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Title: Continuity of monolayer-bilayer junctions for localization of lipid raft microdomains in model membranes

We show that the selective localization of cholesterol-rich domains and associated ganglioside receptors prefer to occur in the monolayer across continuous monolayer-bilayer junctions (MBJs) in supported lipid membranes. For the MBJs, glass substrates were patterned with poly(dimethylsiloxane) (PDMS) oligomers by thermally-assisted contact printing, leaving behind 3 nm-thick PDMS patterns. The hydrophobicity of the transferred PDMS patterns was precisely tuned by the stamping temperature. Lipid monolayers were formed on the PDMS patterned surface while lipid bilayers were on the bare glass surface. Due to the continuity of the lipid membranes over the MBJs, essentially free diffusion of lipids was allowed between the monolayer on the PDMS surface and the upper leaflet of the bilayer on the glass substrate. The preferential localization of sphingomyelin, ganglioside GM1 and cholesterol in the monolayer region enabled to develop raft microdomains through coarsening of nanorafts. Furthermore, our methodology provides a simple and effective scheme of non-disruptive manipulation of the chemical landscape associated with lipid phase separations, which leads to more sophisticated applications in biosensors and as cell culture substrates.
Authors:
 [1] ;  [2] ;  [3] ;  [4] ;  [5] ;  [6] ;  [7] ;  [3]
  1. Seoul National Univ., Seoul (Korea). School of Electrical Engineering #032; Univ. of Minnesota, Minneapolis, MN (United States). Dept. of Electrical and Computer Engineering
  2. Univ. of Minnesota, Minneapolis, MN (United States). Dept. of Electrical and Computer Engineering
  3. Seoul National Univ., Seoul (Korea). School of Electrical Engineering #032
  4. Seoul National Univ., Seoul (Korea). School of Electrical Engineering #032; Korea Research Institute of Standards and Science, Daejeon (Republic of Korea). Division of Physical Metrology
  5. Kyung Hee Univ., Seoul (Korea). Inst. of Oriental Medicine, Dept. of Anatomy, College of Korean Medicine
  6. Univ. of Minnesota, Minneapolis, MN (United States). Dept. of Electrical and Computer Engineering, Dept. of Biomedical Engineering
  7. Univ. of California, Davis, CA (United States). Dept. of Biomedical Engineering and Chemical Engineering & Materials Science
Publication Date:
OSTI Identifier:
1285912
Grant/Contract Number:
FG02-04ER46173
Type:
Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 6; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Research Org:
University of California, Davis
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES surface; proteins; cholesterol; domains; poly(dimethylsiloxane); polydimethylsiloxane; reconstitution; manipulation; diffusion; oligomers