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Title: Reversible Aptamer-Au Plasmon Rulers for Secreted Single Molecules

Plasmon rulers, consisting of pairs of gold nanoparticles, allow single-molecule analysis without photobleaching or blinking; however, current plasmon rulers are irreversible, restricting detection to only single events. Here, we present a reversible plasmon ruler, comprised of coupled gold nanoparticles linked by a single aptamer, capable of binding individual secreted molecules with high specificity. We show that the binding of target secreted molecules to the reversible plasmon ruler is characterized by single-molecule sensitivity, high specificity, and reversibility. Lastly, such reversible plasmon rulers should enable dynamic and adaptive live-cell measurement of secreted single molecules in their local microenvironment.
Authors:
 [1] ;  [2] ;  [3] ;  [2] ;  [2] ;  [2] ;  [4] ;  [5] ;  [4]
  1. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Univ. of Michigan, Ann Arbor, MI (United States). Biointerfaces Institute
  2. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Material Science Division; Univ. of California, Berkeley, CA (United States). Dept. of Chemistry and Materials Science & Engineering
  3. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Life Sciences Division; Indian Institute of Science, Bangalore (India). Dept. of Molecular Reproduction Development and Genetics
  4. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Life Sciences Division
  5. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Material Science Division; Univ. of California, Berkeley, CA (United States). Kavli Energy NanoScience Institute
Publication Date:
OSTI Identifier:
1257849
Grant/Contract Number:
AC02-05CH1123; R01CA057621; R01CA140663; R37CA064786; U54CA112970; KG111229; F32 EB013972; W81XWH0810736
Type:
Accepted Manuscript
Journal Name:
Nano Letters
Additional Journal Information:
Journal Volume: 15; Journal Issue: 7; Journal ID: ISSN 1530-6984
Publisher:
American Chemical Society
Research Org:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Country of Publication:
United States
Language:
English
Subject:
77 NANOSCIENCE AND NANOTECHNOLOGY gold nanoparticle; imaging; plasmon; secreted molecule; single-molecule