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This content will become publicly available on December 18, 2016

Title: Structure and membrane remodeling activity of ESCRT-III helical polymers

The endosomal sorting complexes required for transport (ESCRT) proteins mediate fundamental membrane remodeling events that require stabilizing negative membrane curvature. These include endosomal intralumenal vesicle formation, HIV budding, nuclear envelope closure, and cytokinetic abscission. ESCRT-III subunits perform key roles in these processes by changing conformation and polymerizing into membrane-remodeling filaments. Here, we report the 4 angstrom resolution cryogenic electron microscopy reconstruction of a one-start, double-stranded helical copolymer composed of two different human ESCRT-III subunits, charged multivesicular body protein 1B (CHMP1B) and increased sodium tolerance 1 (IST1). The inner strand comprises “open” CHMP1B subunits that interlock in an elaborate domain-swapped architecture and is encircled by an outer strand of “closed” IST1 subunits. Unlike other ESCRT-III proteins, CHMP1B and IST1 polymers form external coats on positively curved membranes in vitro and in vivo. In conclusion, our analysis suggests how common ESCRT-III filament architectures could stabilize different degrees and directions of membrane curvature.
Authors:
 [1] ;  [2] ;  [3] ;  [2] ;  [1] ;  [2] ;  [1] ;  [4] ;  [5] ;  [1] ;  [2] ;  [3]
  1. Univ. of Utah, Salt Lake City, UT (United States)
  2. Washington Univ. School of Medicine, St. Louis, MO (United States)
  3. Univ. of Utah, Salt Lake City, UT (United States); Univ. of California, San Francisco, CA (United States)
  4. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  5. FEI Company, Hillsboro, OR (United States)
Publication Date:
OSTI Identifier:
1257996
Grant/Contract Number:
AC02-05CH11231; DGE-1143954; 2P50GM082545-06
Type:
Accepted Manuscript
Journal Name:
Science
Additional Journal Information:
Journal Volume: 350; Journal Issue: 6267; Journal ID: ISSN 0036-8075
Publisher:
AAAS
Research Org:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES