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Title: Advancing understanding of microbial bioenergy conversion processes by activity-based protein profiling

Here, the development of renewable biofuels is a global priority, but success will require novel technologies that greatly improve our understanding of microbial systems biology. An approach with great promise in enabling functional characterization of microbes is activity-based protein profiling (ABPP), which employs chemical probes to directly measure enzyme function in discrete enzyme classes in vivo and/or in vitro, thereby facilitating the rapid discovery of new biocatalysts and enabling much improved biofuel production platforms. We review general design strategies in ABPP, and highlight recent advances that are or could be pivotal to biofuels processes including applications of ABPP to cellulosic bioethanol, biodiesel, and phototrophic production of hydrocarbons. We also examine the key challenges and opportunities of ABPP in renewable biofuels research. The integration of ABPP with molecular and systems biology approaches will shed new insight on the catalytic and regulatory mechanisms of functional enzymes and their synergistic effects in the field of biofuels production.
Authors:
 [1] ;  [2] ;  [2] ;  [2] ;  [2] ;  [2]
  1. Beijing Univ. of Chemical Technology, Beijing (China)
  2. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Publication Date:
OSTI Identifier:
1243254
Report Number(s):
PNNL-SA--107650
Journal ID: ISSN 1754-6834; 48680; 48672; KP1601010
Grant/Contract Number:
AC05-76RL01830
Type:
Accepted Manuscript
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Volume: 8; Journal ID: ISSN 1754-6834
Publisher:
BioMed Central
Research Org:
Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
activity-based protein profiling (ABPP); cellulosic bioethanol; biodiesel; protein redox; proteomics; environmental molecular sciences laboratory