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Title: In Situ Infrared Ellipsometry for Protein Adsorption Studies on Ultrathin Smart Polymer Brushes in Aqueous Environment

The protein-adsorbing and -repelling properties of various smart nanometer-thin polymer brushes with high potential for biosensing and biomedical applications are studied by in-situ infrared-spectroscopic ellipsometry (IRSE). IRSE as a highly sensitive non-destructive technique allows us to investigate protein adsorption on polymer brushes in aqueous environment in dependence of external stimuli like temperature and pH. These stimuli are, for instance, relevant in switchable mixed brushes containing poly(N-isopropyl acrylamide) and poly(acrylic acid), respectively. We use such brushes as model surfaces for controlling protein adsorption of human serum albumin and human fibrinogen. IRSE can distinguish between polymer-specific vibrational bands, which yield insights into the hydration state of the brushes, and changes in the protein-specific amide bands, which are related to changes of the protein secondary structure.
Authors:
 [1] ;  [1] ;  [1] ;  [2] ;  [2] ;  [2] ;  [2] ;  [3] ;  [3] ;  [4] ;  [5] ;  [6] ;  [1]
  1. Leibniz Inst. for Analytical sciences (ISAS), Berlin (Germany)
  2. Leibniz Inst. of Polymer Research (IPF), Dresden (Germany)
  3. Clemson Univ., SC (United States). School of Materials Science and Engineering
  4. Univ. of Tennessee, Knoxville, TN (United States). Dept. of Chemistry and of Chemical Engineering
  5. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Center for Nanophase Materials Science (CNMS)
  6. Univ. of Georgia, Athens, GA (United States). Dept. of Chemistry
Publication Date:
OSTI Identifier:
1286795
Grant/Contract Number:
AC05-00OR22725; DMR-1107786; DFG HI 793/4-1
Type:
Accepted Manuscript
Journal Name:
ACS Applied Materials and Interfaces
Additional Journal Information:
Journal Volume: 7; Journal Issue: 23; Journal ID: ISSN 1944-8244
Publisher:
American Chemical Society
Research Org:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). Center for Nanophase Materials Sciences (CNMS)
Sponsoring Org:
USDOE Office of Science (SC); German Research Foundation; National Science Foundation (NSF)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 36 MATERIALS SCIENCE in situ infrared ellipsometry; infrared spectroscopy; polymer brushes; protein adsorption; solid−liquid interface; stimuli-responsive