Efficient linear phase contrast in scanning transmission electron microscopy with matched illumination and detector interferometry
Abstract
The ability to image light elements in soft matter at atomic resolution enables unprecedented insight into the structure and properties of molecular heterostructures and beam-sensitive nanomaterials. In this study, we introduce a scanning transmission electron microscopy technique combining a pre-specimen phase plate designed to produce a probe with structured phase with a high-speed direct electron detector to generate nearly linear contrast images with high efficiency. We demonstrate this method by using both experiment and simulation to simultaneously image the atomic-scale structure of weakly scattering amorphous carbon and strongly scattering gold nanoparticles. Our method demonstrates strong contrast for both materials, making it a promising candidate for structural determination of het erogeneous soft/hard matter samples even at low electron doses comparable to traditional phase-contrast transmission electron microscopy. Simulated images demonstrate the extension of this technique to the challenging problem of structural determination of biological material at the surface of inorganic crystals.
- Authors:
-
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Univ. of Oregon, Eugene, OR (United States)
- Gatan Inc., Pleasanton, CA (United States)
- Ulm Univ., Ulm (Germany)
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1255550
- Alternate Identifier(s):
- OSTI ID: 1379111
- Grant/Contract Number:
- AC02-05CH11231; SC0010466
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Nature Communications
- Additional Journal Information:
- Journal Volume: 7; Journal ID: ISSN 2041-1723
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 77 NANOSCIENCE AND NANOTECHNOLOGY
Citation Formats
Ophus, Colin, Ciston, Jim, Pierce, Jordan, Harvey, Tyler R., Chess, Jordan, McMorran, Benjamin J., Czarnik, Cory, Rose, Harald H., and Ercius, Peter. Efficient linear phase contrast in scanning transmission electron microscopy with matched illumination and detector interferometry. United States: N. p., 2016.
Web. doi:10.1038/ncomms10719.
Ophus, Colin, Ciston, Jim, Pierce, Jordan, Harvey, Tyler R., Chess, Jordan, McMorran, Benjamin J., Czarnik, Cory, Rose, Harald H., & Ercius, Peter. Efficient linear phase contrast in scanning transmission electron microscopy with matched illumination and detector interferometry. United States. https://doi.org/10.1038/ncomms10719
Ophus, Colin, Ciston, Jim, Pierce, Jordan, Harvey, Tyler R., Chess, Jordan, McMorran, Benjamin J., Czarnik, Cory, Rose, Harald H., and Ercius, Peter. Mon .
"Efficient linear phase contrast in scanning transmission electron microscopy with matched illumination and detector interferometry". United States. https://doi.org/10.1038/ncomms10719. https://www.osti.gov/servlets/purl/1255550.
@article{osti_1255550,
title = {Efficient linear phase contrast in scanning transmission electron microscopy with matched illumination and detector interferometry},
author = {Ophus, Colin and Ciston, Jim and Pierce, Jordan and Harvey, Tyler R. and Chess, Jordan and McMorran, Benjamin J. and Czarnik, Cory and Rose, Harald H. and Ercius, Peter},
abstractNote = {The ability to image light elements in soft matter at atomic resolution enables unprecedented insight into the structure and properties of molecular heterostructures and beam-sensitive nanomaterials. In this study, we introduce a scanning transmission electron microscopy technique combining a pre-specimen phase plate designed to produce a probe with structured phase with a high-speed direct electron detector to generate nearly linear contrast images with high efficiency. We demonstrate this method by using both experiment and simulation to simultaneously image the atomic-scale structure of weakly scattering amorphous carbon and strongly scattering gold nanoparticles. Our method demonstrates strong contrast for both materials, making it a promising candidate for structural determination of het erogeneous soft/hard matter samples even at low electron doses comparable to traditional phase-contrast transmission electron microscopy. Simulated images demonstrate the extension of this technique to the challenging problem of structural determination of biological material at the surface of inorganic crystals.},
doi = {10.1038/ncomms10719},
journal = {Nature Communications},
number = ,
volume = 7,
place = {United States},
year = {Mon Feb 29 00:00:00 EST 2016},
month = {Mon Feb 29 00:00:00 EST 2016}
}
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