An innovative platform for quick and flexible joining of assorted DNA fragments
Abstract
Successful synthetic biology efforts rely on conceptual and experimental designs in combination with testing of multi-gene constructs. Despite recent progresses, several limitations still hinder the ability to flexibly assemble and collectively share different types of DNA segments. We describe an advanced system for joining DNA fragments from a universal library that automatically maintains open reading frames (ORFs) and does not require linkers, adaptors, sequence homology, amplification or mutation (domestication) of fragments in order to work properly. Moreover, we find that this system, which is enhanced by a unique buffer formulation, provides unforeseen capabilities for testing, and sharing, complex multi-gene circuitry assembled from different DNA fragments.
- Authors:
-
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1324162
- Alternate Identifier(s):
- OSTI ID: 1259839
- Grant/Contract Number:
- AC05-00OR22725; SC0008834
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Scientific Reports
- Additional Journal Information:
- Journal Volume: 6; Journal ID: ISSN 2045-2322
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
De Paoli, Henrique Cestari, Tuskan, Gerald A., and Yang, Xiaohan. An innovative platform for quick and flexible joining of assorted DNA fragments. United States: N. p., 2016.
Web. doi:10.1038/srep19278.
De Paoli, Henrique Cestari, Tuskan, Gerald A., & Yang, Xiaohan. An innovative platform for quick and flexible joining of assorted DNA fragments. United States. https://doi.org/10.1038/srep19278
De Paoli, Henrique Cestari, Tuskan, Gerald A., and Yang, Xiaohan. Wed .
"An innovative platform for quick and flexible joining of assorted DNA fragments". United States. https://doi.org/10.1038/srep19278. https://www.osti.gov/servlets/purl/1324162.
@article{osti_1324162,
title = {An innovative platform for quick and flexible joining of assorted DNA fragments},
author = {De Paoli, Henrique Cestari and Tuskan, Gerald A. and Yang, Xiaohan},
abstractNote = {Successful synthetic biology efforts rely on conceptual and experimental designs in combination with testing of multi-gene constructs. Despite recent progresses, several limitations still hinder the ability to flexibly assemble and collectively share different types of DNA segments. We describe an advanced system for joining DNA fragments from a universal library that automatically maintains open reading frames (ORFs) and does not require linkers, adaptors, sequence homology, amplification or mutation (domestication) of fragments in order to work properly. Moreover, we find that this system, which is enhanced by a unique buffer formulation, provides unforeseen capabilities for testing, and sharing, complex multi-gene circuitry assembled from different DNA fragments.},
doi = {10.1038/srep19278},
journal = {Scientific Reports},
number = ,
volume = 6,
place = {United States},
year = {Wed Jan 13 00:00:00 EST 2016},
month = {Wed Jan 13 00:00:00 EST 2016}
}
Web of Science
Figures / Tables:
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Figures / Tables found in this record: