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Title: Acetylation of cell wall is required for structural integrity of the leaf surface and exerts a global impact on plant stress responses

Here we report that the epidermis on leaves protects plants from pathogen invasion and provides a waterproof barrier. It consists of a layer of cells that is surrounded by thick cell walls, which are partially impregnated by highly hydrophobic cuticular components. We show that the Arabidopsis T-DNA insertion mutants of REDUCED WALL ACETYLATION 2 (rwa2), previously identified as having reduced O-acetylation of both pectins and hemicelluloses, exhibit pleiotrophic phenotype on the leaf surface. The cuticle layer appeared diffused and was significantly thicker and underneath cell wall layer was interspersed with electron-dense deposits. A large number of trichomes were collapsed and surface permeability of the leaves was enhanced in rwa2 as compared to the wild type. A massive reprogramming of the transcriptome was observed in rwa2 as compared to the wild type, including a coordinated up-regulation of genes involved in responses to abiotic stress, particularly detoxification of reactive oxygen species and defense against microbial pathogens (e.g., lipid transfer proteins, peroxidases). In accordance, peroxidase activities were found to be elevated in rwa2 as compared to the wild type. These results indicate that cell wall acetylation is essential for maintaining the structural integrity of leaf epidermis, and that reduction of cell wall acetylationmore » leads to global stress responses in Arabidopsis.« less
Authors:
 [1] ;  [1] ;  [1] ;  [2] ;  [3] ;  [3] ;  [1] ;  [4] ;  [5] ;  [6] ;  [1]
  1. Copenhagen Plant Science Center, Frederiksberg (Denmark); Univ. of Copenhagen (Denmark)
  2. Univ. of California, Davis, CA (United States)
  3. Univ. of Copenhagen (Denmark)
  4. Univ. of Lausanne, Lausanne (Switzerland)
  5. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Univ. of California, Berkeley, CA (United States)
  6. Univ. of California, Davis, CA (United States); Danish National Research Foundation Center DynaMO, Frederiksberg (Denmark)
Publication Date:
OSTI Identifier:
1254007
Grant/Contract Number:
AC02-05CH11231; 1339125; 1021861; 001-2011-4; 12-131834
Type:
Accepted Manuscript
Journal Name:
Frontiers in Plant Science
Additional Journal Information:
Journal Volume: 6; Journal ID: ISSN 1664-462X
Publisher:
Frontiers Research Foundation
Research Org:
Univ. of California, Berkeley, CA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES cellwallacetylation; trichomes; cuticles; epidermis; Botrytiscinerea; peroxidase; mRNAsequencing