DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Selection and molecular characterization of cellulolytic–xylanolytic fungi from surface soil-biomass mixtures from Black Belt sites

Abstract

Plant biomass is an abundant renewable natural resource that can be transformed into chemical feedstocks. Enzymes used in saccharification of lignocellulosic biomass is a major part of biofuel production costs. A cocktail of cellulolytic and xylanolytic enzymes are required for optimal saccharification of biomass. Accordingly, thirty-two fungal pure cultures were obtained from surface soil-biomass mixtures collected from Black Belt sites in Alabama by culturing on lignocellulosic biomass medium. The fungal strains were screened for the coproduction of cellulolytic and xylanolytic enzymes. Strains that displayed promising levels of cellulolytic and xylanolytic enzymes were characterized by molecular analysis of DNA sequences from the large subunit and internal transcribed spacer (ITS) of their ribosomal RNA gene. Nucleotide sequence analysis revealed that two most promising isolates FS22A and FS5A were most similar to Penicillium janthinellum and Trichoderma virens. Production dynamics of cellulolytic and xylanolytic enzymes from these two strains were explored in submerged fermentation. Volumetric productivity after 120h incubation was 121.08 units/L/h and 348 units/L/h for the filter paper cellulase and xylanase of strain FS22A, and 90.83 units/L/h and 359 units/L/h, respectively for strain FS5A. Assays with 10 times dilution of enzymes revealed that the activities were much higher than that observed in themore » crude culture supernatant. Additionally, both FS22A and FS5A also produced amylase in lignocellulose medium. The enzyme profiles of these strains and their activities suggest potential applications in cost effective biomass conversion and biodegradation.« less

Authors:
; ; ; ; ; ;
Publication Date:
Research Org.:
Auburn University at Montgomery
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office
OSTI Identifier:
1255167
Alternate Identifier(s):
OSTI ID: 1419495
Grant/Contract Number:  
EE0003132
Resource Type:
Published Article
Journal Name:
Microbiological Research
Additional Journal Information:
Journal Name: Microbiological Research Journal Volume: 175 Journal Issue: C; Journal ID: ISSN 0944-5013
Publisher:
Elsevier
Country of Publication:
Germany
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; cellulolytic enzymes; xylanolytic enzymes; lignocellulose biomass

Citation Formats

Okeke, Benedict C., Hall, Rosine W., Nanjundaswamy, Ananda, Thomson, M. Sue, Deravi, Yasaman, Sawyer, Leah, and Prescott, Andrew. Selection and molecular characterization of cellulolytic–xylanolytic fungi from surface soil-biomass mixtures from Black Belt sites. Germany: N. p., 2015. Web. doi:10.1016/j.micres.2015.03.001.
Okeke, Benedict C., Hall, Rosine W., Nanjundaswamy, Ananda, Thomson, M. Sue, Deravi, Yasaman, Sawyer, Leah, & Prescott, Andrew. Selection and molecular characterization of cellulolytic–xylanolytic fungi from surface soil-biomass mixtures from Black Belt sites. Germany. https://doi.org/10.1016/j.micres.2015.03.001
Okeke, Benedict C., Hall, Rosine W., Nanjundaswamy, Ananda, Thomson, M. Sue, Deravi, Yasaman, Sawyer, Leah, and Prescott, Andrew. Mon . "Selection and molecular characterization of cellulolytic–xylanolytic fungi from surface soil-biomass mixtures from Black Belt sites". Germany. https://doi.org/10.1016/j.micres.2015.03.001.
@article{osti_1255167,
title = {Selection and molecular characterization of cellulolytic–xylanolytic fungi from surface soil-biomass mixtures from Black Belt sites},
author = {Okeke, Benedict C. and Hall, Rosine W. and Nanjundaswamy, Ananda and Thomson, M. Sue and Deravi, Yasaman and Sawyer, Leah and Prescott, Andrew},
abstractNote = {Plant biomass is an abundant renewable natural resource that can be transformed into chemical feedstocks. Enzymes used in saccharification of lignocellulosic biomass is a major part of biofuel production costs. A cocktail of cellulolytic and xylanolytic enzymes are required for optimal saccharification of biomass. Accordingly, thirty-two fungal pure cultures were obtained from surface soil-biomass mixtures collected from Black Belt sites in Alabama by culturing on lignocellulosic biomass medium. The fungal strains were screened for the coproduction of cellulolytic and xylanolytic enzymes. Strains that displayed promising levels of cellulolytic and xylanolytic enzymes were characterized by molecular analysis of DNA sequences from the large subunit and internal transcribed spacer (ITS) of their ribosomal RNA gene. Nucleotide sequence analysis revealed that two most promising isolates FS22A and FS5A were most similar to Penicillium janthinellum and Trichoderma virens. Production dynamics of cellulolytic and xylanolytic enzymes from these two strains were explored in submerged fermentation. Volumetric productivity after 120h incubation was 121.08 units/L/h and 348 units/L/h for the filter paper cellulase and xylanase of strain FS22A, and 90.83 units/L/h and 359 units/L/h, respectively for strain FS5A. Assays with 10 times dilution of enzymes revealed that the activities were much higher than that observed in the crude culture supernatant. Additionally, both FS22A and FS5A also produced amylase in lignocellulose medium. The enzyme profiles of these strains and their activities suggest potential applications in cost effective biomass conversion and biodegradation.},
doi = {10.1016/j.micres.2015.03.001},
journal = {Microbiological Research},
number = C,
volume = 175,
place = {Germany},
year = {Mon Jun 01 00:00:00 EDT 2015},
month = {Mon Jun 01 00:00:00 EDT 2015}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1016/j.micres.2015.03.001

Citation Metrics:
Cited by: 11 works
Citation information provided by
Web of Science

Save / Share:

Works referencing / citing this record:

Talaromyces borbonicus , sp. nov., a novel fungus from biodegraded Arundo donax with potential abilities in lignocellulose conversion
journal, March 2018