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Title: Ebolavirus comparative genomics

The 2014 Ebola outbreak in West Africa is the largest documented for this virus. We examine the dynamics of this genome, comparing more than one hundred currently available ebolavirus genomes to each other and to other viral genomes. Based on oligomer frequency analysis, the family Filoviridae forms a distinct group from all other sequenced viral genomes. All filovirus genomes sequenced to date encode proteins with similar functions and gene order, although there is considerable divergence in sequences between the three genera Ebolavirus, Cuevavirus, and Marburgvirus within the family Filoviridae. Whereas all ebolavirus genomes are quite similar (multiple sequences of the same strain are often identical), variation is most common in the intergenic regions and within specific areas of the genes encoding the glycoprotein (GP), nucleoprotein (NP), and polymerase (L). We predict regions that could contain epitope-binding sites, which might be good vaccine targets. In conclusion, this information, combined with glycosylation sites and experimentally determined epitopes, can identify the most promising regions for the development of therapeutic strategies.
Authors:
 [1] ;  [2] ;  [3] ;  [3] ;  [3] ;  [4] ;  [3] ;  [2] ;  [5] ;  [6] ;  [6] ;  [7] ;  [8] ;  [9] ;  [4]
  1. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division; Univ. of Tennessee, Knoxville, TN (United States). Joint Inst. for Computational Sciences
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Computer Science and Mathematics Division
  3. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division
  4. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division; Univ. of Tennessee, Knoxville, TN (United States). UT-ORNL Graduate School of Genome Science and Technology
  5. Technical Univ. of Denmark, Lyngby (Denmark). Center for Biological Sequence Analysis; Univ. Nacional de San Martin, Buenos Aires (Argentina)
  6. Technical Univ. of Denmark, Lyngby (Denmark). Center for Biological Sequence Analysis
  7. Booze Allen Hamilton, McLean, VA (United States)
  8. Technical Univ. of Denmark, Lyngby (Denmark). Center for Biological Sequence Analysis; Chr. Hansen A/S, Horsholm (Denmark). Assays, Cultures and Enzymes Division
  9. Microbiology and Genomics Consultants, Zotzenheim (Germany)
Publication Date:
OSTI Identifier:
1265422
Grant/Contract Number:
AC05-00OR22725
Type:
Accepted Manuscript
Journal Name:
FEMS Microbiology Reviews
Additional Journal Information:
Journal Volume: 39; Journal Issue: 5; Journal ID: ISSN 1574-6976
Publisher:
Federation of European Microbiological Societies
Research Org:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES Ebola Virus; comparative genomics; viral genomes; epitope prediction; Ebola virus disease (EVD); Filovirus