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Title: X-ray laser diffraction for structure determination of the rhodopsin-arrestin complex

Here, serial femtosecond X-ray crystallography (SFX) using an X-ray free electron laser (XFEL) is a recent advancement in structural biology for solving crystal structures of challenging membrane proteins, including G-protein coupled receptors (GPCRs), which often only produce microcrystals. An XFEL delivers highly intense X-ray pulses of femtosecond duration short enough to enable the collection of single diffraction images before significant radiation damage to crystals sets in. Here we report the deposition of the XFEL data and provide further details on crystallization, XFEL data collection and analysis, structure determination, and the validation of the structural model. The rhodopsin-arrestin crystal structure solved with SFX represents the first near-atomic resolution structure of a GPCR-arrestin complex, provides structural insights into understanding of arrestin-mediated GPCR signaling, and demonstrates the great potential of this SFX-XFEL technology for accelerating crystal structure determination of challenging proteins and protein complexes.
Authors:
 [1] ;  [1] ;  [2] ;  [1] ;  [1] ;  [3] ;  [4] ;  [2] ;  [2] ;  [4] ;  [5] ;  [1] ;  [1] ;  [5] ;  [5] ;  [6] ;  [7] ;  [7] ;  [2] ;  [8] more »;  [2] ;  [9] ;  [10] ;  [4] ;  [1] ;  [11] « less
  1. Van Andel Research Institute, Grand Rapids, MI (United States)
  2. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany)
  3. Arizona State Univ., Tempe, AZ (United States)
  4. Univ. of Southern California, Los Angeles, CA (United States)
  5. SLAC National Accelerator Lab., Menlo Park, CA (United States)
  6. Swiss Light Source at Paul Scherrer Institute, Villigen (Switzerland)
  7. Trinity College, Dublin (Ireland)
  8. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); Arizona State Univ., Tempe, AZ (United States)
  9. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); Arizona State Univ., Tempe, AZ (United States)
  10. Univ. of Southern California, Los Angeles, CA (United States); Shanghai Tech Univ., Shanghai (China)
  11. Van Andel Research Institute, Grand Rapids, MI (United States); Chinese Academy of Sciences, Shanghai (China)
Publication Date:
OSTI Identifier:
1251413
Grant/Contract Number:
AC02-76SF00515
Type:
Accepted Manuscript
Journal Name:
Scientific Data
Additional Journal Information:
Journal Volume: 3; Journal ID: ISSN 2052-4463
Publisher:
Nature Publishing Group
Research Org:
SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)
Sponsoring Org:
USDOE Office of Science (SC)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES biological physics; G protein-coupled receptors; nanocrystallography