skip to main content

DOE PAGESDOE PAGES

Title: A comprehensive benchmarking study of protocols and sequencing platforms for 16S rRNA community profiling

In the last 5 years, the rapid pace of innovations and improvements in sequencing technologies has completely changed the landscape of metagenomic and metagenetic experiments. Therefore, it is critical to benchmark the various methodologies for interrogating the composition of microbial communities, so that we can assess their strengths and limitations. Here, the most common phylogenetic marker for microbial community diversity studies is the 16S ribosomal RNA gene and in the last 10 years the field has moved from sequencing a small number of amplicons and samples to more complex studies where thousands of samples and multiple different gene regions are interrogated.
Authors:
 [1] ;  [2] ;  [3] ;  [4] ;  [4] ;  [3] ;  [3] ;  [3] ;  [5] ;  [3]
  1. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  2. Dartmouth College, Hanover, NH (United States)
  3. Univ. of Liverpool, Liverpool (United Kingdom)
  4. Univ. of Glasgow, Glasgow (United Kingdom)
  5. Univ. of Warwick, Warwick (United Kingdom)
Publication Date:
OSTI Identifier:
1265994
Grant/Contract Number:
AC05-00OR22725
Type:
Accepted Manuscript
Journal Name:
BMC Genomics
Additional Journal Information:
Journal Volume: 17; Journal Issue: 1; Journal ID: ISSN 1471-2164
Publisher:
Springer
Research Org:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Sponsoring Org:
ORNL LDRD Director's R&D; USDOE
Country of Publication:
United States
Language:
English