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Title: A Centrifugal Microfluidic Platform That Separates Whole Blood Samples into Multiple Removable Fractions Due to Several Discrete but Continuous Density Gradient Sections

We present a miniaturized centrifugal platform that uses density centrifugation for separation and analysis of biological components in small volume samples (~5 μL). We demonstrate the ability to enrich leukocytes for on-disk visualization via microscopy, as well as recovery of viable cells from each of the gradient partitions. In addition, we simplified the traditional Modified Wright-Giemsa staining by decreasing the time, volume, and expertise involved in the procedure. From a whole blood sample, we were able to extract 95.15% of leukocytes while excluding 99.8% of red blood cells. Ultimately, this platform has great potential in both medical diagnostics and research applications as it offers a simpler, automated, and inexpensive method for biological sample separation, analysis, and downstream culturing.
Authors:
 [1] ;  [2] ;  [1]
  1. Sandia National Lab. (SNL-CA), Livermore, CA (United States); Univ. of Texas Medical Branch, Galveston, TX (United States)
  2. Univ. of Texas Medical Branch, Galveston, TX (United States)
Publication Date:
OSTI Identifier:
1261101
Grant/Contract Number:
AC04-94AL85000; R01AI0988530
Type:
Accepted Manuscript
Journal Name:
PLoS ONE
Additional Journal Information:
Journal Volume: 11; Journal Issue: 4; Journal ID: ISSN 1932-6203
Publisher:
Public Library of Science
Research Org:
Sandia National Laboratories (SNL-CA), Livermore, CA (United States)
Sponsoring Org:
USDOE; National Nuclear Security Administration (NNSA)
Country of Publication:
United States
Language:
English
Subject:
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