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Title: Recalcitrance and structural analysis by water-only flowthrough pretreatment of 13C enriched corn stover stem

Here, this study presents high temperature water-only continuous flowthrough pretreatment coupled with nuclear magnetic resonance (NMR) as a promising analytical tool to examine the plant cell wall, to understand its recalcitrance (i.e., cell wall resistance to deconstruction), and to probe the chemistry occurring during batch pretreatment of biomass. 13C-enriched corn stover stems were pretreated at 170 °C for 60 min with a hot-water flow rate of 20 mL/min to control fractionation of the cell wall. This approach helped elucidate the nature of plant cell wall chemical recalcitrance and biomass pretreatment chemistry by tracking cell wall fragmentation as a function of time. Fractions of the reactor effluent were collected in a time-resolved fashion and characterized by various NMR techniques to determine the degree and sequence of fragments released, as well as, the chemical composition, molecular structure, and relative molecular weight of those released fragments.
Authors:
 [1] ;  [2] ;  [3] ;  [4] ;  [5] ;  [6]
  1. Washington Univ., St. Louis, MO (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  2. The Univ. of British Columbia, Vancouver (Canada); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  3. Georgia Institute of Technology, Atlanta, GA (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  4. Univ. of California, Riverside, CA (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  5. Washington Univ., St. Louis, MO (United States)
  6. Univ. of Tennessee, Knoxville, TN (United States); Georgia Institute of Technology, Atlanta, GA (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Publication Date:
OSTI Identifier:
1266001
Grant/Contract Number:
AC05-00OR22725
Type:
Accepted Manuscript
Journal Name:
Bioresource Technology
Additional Journal Information:
Journal Volume: 197; Journal ID: ISSN 0960-8524
Publisher:
Elsevier
Research Org:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC)
Sponsoring Org:
USDOE Office of Science (SC)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES recalcitrance; corn stover; hot-water flowthrough pretreatment; nuclear magnetic resonance