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Title: Cellulolytic potential under environmental changes in microbial communities from grassland litter

In many ecosystems, global changes are likely to profoundly affect microorganisms. In Southern California, changes in precipitation and nitrogen deposition may influence the composition and functional potential of microbial communities and their resulting ability to degrade plant material. To test whether such environmental changes impact the distribution of functional groups involved in leaf litter degradation, we determined how the genomic diversity of microbial communities in a semi-arid grassland ecosystem changed under reduced precipitation or increased N deposition. We monitored communities seasonally over a period of 2 years to place environmental change responses into the context of natural variation. Fungal and bacterial communities displayed strong seasonal patterns, Fungi being mostly detected during the dry season whereas Bacteria were common during wet periods. Most putative cellulose degraders were associated with 33 bacterial genera and predicted to constitute 18% of the microbial community. Precipitation reduction reduced bacterial abundance and cellulolytic potential whereas nitrogen addition did not affect the cellulolytic potential of the microbial community. Finally, we detected a strong correlation between the frequencies of genera of putative cellulose degraders and cellulase genes. Thus, microbial taxonomic composition was predictive of cellulolytic potential. This work provides a framework for how environmental changes affect microorganisms responsiblemore » for plant litter deconstruction.« less
Authors:
 [1] ;  [2] ;  [3] ;  [3] ;  [4] ;  [3] ;  [2]
  1. Univ. of California, Irvine, CA (United States). Dept. of Earth System Science; California State Univ. (CalState), Long Beach, CA (United States)
  2. Univ. of California, Irvine, CA (United States). Dept. of Earth System Science; Univ. of California, Irvine, CA (United States). Dept. of Ecology and Evolutionary Biology
  3. Univ. of California, Irvine, CA (United States). Dept. of Ecology and Evolutionary Biology
  4. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Earth Sciences Division; Univ. of California, Berkeley, CA (United States). Dept. of Environmental Science
Publication Date:
OSTI Identifier:
1254001
Grant/Contract Number:
PS02-09ER09-25; OCE-1046297
Type:
Accepted Manuscript
Journal Name:
Frontiers in Microbiology
Additional Journal Information:
Journal Volume: 5; Journal ID: ISSN 1664-302X
Publisher:
Frontiers Research Foundation
Research Org:
University of California, Berkeley, CA (United States)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES cellulase; metagenomics; leaf litter; global change; microbial community composition