A squalene synthase-like enzyme initiates production of tetraterpenoid hydrocarbons in Botryococcus braunii Race L
Abstract
Here, the green microalga Botryococcus braunii is considered a promising biofuel feedstock producer due to its prodigious accumulation of hydrocarbon oils that can be converted into fuels. B. braunii Race L produces the C40 tetraterpenoid hydrocarbon lycopadiene via an uncharacterized biosynthetic pathway. Structural similarities suggest this pathway follows a biosynthetic mechanism analogous to that of C30 squalene. Confirming this hypothesis, the current study identifies C20 geranylgeranyl diphosphate (GGPP) as a precursor for lycopaoctaene biosynthesis, the first committed intermediate in the production of lycopadiene. Two squalene synthase (SS)-like complementary DNAs are identified in race L with one encoding a true SS and the other encoding an enzyme with lycopaoctaene synthase (LOS) activity. Interestingly, LOS uses alternative C15 and C20 prenyl diphosphate substrates to produce combinatorial hybrid hydrocarbons, but almost exclusively uses GGPP in vivo. In conclusion, this discovery highlights how SS enzyme diversification results in the production of specialized tetraterpenoid oils in race L of B. braunii.
- Authors:
-
- Texas A&M Univ., College Station, TX (United States). Dept. of Biochemistry and Biophysics
- Texas A&M Univ., College Station, TX (United States). Dept. of Biochemistry and Biophysics; Texas A&M Univ., College Station, TX (United States). Biomolecular NMR Laboratory
- Univ. of Tokyo (Japan); Japan Science and Technology Agency-Core Research for Evolutional Science and Technology (CREST), Tokyo (Japan)
- Univ. of Arizona, Tucson, AZ (United States)
- Univ. of Arizona, Tucson, AZ (United States); Chinese Academy of Sciences (CAS), Beijing (China)
- Publication Date:
- Research Org.:
- Texas A&M AgriLife Research, College Station, TX (United States)
- Sponsoring Org.:
- USDOE Advanced Research Projects Agency - Energy (ARPA-E); USDOE Office of Energy Efficiency and Renewable Energy (EERE)
- Contributing Org.:
- Texas A&M AgriLife Research
- OSTI Identifier:
- 1255082
- Grant/Contract Number:
- AR0000203; EE0003046l; NSF-EFRI-PSBR 1240478
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Nature Communications
- Additional Journal Information:
- Journal Volume: 7; Journal ID: ISSN 2041-1723
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; biological sciences; biochemistry; chemical biology; plant sciences
Citation Formats
Thapa, Hem R., Naik, Mandar T., Okada, Shigeru, Takada, Kentaro, Molnar, Istvan, Xu, Yuquan, and Devarenne, Timothy P. A squalene synthase-like enzyme initiates production of tetraterpenoid hydrocarbons in Botryococcus braunii Race L. United States: N. p., 2016.
Web. doi:10.1038/ncomms11198.
Thapa, Hem R., Naik, Mandar T., Okada, Shigeru, Takada, Kentaro, Molnar, Istvan, Xu, Yuquan, & Devarenne, Timothy P. A squalene synthase-like enzyme initiates production of tetraterpenoid hydrocarbons in Botryococcus braunii Race L. United States. https://doi.org/10.1038/ncomms11198
Thapa, Hem R., Naik, Mandar T., Okada, Shigeru, Takada, Kentaro, Molnar, Istvan, Xu, Yuquan, and Devarenne, Timothy P. Wed .
"A squalene synthase-like enzyme initiates production of tetraterpenoid hydrocarbons in Botryococcus braunii Race L". United States. https://doi.org/10.1038/ncomms11198. https://www.osti.gov/servlets/purl/1255082.
@article{osti_1255082,
title = {A squalene synthase-like enzyme initiates production of tetraterpenoid hydrocarbons in Botryococcus braunii Race L},
author = {Thapa, Hem R. and Naik, Mandar T. and Okada, Shigeru and Takada, Kentaro and Molnar, Istvan and Xu, Yuquan and Devarenne, Timothy P.},
abstractNote = {Here, the green microalga Botryococcus braunii is considered a promising biofuel feedstock producer due to its prodigious accumulation of hydrocarbon oils that can be converted into fuels. B. braunii Race L produces the C40 tetraterpenoid hydrocarbon lycopadiene via an uncharacterized biosynthetic pathway. Structural similarities suggest this pathway follows a biosynthetic mechanism analogous to that of C30 squalene. Confirming this hypothesis, the current study identifies C20 geranylgeranyl diphosphate (GGPP) as a precursor for lycopaoctaene biosynthesis, the first committed intermediate in the production of lycopadiene. Two squalene synthase (SS)-like complementary DNAs are identified in race L with one encoding a true SS and the other encoding an enzyme with lycopaoctaene synthase (LOS) activity. Interestingly, LOS uses alternative C15 and C20 prenyl diphosphate substrates to produce combinatorial hybrid hydrocarbons, but almost exclusively uses GGPP in vivo. In conclusion, this discovery highlights how SS enzyme diversification results in the production of specialized tetraterpenoid oils in race L of B. braunii.},
doi = {10.1038/ncomms11198},
journal = {Nature Communications},
number = ,
volume = 7,
place = {United States},
year = {Wed Apr 06 00:00:00 EDT 2016},
month = {Wed Apr 06 00:00:00 EDT 2016}
}
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Works referenced in this record:
Gene clustering in plant specialized metabolism
journal, April 2014
- Nützmann, Hans-Wilhelm; Osbourn, Anne
- Current Opinion in Biotechnology, Vol. 26
Characterization of the GGPP synthase gene family in Arabidopsis thaliana
journal, June 2013
- Beck, Gilles; Coman, Diana; Herren, Edgar
- Plant Molecular Biology, Vol. 82, Issue 4-5
Botryococcus braunii : A Renewable Source of Hydrocarbons and Other Chemicals
journal, January 2002
- Banerjee, Anirban; Sharma, Rohit; Chisti, Yusuf
- Critical Reviews in Biotechnology, Vol. 22, Issue 3
Raman Spectroscopy Analysis of Botryococcene Hydrocarbons from the Green Microalga Botryococcus braunii
journal, August 2010
- Weiss, Taylor L.; Chun, Hye Jin; Okada, Shigeru
- Journal of Biological Chemistry, Vol. 285, Issue 42
Efficient diterpene production in yeast by engineering Erg20p into a geranylgeranyl diphosphate synthase
journal, January 2015
- Ignea, Codruta; Trikka, Fotini A.; Nikolaidis, Alexandros K.
- Metabolic Engineering, Vol. 27
Efficient diterpene production in yeast by engineering Erg20p into a geranylgeranyl diphosphate synthase
journal, January 2015
- Ignea, Codruta; Trikka, Fotini A.; Nikolaidis, Alexandros K.
- Metabolic Engineering, Vol. 27
Botryococcane in a new class of Australian non-marine crude oils
journal, March 1986
- McKirdy, D. M.; Cox, R. E.; Volkman, J. K.
- Nature, Vol. 320, Issue 6057
Metabolon formation and metabolic channeling in the biosynthesis of plant natural products
journal, June 2005
- Jørgensen, Kirsten; Rasmussen, Anne Vinther; Morant, Marc
- Current Opinion in Plant Biology, Vol. 8, Issue 3
Crystal Structure of Human Squalene Synthase: A KEY ENZYME IN CHOLESTEROL BIOSYNTHESIS
journal, July 2000
- Pandit, Jayvardhan; Danley, Dennis E.; Schulte, Gayle K.
- Journal of Biological Chemistry, Vol. 275, Issue 39
Sesquarterpenes (C 35 Terpenes) Biosynthesized via the Cyclization of a Linear C 35 Isoprenoid by a Tetraprenyl-β-curcumene Synthase and a Tetraprenyl-β-curcumene Cyclase: Identification of a New Terpene Cyclase
journal, June 2011
- Sato, Tsutomu; Yoshida, Satoru; Hoshino, Hiroko
- Journal of the American Chemical Society, Vol. 133, Issue 25
Characterization of botryococcene synthase enzyme activity, a squalene synthase-like activity from the green microalga Botryococcus braunii, Race B
journal, February 2004
- Okada, Shigeru; Devarenne, Timothy P.; Murakami, Masahiro
- Archives of Biochemistry and Biophysics, Vol. 422, Issue 1
Steady-State Kinetic Characterization of Sesquiterpene Synthases by Gas Chromatography–Mass Spectroscopy
book, January 2012
- Garrett, Steven R.; Morris, Richard J.; O'Maille, Paul E.
- Natural Product Biosynthesis by Microorganisms and Plants, Part A
Sesquarterpenes (C 35 Terpenes) Biosynthesized via the Cyclization of a Linear C 35 Isoprenoid by a Tetraprenyl-β-curcumene Synthase and a Tetraprenyl-β-curcumene Cyclase: Identification of a New Terpene Cyclase
journal, June 2011
- Sato, Tsutomu; Yoshida, Satoru; Hoshino, Hiroko
- Journal of the American Chemical Society, Vol. 133, Issue 25
Chemotaxonomic evidence for the similarity between Botryococcus brauniiL race and Botryococcus neglectus
journal, March 1997
- Metzger, P.; Pouet, Y.; Summons, R.
- Phytochemistry, Vol. 44, Issue 6
Zaragozic acids: a family of fungal metabolites that are picomolar competitive inhibitors of squalene synthase.
journal, January 1993
- Bergstrom, J. D.; Kurtz, M. M.; Rew, D. J.
- Proceedings of the National Academy of Sciences, Vol. 90, Issue 1
Triterpene hydrocarbon production engineered into a metabolically versatile host- Rhodobacter capsulatus : Triterpene Hydrocarbon Production Engineered
journal, May 2015
- Khan, Nymul E.; Nybo, S. Eric; Chappell, Joe
- Biotechnology and Bioengineering, Vol. 112, Issue 8
Metabolon formation and metabolic channeling in the biosynthesis of plant natural products
journal, June 2005
- Jørgensen, Kirsten; Rasmussen, Anne Vinther; Morant, Marc
- Current Opinion in Plant Biology, Vol. 8, Issue 3
Biosynthesis of non-head-to-tail terpenes. Formation of 1'-1 and 1'-3 linkages
journal, March 1990
- Poulter, C. Dale
- Accounts of Chemical Research, Vol. 23, Issue 3
Recombinant Squalene Synthase. Synthesis of Non-Head-to-Tail Isoprenoids in the Absence of NADPH
journal, July 2002
- Jarstfer, Michael B.; Zhang, Dong-Lu; Poulter, C. Dale
- Journal of the American Chemical Society, Vol. 124, Issue 30
Production of C35 isoprenoids depends on H2 availability during cultivation of the hyperthermophile Methanococcus jannaschii
journal, October 2003
- Manquin, Brendan P.; Morgan, John A.; Ju, Jaeyeong
- Extremophiles, Vol. 8, Issue 1
Isolation and identification of “bacteriorhodopsin” and minor C40-carotenoids in Halobacterium cutirubrum
journal, August 1973
- Kushwaha, S. C.; Kates, M.
- Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, Vol. 316, Issue 2
Metabolic engineering for the production of hydrocarbon fuels
journal, June 2015
- Lee, Sang Yup; Kim, Hye Mi; Cheon, Seungwoo
- Current Opinion in Biotechnology, Vol. 33
Biosynthesis of the triterpenoids, botryococcenes and tetramethylsqualene in the B race of Botryococcus braunii via the non-mevalonate pathway
journal, September 2003
- Sato, Yohei; Ito, Yusai; Okada, Shigeru
- Tetrahedron Letters, Vol. 44, Issue 37
Biosynthesis of Squalene from Farnesyl Diphosphate in Bacteria: Three Steps Catalyzed by Three Enzymes
journal, April 2015
- Pan, Jian-Jung; Solbiati, Jose O.; Ramamoorthy, Gurusankar
- ACS Central Science, Vol. 1, Issue 2
Botryococcus braunii: a rich source for hydrocarbons and related ether lipids
journal, December 2004
- Metzger, P.; Largeau, C.
- Applied Microbiology and Biotechnology, Vol. 66, Issue 5
Inhibition of Mammalian Squalene Synthetase Activity by Zaragozic Acid A Is a Result of Competitive Inhibition Followed by Mechanism-based Irreversible Inactivation
journal, April 1995
- Lindsey, Saralyn; Harwood, H. James
- Journal of Biological Chemistry, Vol. 270, Issue 16
Inhibition of Mammalian Squalene Synthetase Activity by Zaragozic Acid A Is a Result of Competitive Inhibition Followed by Mechanism-based Irreversible Inactivation
journal, April 1995
- Lindsey, Saralyn; Harwood, H. James
- Journal of Biological Chemistry, Vol. 270, Issue 16
Possible algal origin of long chain odd n-alkanes in immature sediments as revealed by distributions and carbon isotope ratios
journal, December 1994
- Lichtfouse, Éric; Derenne, Sylvie; Mariotti, André
- Organic Geochemistry, Vol. 22, Issue 6
Lycopadiene, a tetraterpenoid hydrocarbon from new strains of the green alga Botryococcus braunii.
journal, January 1987
- Metzger, P.; Casadevall, E.
- Tetrahedron Letters, Vol. 28, Issue 34
Recombinant Squalene Synthase. Synthesis of Non-Head-to-Tail Isoprenoids in the Absence of NADPH
journal, July 2002
- Jarstfer, Michael B.; Zhang, Dong-Lu; Poulter, C. Dale
- Journal of the American Chemical Society, Vol. 124, Issue 30
First discovery of botryococcane in petroleum
journal, January 1980
- Moldowan, J. Michael; Seifert, Wolfgang K.
- Journal of the Chemical Society, Chemical Communications, Issue 19
Zaragozic acids: a family of fungal metabolites that are picomolar competitive inhibitors of squalene synthase.
journal, January 1993
- Bergstrom, J. D.; Kurtz, M. M.; Rew, D. J.
- Proceedings of the National Academy of Sciences, Vol. 90, Issue 1
ChemInform Abstract: Hopanoids. Part 2. Biohopanoids: A Novel Class of Bacterial Lipids
journal, August 2010
- Ourisson, G.; Rohmer, M.
- ChemInform, Vol. 24, Issue 1
Evolution of a Pathway to Novel Long-Chain Carotenoids
journal, February 2004
- Umeno, D.; Arnold, F. H.
- Journal of Bacteriology, Vol. 186, Issue 5
Possible algal origin of long chain odd n-alkanes in immature sediments as revealed by distributions and carbon isotope ratios
journal, December 1994
- Lichtfouse, Éric; Derenne, Sylvie; Mariotti, André
- Organic Geochemistry, Vol. 22, Issue 6
Genetic and biochemical analyses of the biosynthesis of the yellow carotenoid 4,4'-diaponeurosporene of Staphylococcus aureus.
journal, December 1994
- Wieland, B.; Feil, C.; Gloria-Maercker, E.
- Journal of Bacteriology, Vol. 176, Issue 24
Characterization of botryococcene synthase enzyme activity, a squalene synthase-like activity from the green microalga Botryococcus braunii, Race B
journal, February 2004
- Okada, Shigeru; Devarenne, Timothy P.; Murakami, Masahiro
- Archives of Biochemistry and Biophysics, Vol. 422, Issue 1
Bio-crude transcriptomics: Gene discovery and metabolic network reconstruction for the biosynthesis of the terpenome of the hydrocarbon oil-producing green alga, Botryococcus braunii race B (Showa)*
journal, January 2012
- Molnár, István; Lopez, David; Wisecaver, Jennifer H.
- BMC Genomics, Vol. 13, Issue 1
Hydrocracking of the oils ofBotryococcus braunii to transport fuels
journal, January 1982
- Hillen, L. W.; Pollard, G.; Wake, L. V.
- Biotechnology and Bioengineering, Vol. 24, Issue 1
Production of C35 isoprenoids depends on H2 availability during cultivation of the hyperthermophile Methanococcus jannaschii
journal, October 2003
- Manquin, Brendan P.; Morgan, John A.; Ju, Jaeyeong
- Extremophiles, Vol. 8, Issue 1
Recombinant Squalene Synthase. A Mechanism for the Rearrangement of Presqualene Diphosphate to Squalene
journal, July 2002
- Blagg, Brian S. J.; Jarstfer, Michael B.; Rogers, Daniel H.
- Journal of the American Chemical Society, Vol. 124, Issue 30
Triterpene hydrocarbon production engineered into a metabolically versatile host- Rhodobacter capsulatus : Triterpene Hydrocarbon Production Engineered
journal, May 2015
- Khan, Nymul E.; Nybo, S. Eric; Chappell, Joe
- Biotechnology and Bioengineering, Vol. 112, Issue 8
Metabolic Diversification--Independent Assembly of Operon-Like Gene Clusters in Different Plants
journal, April 2008
- Field, B.; Osbourn, A. E.
- Science, Vol. 320, Issue 5875
Identification of unique mechanisms for triterpene biosynthesis in Botryococcus braunii
journal, July 2011
- Niehaus, T. D.; Okada, S.; Devarenne, T. P.
- Proceedings of the National Academy of Sciences, Vol. 108, Issue 30
Substrate specificity of squalene synthetase
journal, February 1980
- Tanetoshi, Koyama; Kyozo, Ogura; Shuichi, Seto
- Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, Vol. 617, Issue 2
Botryococcus—A planktonic green alga, the source of petroleum through the ages: Transmission electron microscopical studies of oil shales and petroleum source rocks
journal, January 1989
- Glikson, Miryam; Lindsay, Kay; Saxby, John
- Organic Geochemistry, Vol. 14, Issue 6
Botryococcus—A planktonic green alga, the source of petroleum through the ages: Transmission electron microscopical studies of oil shales and petroleum source rocks
journal, January 1989
- Glikson, Miryam; Lindsay, Kay; Saxby, John
- Organic Geochemistry, Vol. 14, Issue 6
Substrate selectivity of squalene synthetase
journal, June 1977
- Ortiz de Montellano, Paul R.; Wei, Jeng Shu; Vinson, Wayne A.
- Biochemistry, Vol. 16, Issue 12
Function-Structure Studies and Identification of Three Enzyme Domains Involved in the Catalytic Activity in Rat Hepatic Squalene Synthase
journal, May 1998
- Gu, Peide; Ishii, Yoshinori; Spencer, Thomas A.
- Journal of Biological Chemistry, Vol. 273, Issue 20
The discovery of a mevalonate-independent pathway for isoprenoid biosynthesis in bacteria, algae and higher plants†
journal, January 1999
- Rohmer, Michel; Rohmer, Michel
- Natural Product Reports, Vol. 16, Issue 5
The Influence of the Mineral Composition of the Medium on the Growth of Planktonic Algae: Part I. Methods and Culture Media
journal, August 1942
- Chu, S. P.
- The Journal of Ecology, Vol. 30, Issue 2
Substrate selectivity of squalene synthetase
journal, June 1977
- Ortiz de Montellano, Paul R.; Wei, Jeng Shu; Vinson, Wayne A.
- Biochemistry, Vol. 16, Issue 12
Biosynthesis of novel carotenoid families based on unnatural carbon backbones: A model for diversification of natural product pathways
journal, February 2006
- Tobias, Alexander V.; Arnold, Frances H.
- Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Vol. 1761, Issue 2
Structural insights into the catalytic mechanism of human squalene synthase
journal, January 2014
- Liu, Chia-I; Jeng, Wen-Yih; Chang, Wei-Jung
- Acta Crystallographica Section D Biological Crystallography, Vol. 70, Issue 2
Membrane homeoviscous adaptation in the piezo-hyperthermophilic archaeon Thermococcus barophilus
journal, October 2015
- Cario, Anaïs; Grossi, Vincent; Schaeffer, Philippe
- Frontiers in Microbiology, Vol. 6
Molecular Characterization of Squalene Synthase from the Green Microalga Botryococcus braunii, Race B
journal, January 2000
- Okada, Shigeru; Devarenne, Timothy P.; Chappell, Joseph
- Archives of Biochemistry and Biophysics, Vol. 373, Issue 2
Hydrocracking of the oils ofBotryococcus braunii to transport fuels
journal, January 1982
- Hillen, L. W.; Pollard, G.; Wake, L. V.
- Biotechnology and Bioengineering, Vol. 24, Issue 1
Constraints to commercialization of algal fuels
journal, September 2013
- Chisti, Yusuf
- Journal of Biotechnology, Vol. 167, Issue 3
Colony Organization in the Green Alga Botryococcus braunii (Race B) Is Specified by a Complex Extracellular Matrix
journal, August 2012
- Weiss, Taylor L.; Roth, Robyn; Goodson, Carrie
- Eukaryotic Cell, Vol. 11, Issue 12
Building terpene production platforms in yeast: Building Terpene Production Platforms in Yeast
journal, May 2015
- Zhuang, Xun; Chappell, Joe
- Biotechnology and Bioengineering, Vol. 112, Issue 9
Recombinant Squalene Synthase. A Mechanism for the Rearrangement of Presqualene Diphosphate to Squalene
journal, July 2002
- Blagg, Brian S. J.; Jarstfer, Michael B.; Rogers, Daniel H.
- Journal of the American Chemical Society, Vol. 124, Issue 30
Biosynthesis of Squalene from Farnesyl Diphosphate in Bacteria: Three Steps Catalyzed by Three Enzymes
journal, April 2015
- Pan, Jian-Jung; Solbiati, Jose O.; Ramamoorthy, Gurusankar
- ACS Central Science, Vol. 1, Issue 2
Botryococcus braunii: a rich source for hydrocarbons and related ether lipids
journal, December 2004
- Metzger, P.; Largeau, C.
- Applied Microbiology and Biotechnology, Vol. 66, Issue 5
Global evaluation of biofuel potential from microalgae
journal, May 2014
- Moody, J. W.; McGinty, C. M.; Quinn, J. C.
- Proceedings of the National Academy of Sciences, Vol. 111, Issue 23
Binding Modes of Zaragozic Acid A to Human Squalene Synthase and Staphylococcal Dehydrosqualene Synthase
journal, April 2012
- Liu, Chia-I; Jeng, Wen-Yih; Chang, Wei-Jung
- Journal of Biological Chemistry, Vol. 287, Issue 22
Structure and Chemistry of a new Chemical race of Botryococcus Braunii (Chlorophyceae) that Produces Lycopadiene, a Tetraterpenoid Hydrocarbon1
journal, June 1990
- Metzger, Pierre; Allard, Beatrice; Casadevall, Eliette
- Journal of Phycology, Vol. 26, Issue 2
A C35 Carotenoid Biosynthetic Pathway
journal, June 2003
- Umeno, D.; Arnold, F. H.
- Applied and Environmental Microbiology, Vol. 69, Issue 6
Identification of unique mechanisms for triterpene biosynthesis in Botryococcus braunii
journal, July 2011
- Niehaus, T. D.; Okada, S.; Devarenne, T. P.
- Proceedings of the National Academy of Sciences, Vol. 108, Issue 30
Constraints to commercialization of algal fuels
journal, September 2013
- Chisti, Yusuf
- Journal of Biotechnology, Vol. 167, Issue 3
Metabolic engineering for the production of hydrocarbon fuels
journal, June 2015
- Lee, Sang Yup; Kim, Hye Mi; Cheon, Seungwoo
- Current Opinion in Biotechnology, Vol. 33
Metabolic Diversification--Independent Assembly of Operon-Like Gene Clusters in Different Plants
journal, April 2008
- Field, B.; Osbourn, A. E.
- Science, Vol. 320, Issue 5875
The discovery of a mevalonate-independent pathway for isoprenoid biosynthesis in bacteria, algae and higher plants†
journal, January 1999
- Rohmer, Michel; Rohmer, Michel
- Natural Product Reports, Vol. 16, Issue 5
Genetic and biochemical analyses of the biosynthesis of the yellow carotenoid 4,4'-diaponeurosporene of Staphylococcus aureus.
journal, December 1994
- Wieland, B.; Feil, C.; Gloria-Maercker, E.
- Journal of Bacteriology, Vol. 176, Issue 24
Isolation and identification of “bacteriorhodopsin” and minor C40-carotenoids in Halobacterium cutirubrum
journal, August 1973
- Kushwaha, S. C.; Kates, M.
- Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, Vol. 316, Issue 2
Bio-crude transcriptomics: Gene discovery and metabolic network reconstruction for the biosynthesis of the terpenome of the hydrocarbon oil-producing green alga, Botryococcus braunii race B (Showa)*
journal, January 2012
- Molnár, István; Lopez, David; Wisecaver, Jennifer H.
- BMC Genomics, Vol. 13, Issue 1
Function-structure studies and identification of three enzyme domains involved in the catalytic activity in rat hepatic squalene synthase
journal, July 1998
- Gu, Peide; Ishii, Yoshinori; Spencer, Thomas A.
- Journal of Biological Chemistry, Vol. 273, Issue 27
C40 monoaromatic lycopane derivatives as indicators of the contribution of the alga Botryococcus braunii race L to the organic matter of Messel oil shale (Eocene, Germany)
journal, May 2006
- Adam, Pierre; Schaeffer, Philippe; Albrecht, Pierre
- Organic Geochemistry, Vol. 37, Issue 5
Membrane homeoviscous adaptation in the piezo-hyperthermophilic archaeon Thermococcus barophilus
journal, October 2015
- Cario, Anaïs; Grossi, Vincent; Schaeffer, Philippe
- Frontiers in Microbiology, Vol. 6
Biosynthesis of Non-Head-to-Tail Isoprenoids. Synthesis of 1'-1 and 1'-3 Structures by Recombinant Yeast Squalene Synthase
journal, February 1995
- Zhang, Dong-lu; Poulter, C. Dale
- Journal of the American Chemical Society, Vol. 117, Issue 5
Stereochemistry of trans-phytoene synthesis. Lycopersene as a carotene precursor and a mechanism for the synthesis of cis- and trans-phytoene
journal, March 1974
- Gregonis, Donald E.; Rilling, Hans C.
- Biochemistry, Vol. 13, Issue 7
Biosynthesis of novel carotenoid families based on unnatural carbon backbones: A model for diversification of natural product pathways
journal, February 2006
- Tobias, Alexander V.; Arnold, Frances H.
- Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Vol. 1761, Issue 2
Energy innovation potential of oleaginous microalgae
journal, November 2012
- Yoshida, Masaki; Tanabe, Yuuhiko; Yonezawa, Natsuki
- Biofuels, Vol. 3, Issue 6
Functional Identification of Triterpene Methyltransferases from Botryococcus braunii Race B
journal, January 2012
- Niehaus, Tom D.; Kinison, Scott; Okada, Shigeru
- Journal of Biological Chemistry, Vol. 287, Issue 11
Botryococcus braunii : A Renewable Source of Hydrocarbons and Other Chemicals
journal, January 2002
- Banerjee, Anirban; Sharma, Rohit; Chisti, Yusuf
- Critical Reviews in Biotechnology, Vol. 22, Issue 3
Subcellular localization of squalene synthase in rat hepatic cells. Biochemical and immunochemical evidence
journal, June 1993
- Stamellos, K. D.; Shackelford, J. E.; Shechter, I.
- Journal of Biological Chemistry, Vol. 268, Issue 17
Biosynthesis of Prelycopersene Pyrophosphate and Lycopersene by Squalene Synthetase
journal, April 1973
- Qureshi, Asaf A.; Barnes, Francis J.; Semmler, Erich J.
- Journal of Biological Chemistry, Vol. 248, Issue 8
Prelycopersene Pyrophosphate and Lycopersene
journal, April 1973
- Barnes, Francis J.; Qureshi, Asaf A.; Semmler, Erich J.
- Journal of Biological Chemistry, Vol. 248, Issue 8
Squalestatin 1, a potent inhibitor of squalene synthase, which lowers serum cholesterol in vivo.
journal, June 1992
- Baxter, A.; Fitzgerald, B. J.; Hutson, J. L.
- Journal of Biological Chemistry, Vol. 267, Issue 17
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journal, July 2018
- Zhang, Xiaolin; Wen, Fang; Xu, Zhenyu
- Journal of Applied Phycology, Vol. 31, Issue 1
Comparative transcriptome analyses of oleaginous Botryococcus braunii race A reveal significant differences in gene expression upon cobalt enrichment
journal, December 2018
- Cheng, Pengfei; Zhou, Chengxu; Wang, Yan
- Biotechnology for Biofuels, Vol. 11, Issue 1