Immobilization of cellulase on a silica gel substrate modified using a 3-APTES self-assembled monolayer

Zhang, Dezhi; Hegab, Hisham E.; Lvov, Yuri; Dale Snow, L.; Palmer, James

doi:10.1186/s40064-016-1682-y

Title: Immobilization of cellulase on a silica gel substrate modified using a 3-APTES self-assembled monolayer

Abstract

Cellulase was immobilized onto silica gel surfaces pretreated with (3-aminopropyl) triethoxy-silane (3-APTES), and glutaraldehyde (GA) was used as a cross-linker. A carboxymethyl cellulose sodium salt (CMC) solution was used for activity experiments. Protein assay was performed to determine the mass immobilized and compare with free enzyme. Cellulase was successfully demonstrated to be immobilized on the modified silica gel surface, and no detectable amount of enzyme was stripped off during the hydrolysis of the CMC solution. The specific activity of the immobilized cellulase is 7 ± 2 % compared to the similar amount of free cellulase. Significant activity over multiple reuses was observed. The seventh batch achieved 82 % activity of the initial batch, and the fifteenth batch retained 31 %. Lastly, it was observed that the immobilized cellulase retained 48 % of its initial activity after 4 days, and 22 % even after 14 days.

Authors:: Zhang, Dezhi; Hegab, Hisham E.; Lvov, Yuri; Dale Snow, L.; Palmer, James

Publication Date:: Wed Jan 20 00:00:00 EST 2016

Research Org.:: Louisiana Tech Univ., Ruston, LA (United States)

Sponsoring Org.:: USDOE Office of Energy Efficiency and Renewable Energy (EERE)

OSTI Identifier:: 1272638

Alternate Identifier(s):: OSTI ID: 1242890

Grant/Contract Number:: EE0003120

Resource Type:: Published Article

Journal Name:: SpringerPlus

Additional Journal Information:: Journal Name: SpringerPlus Journal Volume: 5 Journal Issue: 1; Journal ID: ISSN 2193-1801

Publisher:: Springer Science + Business Media

Country of Publication:: Switzerland

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; cellulase; (3-Aminopropyl) triethoxy-silane; glutaraldehyde; glucose; silica gel

Citation Formats


                    Zhang, Dezhi, Hegab, Hisham E., Lvov, Yuri, Dale Snow, L., and Palmer, James. Immobilization of cellulase on a silica gel substrate modified using a 3-APTES self-assembled monolayer.  Switzerland: N. p., 2016. 
Web.  doi:10.1186/s40064-016-1682-y.

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                    Zhang, Dezhi, Hegab, Hisham E., Lvov, Yuri, Dale Snow, L., & Palmer, James. Immobilization of cellulase on a silica gel substrate modified using a 3-APTES self-assembled monolayer.  Switzerland.  https://doi.org/10.1186/s40064-016-1682-y

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                    Zhang, Dezhi, Hegab, Hisham E., Lvov, Yuri, Dale Snow, L., and Palmer, James. Wed .  
"Immobilization of cellulase on a silica gel substrate modified using a 3-APTES self-assembled monolayer".  Switzerland.  https://doi.org/10.1186/s40064-016-1682-y.

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@article{osti_1272638,

  title        = {Immobilization of cellulase on a silica gel substrate modified using a 3-APTES self-assembled monolayer},

  author       = {Zhang, Dezhi and Hegab, Hisham E. and Lvov, Yuri and Dale Snow, L. and Palmer, James},

  abstractNote = {Cellulase was immobilized onto silica gel surfaces pretreated with (3-aminopropyl) triethoxy-silane (3-APTES), and glutaraldehyde (GA) was used as a cross-linker. A carboxymethyl cellulose sodium salt (CMC) solution was used for activity experiments. Protein assay was performed to determine the mass immobilized and compare with free enzyme. Cellulase was successfully demonstrated to be immobilized on the modified silica gel surface, and no detectable amount of enzyme was stripped off during the hydrolysis of the CMC solution. The specific activity of the immobilized cellulase is 7 ± 2 % compared to the similar amount of free cellulase. Significant activity over multiple reuses was observed. The seventh batch achieved 82 % activity of the initial batch, and the fifteenth batch retained 31 %. Lastly, it was observed that the immobilized cellulase retained 48 % of its initial activity after 4 days, and 22 % even after 14 days.},

  doi          = {10.1186/s40064-016-1682-y},

  journal      = {SpringerPlus},

  number       = 1,

  volume       = 5,

  place        = {Switzerland},

  year         = {Wed Jan 20 00:00:00 EST 2016},

  month        = {Wed Jan 20 00:00:00 EST 2016}

}

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Works referenced in this record:

Enzymatic hydrolysis and fermentation of palm kernel press cake for production of bioethanol
journal, March 2010

Cerveró, José María; Skovgaard, Pernille Anastasia; Felby, Claus
Enzyme and Microbial Technology, Vol. 46, Issue 3-4
DOI: 10.1016/j.enzmictec.2009.10.012

Simple and efficient synthesis of copper(II)-modified uniform magnetic Fe3O4@SiO2 core/shell microspheres for immobilization of cellulase
journal, October 2013

Li, Shi-Kuo; Hou, Xiao-Cheng; Huang, Fang-Zhi
Journal of Nanoparticle Research, Vol. 15, Issue 11
DOI: 10.1007/s11051-013-2013-7

Hydrolytic Activity of Free and Immobilized Cellulase
journal, February 2009

Tébéka, Iris R. M.; Silva, Artur G. L.; Petri, Denise F. S.
Langmuir, Vol. 25, Issue 3
DOI: 10.1021/la802882s

Synthesis and lysozyme adsorption of rod-like large-pore periodic mesoporous organosilica
journal, July 2006

Qiao, S. Z.; Djojoputro, Hiannie; Hu, Qiuhong
Progress in Solid State Chemistry, Vol. 34, Issue 2-4
DOI: 10.1016/j.progsolidstchem.2005.11.023

Kinetics of cellulose saccharification by Trichoderma reesei cellulases
journal, January 2001

Ortega, N.
International Biodeterioration & Biodegradation, Vol. 47, Issue 1, p. 7-14
DOI: 10.1016/S0964-8305(00)00101-3

Self-organized proton conductive layers in hybrid proton exchange membranes, exhibiting high ionic conductivity
journal, January 2009

Michau, Mathieu; Barboiu, Mihail
Journal of Materials Chemistry, Vol. 19, Issue 34
DOI: 10.1039/b908680c

Confirmation of the presence of imine bonds in thermally cured polyimides
journal, October 1993

Saini, Ajay K.; Carlin, Clifford M.; Patterson, Howard H.
Journal of Polymer Science Part A: Polymer Chemistry, Vol. 31, Issue 11
DOI: 10.1002/pola.1993.080311111

Enzyme immobilization in a biomimetic silica support
journal, January 2004

Luckarift, Heather R.; Spain, Jim C.; Naik, Rajesh R.
Nature Biotechnology, Vol. 22, Issue 2
DOI: 10.1038/nbt931

Characterization of direct cellulase immobilization with superparamagnetic nanoparticles
journal, January 2011

Xu, Jingliang; Huo, Shuhao; Yuan, Zhenhong
Biocatalysis and Biotransformation, Vol. 29, Issue 2-3
DOI: 10.3109/10242422.2011.566326

Imino-chitosan biodynamers
journal, January 2012

Marin, Luminita; Simionescu, Bogdan; Barboiu, Mihail
Chemical Communications, Vol. 48, Issue 70
DOI: 10.1039/c2cc34337a

Production of liquid biofuels from renewable resources
journal, February 2011

Nigam, Poonam Singh; Singh, Anoop
Progress in Energy and Combustion Science, Vol. 37, Issue 1
DOI: 10.1016/j.pecs.2010.01.003

Preparation of Magnetic Chitosan Nanoparticles As Support for Cellulase Immobilization
journal, February 2014

Zang, Limin; Qiu, Jianhui; Wu, Xueli
Industrial & Engineering Chemistry Research, Vol. 53, Issue 9
DOI: 10.1021/ie404072s

Preparation of magnetic porous terpolymer and its application in cellulase immobilization
journal, June 2014

Qi, Huimin; Duan, Hongdong; Wang, Xingjian
Polymer Engineering & Science, Vol. 55, Issue 5
DOI: 10.1002/pen.23973

IMMOBILIZATION OF CELLULASES ON AMINE AND ALDEHYDE FUNCTIONALIZED Fe ₂ O ₃ MAGNETIC NANOPARTICLES
journal, May 2012

Alahakoon, Thilini; Koh, Jun Wei; Chong, Xiang Wen Cindy
Preparative Biochemistry and Biotechnology, Vol. 42, Issue 3
DOI: 10.1080/10826068.2011.602800

Study of cellulolytic enzyme immobilization on copolymers of N-vinylformamide
journal, October 2015

Tąta, Agnieszka; Sokołowska, Katarzyna; Świder, Joanna
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, Vol. 149
DOI: 10.1016/j.saa.2015.04.112

Enzyme Immobilization: The Quest for Optimum Performance
journal, June 2007

Sheldon, Roger A.
Advanced Synthesis & Catalysis, Vol. 349, Issue 8-9
DOI: 10.1002/adsc.200700082

Encapsulation of cellulase with mesoporous silica (SBA-15)
journal, December 2008

Takimoto, Anri; Shiomi, Toru; Ino, Keita
Microporous and Mesoporous Materials, Vol. 116, Issue 1-3
DOI: 10.1016/j.micromeso.2008.05.046

Fuel Ethanol from Cellulosic Biomass
journal, March 1991

Lynd, L. R.; Cushman, J. H.; Nichols, R. J.
Science, Vol. 251, Issue 4999
DOI: 10.1126/science.251.4999.1318

Improving the stability of cellulase by immobilization on modified polyvinyl alcohol coated chitosan beads
journal, April 2007

Dinçer, Ayşe; Telefoncu, Azmi
Journal of Molecular Catalysis B: Enzymatic, Vol. 45, Issue 1-2
DOI: 10.1016/j.molcatb.2006.10.005

Enzyme Immobilization in Biotechnology
journal, November 2008

Spahn, Cynthia; Minteer, Shelley
Recent Patents on Engineering, Vol. 2, Issue 3
DOI: 10.2174/187221208786306333

Structure of 3-aminopropyl triethoxy silane on silicon oxide
journal, November 1991

Vandenberg, Elaine T.; Bertilsson, Lars; Liedberg, Bo
Journal of Colloid and Interface Science, Vol. 147, Issue 1
DOI: 10.1016/0021-9797(91)90139-Y

Immobilization of cellulase on magnetoresponsive graphene nano-supports
journal, June 2013

Gokhale, Ankush A.; Lu, Jue; Lee, Ilsoon
Journal of Molecular Catalysis B: Enzymatic, Vol. 90
DOI: 10.1016/j.molcatb.2013.01.025

Bioethanol from lignocellulosics: Status and perspectives in Canada
journal, July 2010

Mabee, W. E.; Saddler, J. N.
Bioresource Technology, Vol. 101, Issue 13
DOI: 10.1016/j.biortech.2009.10.098

Cellulase immobilized by sol–gel entrapment for efficient hydrolysis of cellulose
journal, October 2012

Ungurean, Mihaela; Paul, Cristina; Peter, Francisc
Bioprocess and Biosystems Engineering, Vol. 36, Issue 10
DOI: 10.1007/s00449-012-0835-9

A novel method to prepare chitosan powder and its application in cellulase immobilization
journal, January 2006

Mao, Xuepu; Guo, Gangjun; Huang, Jinfeng
Journal of Chemical Technology & Biotechnology, Vol. 81, Issue 2
DOI: 10.1002/jctb.1378

Size selective protein adsorption on thiol-functionalised SBA-15 mesoporous molecular sieve
journal, January 2001

Yiu, Humphrey H. P.; Botting, Catherine H.; Botting, Nigel P.
Physical Chemistry Chemical Physics, Vol. 3, Issue 15
DOI: 10.1039/b104729a

Immobilization of Aspergillus nidulans SU04 cellulase on modified activated carbon: Sorption and kinetic studies
journal, July 2011

Anuradha Jabasingh, S.; Valli Nachiyar, C.
Journal of Thermal Analysis and Calorimetry, Vol. 109, Issue 1
DOI: 10.1007/s10973-011-1758-4

Enzyme immobilization in MCM-41 molecular sieve
journal, December 1996

Díaz, J. Felipe; Balkus, Kenneth J.
Journal of Molecular Catalysis B: Enzymatic, Vol. 2, Issue 2-3
DOI: 10.1016/S1381-1177(96)00017-3

Sorption and immobilization of cellulase on silicate clay minerals
journal, October 2005

Safari Sinegani, Ali Akbar; Emtiazi, Giti; Shariatmadari, Hossain
Journal of Colloid and Interface Science, Vol. 290, Issue 1
DOI: 10.1016/j.jcis.2005.04.030

Active biocatalysts based on Candida rugosa lipase immobilized in vesicular silica
journal, June 2012

Wu, Cuicui; Zhou, Guowei; Jiang, Xiaojie
Process Biochemistry, Vol. 47, Issue 6
DOI: 10.1016/j.procbio.2012.03.004

Functionalized Mesoporous Silica with Very Large Pores for Cellulase Immobilization
journal, April 2010

Hartono, Sandy Budi; Qiao, Shi Zhang; Liu, Jian
The Journal of Physical Chemistry C, Vol. 114, Issue 18
DOI: 10.1021/jp102368s

Cross-Linked Enzyme Aggregates as Industrial Biocatalysts
journal, January 2011

Sheldon, Roger A.
Organic Process Research & Development, Vol. 15, Issue 1
DOI: 10.1021/op100289f

Exploring novel ultrafine Eri silk bioscaffold for enzyme stabilisation in cellobiose hydrolysis
journal, October 2013

Verma, Madan L.; Rajkhowa, Rangam; Wang, Xungai
Bioresource Technology, Vol. 145
DOI: 10.1016/j.biortech.2013.01.065

Increase in stability of cellulase immobilized on functionalized magnetic nanospheres
journal, February 2015

Zhang, Wenjuan; Qiu, Jianhui; Feng, Huixia
Journal of Magnetism and Magnetic Materials, Vol. 375
DOI: 10.1016/j.jmmm.2014.09.067

Enzymes supported on ordered mesoporous solids: a special case of an inorganic–organic hybrid
journal, January 2005

Yiu, Humphrey H. P.; Wright, Paul A.
Journal of Materials Chemistry, Vol. 15, Issue 35-36
DOI: 10.1039/b506090g

Ordered Mesoporous Silica with Large Cage-Like Pores: Structural Identification and Pore Connectivity Design by Controlling the Synthesis Temperature and Time
journal, January 2003

Matos, Jivaldo R.; Kruk, Michal; Mercuri, Lucildes P.
Journal of the American Chemical Society, Vol. 125, Issue 3
DOI: 10.1021/ja0283347

Characterization and immobilization of liposome-bound cellulase for hydrolysis of insoluble cellulose
journal, May 2007

Li, Chengzhou; Yoshimoto, Makoto; Fukunaga, Kimitoshi
Bioresource Technology, Vol. 98, Issue 7
DOI: 10.1016/j.biortech.2006.05.028

Cellulase immobilized mesoporous silica nanocatalysts for efficient cellulose-to-glucose conversion
journal, January 2011

Chang, Renee Han-Yi; Jang, Jen; Wu, Kevin C. -W.
Green Chemistry, Vol. 13, Issue 10
DOI: 10.1039/c1gc15563f

Covalent Immobilization of Cellulases onto a Water-Soluble–Insoluble Reversible Polymer
journal, January 2012

Yu, Yuanyuan; Yuan, Jiugang; Wang, Qiang
Applied Biochemistry and Biotechnology, Vol. 166, Issue 6
DOI: 10.1007/s12010-011-9536-0

Activity of glucose oxidase entrapped in mesoporous gels
journal, January 2005

Han, Kicheol; Wu, Zhijian; Lee, Joun
Biochemical Engineering Journal, Vol. 22, Issue 2
DOI: 10.1016/j.bej.2004.09.011

Comparative Life-Cycle Assessments for Biomass-to-Ethanol Production from Different Regional Feedstocks
journal, January 2005

Kemppainen, Amber J.; Shonnard, David R.
Biotechnology Progress, Vol. 21, Issue 4
DOI: 10.1021/bp049548q

Improving Adsorbent Properties of Cage-like Ordered Amine Functionalized Mesoporous Silica with Very Large Pores for Bioadsorption
journal, June 2009

Budi Hartono, Sandy; Qiao, ShiZhang; Jack, Kevin
Langmuir, Vol. 25, Issue 11
DOI: 10.1021/la900023p

Immobilization of cellulase enzyme on functionalized multiwall carbon nanotubes
journal, September 2014

Mubarak, N. M.; Wong, J. R.; Tan, K. W.
Journal of Molecular Catalysis B: Enzymatic, Vol. 107
DOI: 10.1016/j.molcatb.2014.06.002

Outlook for cellulase improvement: Screening and selection strategies
journal, September 2006

Percival Zhang, Y.-H.; Himmel, Michael E.; Mielenz, Jonathan R.
Biotechnology Advances, Vol. 24, Issue 5, p. 452-481
DOI: 10.1016/j.biotechadv.2006.03.003

Retention of Enzymatic Activity of α-Amylase in the Reductive Synthesis of Gold Nanoparticles
journal, May 2007

Rangnekar, Abhijit; Sarma, Tridib Kumar; Singh, Atul Kumar
Langmuir, Vol. 23, Issue 10
DOI: 10.1021/la062749e

Suitability of magnetic nanoparticle immobilised cellulases in enhancing enzymatic saccharification of pretreated hemp biomass
journal, January 2014

Abraham, Reinu E.; Verma, Madan L.; Barrow, Colin J.
Biotechnology for Biofuels, Vol. 7, Issue 1
DOI: 10.1186/1754-6834-7-90

Cellulase assisted synthesis of nano-silver and gold: Application as immobilization matrix for biocatalysis
journal, June 2015

Mishra, Abhijeet; Sardar, Meryam
International Journal of Biological Macromolecules, Vol. 77
DOI: 10.1016/j.ijbiomac.2015.03.014

Plant genetic engineering for biofuel production: towards affordable cellulosic ethanol
journal, June 2008

Sticklen, Mariam B.
Nature Reviews Genetics, Vol. 9, Issue 6
DOI: 10.1038/nrg2336

Fabrication of graphene oxide decorated with Fe3O4@SiO2 for immobilization of cellulase
journal, January 2015

Li, Yue; Wang, Xiang-Yu; Jiang, Xiao-Ping
Journal of Nanoparticle Research, Vol. 17, Issue 1
DOI: 10.1007/s11051-014-2826-z

Characterization of functionalized nanoporous supports for protein confinement
journal, October 2006

Lei, Chenghong; Shin, Yongsoon; Magnuson, Jon K.
Nanotechnology, Vol. 17, Issue 22
DOI: 10.1088/0957-4484/17/22/001

Synthesis and Bio-adsorptive Properties of Large-Pore Periodic Mesoporous Organosilica Rods
journal, November 2005

Qiao, S. Z.; Yu, C. Z.; Xing, W.
Chemistry of Materials, Vol. 17, Issue 24
DOI: 10.1021/cm051735b

Works referencing / citing this record:

Physicochemical and catalytic properties of acylase I from aspergillus melleus immobilized on amino- and carbonyl-grafted stöber silica
journal, January 2018

Kołodziejczak-Radzimska, Agnieszka; Zdarta, Jakub; Jesionowski, Teofil
Biotechnology Progress, Vol. 34, Issue 3
DOI: 10.1002/btpr.2610

Magnetic Recovery of Cellulase from Cellulose Substrates with Bare Iron Oxide Nanoparticles
journal, January 2019

Schnell, Florian; Kube, Massimo; Berensmeier, Sonja
ChemNanoMat, Vol. 5, Issue 4
DOI: 10.1002/cnma.201800658

Inulinase immobilization on polyethylene glycol/polypyrrole multiwall carbon nanotubes producing a catalyst with enhanced thermal and operational stability
journal, July 2019

Temkov, Mishela; Petrovski, Aleksandar; Gjorgieva, Emilija
Engineering in Life Sciences, Vol. 19, Issue 9
DOI: 10.1002/elsc.201900021

Immobilization of alcohol dehydrogenase on ceramic silicon carbide membranes for enzymatic CH ₃ OH production: ADH immobilization on silicon carbide
journal, May 2018

Zeuner, Birgitte; Ma, Nicolaj; Berendt, Kasper
Journal of Chemical Technology & Biotechnology, Vol. 93, Issue 10
DOI: 10.1002/jctb.5653

Fabrication of iron oxide nanoparticles, and green catalytic application of an immobilized novel iron Schiff on wood cellulose
journal, December 2017

Tajik, Elham; Naeimi, Atena; Amiri, Asghar
Cellulose, Vol. 25, Issue 2
DOI: 10.1007/s10570-017-1615-0

Continuous flow biocatalysis
journal, January 2018

Britton, Joshua; Majumdar, Sudipta; Weiss, Gregory A.
Chemical Society Reviews, Vol. 47, Issue 15
DOI: 10.1039/c7cs00906b

QCM-D and NanoTweezer measurements to characterize the effect of soil cellulase on the deposition of PEG-coated TiO ₂ nanoparticles in model subsurface environments
journal, January 2018

Akanbi, M. O.; Hernandez, L. M.; Mobarok, M. H.
Environmental Science: Nano, Vol. 5, Issue 9
DOI: 10.1039/c8en00508g

Optical and structural properties of curcuminoids extracted from Curcuma longa L. for hybrid white light diode
journal, October 2018

Al Shafouri, M.; Ahmed, Naser M.; Hassan, Z.
The European Physical Journal Applied Physics, Vol. 84, Issue 1
DOI: 10.1051/epjap/2018180185

A study on the enzymatic properties and reuse of cellulase immobilized with carbon nanotubes and sodium alginate
journal, July 2019

Li, Li-Juan; Xia, Wen-Jing; Ma, Gui-Ping
AMB Express, Vol. 9, Issue 1
DOI: 10.1186/s13568-019-0835-0

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Title: Immobilization of cellulase on a silica gel substrate modified using a 3-APTES self-assembled monolayer

Abstract

Citation Formats

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Cellulase immobilized mesoporous silica nanocatalysts for efficient cellulose-to-glucose conversion journal, January 2011

Covalent Immobilization of Cellulases onto a Water-Soluble–Insoluble Reversible Polymer journal, January 2012

Activity of glucose oxidase entrapped in mesoporous gels journal, January 2005

Comparative Life-Cycle Assessments for Biomass-to-Ethanol Production from Different Regional Feedstocks journal, January 2005

Improving Adsorbent Properties of Cage-like Ordered Amine Functionalized Mesoporous Silica with Very Large Pores for Bioadsorption journal, June 2009

Immobilization of cellulase enzyme on functionalized multiwall carbon nanotubes journal, September 2014

Outlook for cellulase improvement: Screening and selection strategies journal, September 2006

Retention of Enzymatic Activity of α-Amylase in the Reductive Synthesis of Gold Nanoparticles journal, May 2007

Suitability of magnetic nanoparticle immobilised cellulases in enhancing enzymatic saccharification of pretreated hemp biomass journal, January 2014

Cellulase assisted synthesis of nano-silver and gold: Application as immobilization matrix for biocatalysis journal, June 2015

Plant genetic engineering for biofuel production: towards affordable cellulosic ethanol journal, June 2008

Fabrication of graphene oxide decorated with Fe3O4@SiO2 for immobilization of cellulase journal, January 2015

Characterization of functionalized nanoporous supports for protein confinement journal, October 2006

Synthesis and Bio-adsorptive Properties of Large-Pore Periodic Mesoporous Organosilica Rods journal, November 2005

Physicochemical and catalytic properties of acylase I from aspergillus melleus immobilized on amino- and carbonyl-grafted stöber silica journal, January 2018

Magnetic Recovery of Cellulase from Cellulose Substrates with Bare Iron Oxide Nanoparticles journal, January 2019

Inulinase immobilization on polyethylene glycol/polypyrrole multiwall carbon nanotubes producing a catalyst with enhanced thermal and operational stability journal, July 2019

Immobilization of alcohol dehydrogenase on ceramic silicon carbide membranes for enzymatic CH 3 OH production: ADH immobilization on silicon carbide journal, May 2018

Fabrication of iron oxide nanoparticles, and green catalytic application of an immobilized novel iron Schiff on wood cellulose journal, December 2017

Continuous flow biocatalysis journal, January 2018

QCM-D and NanoTweezer measurements to characterize the effect of soil cellulase on the deposition of PEG-coated TiO 2 nanoparticles in model subsurface environments journal, January 2018

Optical and structural properties of curcuminoids extracted from Curcuma longa L. for hybrid white light diode journal, October 2018

A study on the enzymatic properties and reuse of cellulase immobilized with carbon nanotubes and sodium alginate journal, July 2019

Enzymatic hydrolysis and fermentation of palm kernel press cake for production of bioethanol
journal, March 2010

Simple and efficient synthesis of copper(II)-modified uniform magnetic Fe3O4@SiO2 core/shell microspheres for immobilization of cellulase
journal, October 2013

Hydrolytic Activity of Free and Immobilized Cellulase
journal, February 2009

Synthesis and lysozyme adsorption of rod-like large-pore periodic mesoporous organosilica
journal, July 2006

Kinetics of cellulose saccharification by Trichoderma reesei cellulases
journal, January 2001

Self-organized proton conductive layers in hybrid proton exchange membranes, exhibiting high ionic conductivity
journal, January 2009

Confirmation of the presence of imine bonds in thermally cured polyimides
journal, October 1993

Enzyme immobilization in a biomimetic silica support
journal, January 2004

Characterization of direct cellulase immobilization with superparamagnetic nanoparticles
journal, January 2011

Imino-chitosan biodynamers
journal, January 2012

Production of liquid biofuels from renewable resources
journal, February 2011

Preparation of Magnetic Chitosan Nanoparticles As Support for Cellulase Immobilization
journal, February 2014

Preparation of magnetic porous terpolymer and its application in cellulase immobilization
journal, June 2014

IMMOBILIZATION OF CELLULASES ON AMINE AND ALDEHYDE FUNCTIONALIZED Fe ₂ O ₃ MAGNETIC NANOPARTICLES
journal, May 2012

Study of cellulolytic enzyme immobilization on copolymers of N-vinylformamide
journal, October 2015

Enzyme Immobilization: The Quest for Optimum Performance
journal, June 2007

Encapsulation of cellulase with mesoporous silica (SBA-15)
journal, December 2008

Fuel Ethanol from Cellulosic Biomass
journal, March 1991

Improving the stability of cellulase by immobilization on modified polyvinyl alcohol coated chitosan beads
journal, April 2007

Enzyme Immobilization in Biotechnology
journal, November 2008

Structure of 3-aminopropyl triethoxy silane on silicon oxide
journal, November 1991

Immobilization of cellulase on magnetoresponsive graphene nano-supports
journal, June 2013

Bioethanol from lignocellulosics: Status and perspectives in Canada
journal, July 2010

Cellulase immobilized by sol–gel entrapment for efficient hydrolysis of cellulose
journal, October 2012

A novel method to prepare chitosan powder and its application in cellulase immobilization
journal, January 2006

Size selective protein adsorption on thiol-functionalised SBA-15 mesoporous molecular sieve
journal, January 2001

Immobilization of Aspergillus nidulans SU04 cellulase on modified activated carbon: Sorption and kinetic studies
journal, July 2011

Enzyme immobilization in MCM-41 molecular sieve
journal, December 1996

Sorption and immobilization of cellulase on silicate clay minerals
journal, October 2005

Active biocatalysts based on Candida rugosa lipase immobilized in vesicular silica
journal, June 2012

Functionalized Mesoporous Silica with Very Large Pores for Cellulase Immobilization
journal, April 2010

Cross-Linked Enzyme Aggregates as Industrial Biocatalysts
journal, January 2011

Exploring novel ultrafine Eri silk bioscaffold for enzyme stabilisation in cellobiose hydrolysis
journal, October 2013

Increase in stability of cellulase immobilized on functionalized magnetic nanospheres
journal, February 2015

Enzymes supported on ordered mesoporous solids: a special case of an inorganic–organic hybrid
journal, January 2005

Ordered Mesoporous Silica with Large Cage-Like Pores: Structural Identification and Pore Connectivity Design by Controlling the Synthesis Temperature and Time
journal, January 2003

Characterization and immobilization of liposome-bound cellulase for hydrolysis of insoluble cellulose
journal, May 2007

Cellulase immobilized mesoporous silica nanocatalysts for efficient cellulose-to-glucose conversion
journal, January 2011

Covalent Immobilization of Cellulases onto a Water-Soluble–Insoluble Reversible Polymer
journal, January 2012

Activity of glucose oxidase entrapped in mesoporous gels
journal, January 2005

Comparative Life-Cycle Assessments for Biomass-to-Ethanol Production from Different Regional Feedstocks
journal, January 2005

Improving Adsorbent Properties of Cage-like Ordered Amine Functionalized Mesoporous Silica with Very Large Pores for Bioadsorption
journal, June 2009

Immobilization of cellulase enzyme on functionalized multiwall carbon nanotubes
journal, September 2014

Outlook for cellulase improvement: Screening and selection strategies
journal, September 2006

Retention of Enzymatic Activity of α-Amylase in the Reductive Synthesis of Gold Nanoparticles
journal, May 2007

Suitability of magnetic nanoparticle immobilised cellulases in enhancing enzymatic saccharification of pretreated hemp biomass
journal, January 2014

Cellulase assisted synthesis of nano-silver and gold: Application as immobilization matrix for biocatalysis
journal, June 2015

Plant genetic engineering for biofuel production: towards affordable cellulosic ethanol
journal, June 2008

Fabrication of graphene oxide decorated with Fe3O4@SiO2 for immobilization of cellulase
journal, January 2015

Characterization of functionalized nanoporous supports for protein confinement
journal, October 2006

Synthesis and Bio-adsorptive Properties of Large-Pore Periodic Mesoporous Organosilica Rods
journal, November 2005

Physicochemical and catalytic properties of acylase I from aspergillus melleus immobilized on amino- and carbonyl-grafted stöber silica
journal, January 2018

Magnetic Recovery of Cellulase from Cellulose Substrates with Bare Iron Oxide Nanoparticles
journal, January 2019

Inulinase immobilization on polyethylene glycol/polypyrrole multiwall carbon nanotubes producing a catalyst with enhanced thermal and operational stability
journal, July 2019

Immobilization of alcohol dehydrogenase on ceramic silicon carbide membranes for enzymatic CH ₃ OH production: ADH immobilization on silicon carbide
journal, May 2018

Fabrication of iron oxide nanoparticles, and green catalytic application of an immobilized novel iron Schiff on wood cellulose
journal, December 2017

Continuous flow biocatalysis
journal, January 2018

QCM-D and NanoTweezer measurements to characterize the effect of soil cellulase on the deposition of PEG-coated TiO ₂ nanoparticles in model subsurface environments
journal, January 2018

Optical and structural properties of curcuminoids extracted from Curcuma longa L. for hybrid white light diode
journal, October 2018

A study on the enzymatic properties and reuse of cellulase immobilized with carbon nanotubes and sodium alginate
journal, July 2019