Multimodality hard-x-ray imaging of a chromosome with nanoscale spatial resolution
Abstract
Here, we developed a scanning hard x-ray microscope using a new class of x-ray nano-focusing optic called a multilayer Laue lens and imaged a chromosome with nanoscale spatial resolution. The combination of the hard x-ray's superior penetration power, high sensitivity to elemental composition, high spatial-resolution and quantitative analysis creates a unique tool with capabilities that other microscopy techniques cannot provide. Using this microscope, we simultaneously obtained absorption-, phase-, and fluorescence-contrast images of Pt-stained human chromosome samples. The high spatial-resolution of the microscope and its multi-modality imaging capabilities enabled us to observe the internal ultra-structures of a thick chromosome without sectioning it.
- Authors:
-
- Brookhaven National Lab. (BNL), Upton, NY (United States)
- Diamond Light Source Ltd., Oxfordshire (United Kingdom)
- Univ. College London, London (United Kingdom); Rutherford Appleton Lab., Oxfordshire (United Kingdom)
- Brookhaven National Lab. (BNL), Upton, NY (United States); Argonne National Lab. (ANL), Argonne, IL (United States)
- Publication Date:
- Research Org.:
- Argonne National Laboratory (ANL), Argonne, IL (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1248377
- Grant/Contract Number:
- AC02-06CH11357
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Scientific Reports
- Additional Journal Information:
- Journal Volume: 6; Journal ID: ISSN 2045-2322
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 77 NANOSCIENCE AND NANOTECHNOLOGY; 59 BASIC BIOLOGICAL SCIENCES; fluorescence imaging; imaging techniques
Citation Formats
Yan, Hanfei, Nazaretski, Evgeny, Lauer, Kenneth R., Huang, Xiaojing, Wagner, Ulrich, Rau, Christoph, Yusuf, Mohammed, Robinson, Ian K., Kalbfleisch, Sebastian, Li, Li, Bouet, Nathalie, Zhou, Juan, Conley, Ray, and Chu, Yong S. Multimodality hard-x-ray imaging of a chromosome with nanoscale spatial resolution. United States: N. p., 2016.
Web. doi:10.1038/srep20112.
Yan, Hanfei, Nazaretski, Evgeny, Lauer, Kenneth R., Huang, Xiaojing, Wagner, Ulrich, Rau, Christoph, Yusuf, Mohammed, Robinson, Ian K., Kalbfleisch, Sebastian, Li, Li, Bouet, Nathalie, Zhou, Juan, Conley, Ray, & Chu, Yong S. Multimodality hard-x-ray imaging of a chromosome with nanoscale spatial resolution. United States. https://doi.org/10.1038/srep20112
Yan, Hanfei, Nazaretski, Evgeny, Lauer, Kenneth R., Huang, Xiaojing, Wagner, Ulrich, Rau, Christoph, Yusuf, Mohammed, Robinson, Ian K., Kalbfleisch, Sebastian, Li, Li, Bouet, Nathalie, Zhou, Juan, Conley, Ray, and Chu, Yong S. Fri .
"Multimodality hard-x-ray imaging of a chromosome with nanoscale spatial resolution". United States. https://doi.org/10.1038/srep20112. https://www.osti.gov/servlets/purl/1248377.
@article{osti_1248377,
title = {Multimodality hard-x-ray imaging of a chromosome with nanoscale spatial resolution},
author = {Yan, Hanfei and Nazaretski, Evgeny and Lauer, Kenneth R. and Huang, Xiaojing and Wagner, Ulrich and Rau, Christoph and Yusuf, Mohammed and Robinson, Ian K. and Kalbfleisch, Sebastian and Li, Li and Bouet, Nathalie and Zhou, Juan and Conley, Ray and Chu, Yong S.},
abstractNote = {Here, we developed a scanning hard x-ray microscope using a new class of x-ray nano-focusing optic called a multilayer Laue lens and imaged a chromosome with nanoscale spatial resolution. The combination of the hard x-ray's superior penetration power, high sensitivity to elemental composition, high spatial-resolution and quantitative analysis creates a unique tool with capabilities that other microscopy techniques cannot provide. Using this microscope, we simultaneously obtained absorption-, phase-, and fluorescence-contrast images of Pt-stained human chromosome samples. The high spatial-resolution of the microscope and its multi-modality imaging capabilities enabled us to observe the internal ultra-structures of a thick chromosome without sectioning it.},
doi = {10.1038/srep20112},
journal = {Scientific Reports},
number = ,
volume = 6,
place = {United States},
year = {Fri Feb 05 00:00:00 EST 2016},
month = {Fri Feb 05 00:00:00 EST 2016}
}
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