYMERIC SUBSTANCES; SCALE CONSTRUCTED WETLAND; INNATE IMMUNE-SYSTEM; HUMAN GUT MICROBIOTA