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Title: Steering microtubule shuttle transport with dynamically controlled magnetic fields

Abstract

Nanoscale control of matter is critical to the design of integrated nanosystems. Here, we describe a method to dynamically control directionality of microtubule (MT) motion using programmable magnetic fields. MTs are combined with magnetic quantum dots (i.e., MagDots) that are manipulated by external magnetic fields provided by magnetic nanowires. MT shuttles thus undergo both ATP-driven and externally-directed motion with a fluorescence component that permits simultaneous visualization of shuttle motion. This technology is used to alter the trajectory of MTs in motion and to pin MT motion. Ultimately, such an approach could be used to evaluate the MT-kinesin transport system and could serve as the basis for improved lab-on-a-chip technologies based on MT transport.

Authors:
 [1];  [2];  [1];  [3];  [1];  [4];  [1];  [4];  [3];  [5]
+ Show Author Affiliations
  1. The Ohio State Univ., Columbus, OH (United States). William G. Lowrie Dept. of Chemical and Biomolecular Engineering
  2. The Ohio State Univ., Columbus, OH (United States). William G. Lowrie Dept. of Chemical and Biomolecular Engineering; Nanjing Univ. (China). College of Engineering and Applied Sciences, Dept. of Biomedical Engineering
  3. The Ohio State Univ., Columbus, OH (United States). Dept of Physics
  4. Sandia National Lab. (SNL-NM), Albuquerque, NM (United States). Center for Integrated Nanotechnologies
  5. The Ohio State Univ., Columbus, OH (United States). William G. Lowrie Dept. of Chemical and Biomolecular Engineering; The Ohio State Univ., Columbus, OH (United States). Dept of Biomedical Engineering
Publication Date:
Research Org.:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES); USDOE National Nuclear Security Administration (NNSA)
OSTI Identifier:
1285955
Report Number(s):
SAND2016-7202J
Journal ID: ISSN 2040-3364; NANOHL; 646152
Grant/Contract Number:  
AC04-94AL85000; CMMI-0900377; DMR-1206745; EEC-0914790; DMR-0820414; W911NF-14-1-0289
Resource Type:
Accepted Manuscript
Journal Name:
Nanoscale
Additional Journal Information:
Journal Volume: 8; Journal Issue: 16; Journal ID: ISSN 2040-3364
Publisher:
Royal Society of Chemistry
Country of Publication:
United States
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; 77 NANOSCIENCE AND NANOTECHNOLOGY

Citation Formats

Mahajan, K. D., Ruan, G., Dorcéna, C. J., Vieira, G., Nabar, G., Bouxsein, N. F., Chalmers, J. J., Bachand, G. D., Sooryakumar, R., and Winter, J. O. Steering microtubule shuttle transport with dynamically controlled magnetic fields. United States: N. p., 2016. Web. doi:10.1039/c5nr08529b.
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Mahajan, K. D., Ruan, G., Dorcéna, C. J., Vieira, G., Nabar, G., Bouxsein, N. F., Chalmers, J. J., Bachand, G. D., Sooryakumar, R., & Winter, J. O. Steering microtubule shuttle transport with dynamically controlled magnetic fields. United States. https://doi.org/10.1039/c5nr08529b
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Mahajan, K. D., Ruan, G., Dorcéna, C. J., Vieira, G., Nabar, G., Bouxsein, N. F., Chalmers, J. J., Bachand, G. D., Sooryakumar, R., and Winter, J. O. Wed . "Steering microtubule shuttle transport with dynamically controlled magnetic fields". United States. https://doi.org/10.1039/c5nr08529b. https://www.osti.gov/servlets/purl/1285955.
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@article{osti_1285955,
title = {Steering microtubule shuttle transport with dynamically controlled magnetic fields},
author = {Mahajan, K. D. and Ruan, G. and Dorcéna, C. J. and Vieira, G. and Nabar, G. and Bouxsein, N. F. and Chalmers, J. J. and Bachand, G. D. and Sooryakumar, R. and Winter, J. O.},
abstractNote = {Nanoscale control of matter is critical to the design of integrated nanosystems. Here, we describe a method to dynamically control directionality of microtubule (MT) motion using programmable magnetic fields. MTs are combined with magnetic quantum dots (i.e., MagDots) that are manipulated by external magnetic fields provided by magnetic nanowires. MT shuttles thus undergo both ATP-driven and externally-directed motion with a fluorescence component that permits simultaneous visualization of shuttle motion. This technology is used to alter the trajectory of MTs in motion and to pin MT motion. Ultimately, such an approach could be used to evaluate the MT-kinesin transport system and could serve as the basis for improved lab-on-a-chip technologies based on MT transport.},
doi = {10.1039/c5nr08529b},
journal = {Nanoscale},
number = 16,
volume = 8,
place = {United States},
year = {Wed Mar 23 00:00:00 EDT 2016},
month = {Wed Mar 23 00:00:00 EDT 2016}
}
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https://doi.org/10.1039/c5nr08529b
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    Works referencing / citing this record:

    Azobenzene‐Based Photoswitches Facilitating Reversible Regulation of Kinesin and Myosin Motor Systems for Nanotechnological Applications
    journal, April 2019

    • Amrutha, Ammathnadu S.; Sunil Kumar, K. R.; Tamaoki, Nobuyuki
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