Modular microfluidics for point-of-care protein purifications
Abstract
Biochemical separations are the heart of diagnostic assays and purification methods for biologics. On-chip miniaturization and modularization of separation procedures will enable the development of customized, portable devices for personalized health-care diagnostics and point-of-use production of treatments. In this report, we describe the design and fabrication of miniature ion exchange, size exclusion and affinity chromatography modules for on-chip clean-up of recombinantly-produced proteins. Our results demonstrate that these common separations techniques can be implemented in microfluidic modules with performance comparable to conventional approaches. We introduce embedded 3-D microfluidic interconnects for integrating micro-scale separation modules that can be arranged and reconfigured to suit a variety of fluidic operations or biochemical processes. In conclusion, we demonstrate the utility of the modular approach with a platform for the enrichment of enhanced green fluorescent protein (eGFP) from Escherichia coli lysate through integrated affinity and size-exclusion chromatography modules.
- Authors:
-
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Center for Nanophase Materials Science (CNMS)
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). Center for Nanophase Materials Sciences (CNMS)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES); Work for Others (WFO); Defense Advanced Research Projects Agency (DARPA)
- OSTI Identifier:
- 1286752
- Grant/Contract Number:
- AC05-00OR22725; HR001134005
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Lab on a Chip
- Additional Journal Information:
- Journal Volume: 15; Journal Issue: 8; Journal ID: ISSN 1473-0197
- Publisher:
- Royal Society of Chemistry
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Citation Formats
Millet, L. J., Lucheon, J. D., Standaert, R. F., Retterer, S. T., and Doktycz, M. J. Modular microfluidics for point-of-care protein purifications. United States: N. p., 2015.
Web. doi:10.1039/C5LC00094G.
Millet, L. J., Lucheon, J. D., Standaert, R. F., Retterer, S. T., & Doktycz, M. J. Modular microfluidics for point-of-care protein purifications. United States. https://doi.org/10.1039/C5LC00094G
Millet, L. J., Lucheon, J. D., Standaert, R. F., Retterer, S. T., and Doktycz, M. J. Thu .
"Modular microfluidics for point-of-care protein purifications". United States. https://doi.org/10.1039/C5LC00094G. https://www.osti.gov/servlets/purl/1286752.
@article{osti_1286752,
title = {Modular microfluidics for point-of-care protein purifications},
author = {Millet, L. J. and Lucheon, J. D. and Standaert, R. F. and Retterer, S. T. and Doktycz, M. J.},
abstractNote = {Biochemical separations are the heart of diagnostic assays and purification methods for biologics. On-chip miniaturization and modularization of separation procedures will enable the development of customized, portable devices for personalized health-care diagnostics and point-of-use production of treatments. In this report, we describe the design and fabrication of miniature ion exchange, size exclusion and affinity chromatography modules for on-chip clean-up of recombinantly-produced proteins. Our results demonstrate that these common separations techniques can be implemented in microfluidic modules with performance comparable to conventional approaches. We introduce embedded 3-D microfluidic interconnects for integrating micro-scale separation modules that can be arranged and reconfigured to suit a variety of fluidic operations or biochemical processes. In conclusion, we demonstrate the utility of the modular approach with a platform for the enrichment of enhanced green fluorescent protein (eGFP) from Escherichia coli lysate through integrated affinity and size-exclusion chromatography modules.},
doi = {10.1039/C5LC00094G},
journal = {Lab on a Chip},
number = 8,
volume = 15,
place = {United States},
year = {Thu Jan 01 00:00:00 EST 2015},
month = {Thu Jan 01 00:00:00 EST 2015}
}
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