Atom probe tomographic mapping directly reveals the atomic distribution of phosphorus in resin embedded ferritin
Abstract
In this study, we report the atomic-scale analysis of biological interfaces using atom probe tomography. Embedding the protein ferritin in an organic polymer resin lacking nitrogen provided chemical contrast to visualize atomic distributions and distinguish organic-organic and organic-inorganic interfaces. The sample preparation method can be directly extended to further enhance the study of biological, organic and inorganic nanomaterials relevant to health, energy or the environment.
- Authors:
-
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Qatar Environment and Energy Research Institute, Doha (Qatar)
- Publication Date:
- Research Org.:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1243203
- Report Number(s):
- PNNL-SA-112512
Journal ID: ISSN 2045-2322; 48671
- Grant/Contract Number:
- AC05-76RL01830
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Scientific Reports
- Additional Journal Information:
- Journal Volume: 6; Journal ID: ISSN 2045-2322
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 36 MATERIALS SCIENCE; Environmental Molecular Sciences Laboratory; biomaterials - proteins; mass spectrometry; membrane structure and assembly; microscopy
Citation Formats
Perea, Daniel E., Liu, Jia, Bartrand, Jonah A. G., Dicken, Quinten G., Thevuthasan, Suntharampillai Theva, Browning, Nigel D., and Evans, James E. Atom probe tomographic mapping directly reveals the atomic distribution of phosphorus in resin embedded ferritin. United States: N. p., 2016.
Web. doi:10.1038/srep22321.
Perea, Daniel E., Liu, Jia, Bartrand, Jonah A. G., Dicken, Quinten G., Thevuthasan, Suntharampillai Theva, Browning, Nigel D., & Evans, James E. Atom probe tomographic mapping directly reveals the atomic distribution of phosphorus in resin embedded ferritin. United States. https://doi.org/10.1038/srep22321
Perea, Daniel E., Liu, Jia, Bartrand, Jonah A. G., Dicken, Quinten G., Thevuthasan, Suntharampillai Theva, Browning, Nigel D., and Evans, James E. Mon .
"Atom probe tomographic mapping directly reveals the atomic distribution of phosphorus in resin embedded ferritin". United States. https://doi.org/10.1038/srep22321. https://www.osti.gov/servlets/purl/1243203.
@article{osti_1243203,
title = {Atom probe tomographic mapping directly reveals the atomic distribution of phosphorus in resin embedded ferritin},
author = {Perea, Daniel E. and Liu, Jia and Bartrand, Jonah A. G. and Dicken, Quinten G. and Thevuthasan, Suntharampillai Theva and Browning, Nigel D. and Evans, James E.},
abstractNote = {In this study, we report the atomic-scale analysis of biological interfaces using atom probe tomography. Embedding the protein ferritin in an organic polymer resin lacking nitrogen provided chemical contrast to visualize atomic distributions and distinguish organic-organic and organic-inorganic interfaces. The sample preparation method can be directly extended to further enhance the study of biological, organic and inorganic nanomaterials relevant to health, energy or the environment.},
doi = {10.1038/srep22321},
journal = {Scientific Reports},
number = ,
volume = 6,
place = {United States},
year = {Mon Feb 29 00:00:00 EST 2016},
month = {Mon Feb 29 00:00:00 EST 2016}
}
Free Publicly Available Full Text
Publisher's Version of Record
Other availability
Search WorldCat to find libraries that may hold this journal
Cited by: 50 works
Citation information provided by
Web of Science
Web of Science
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Multimodal nanoparticles as alignment and correlation markers in fluorescence/soft X-ray cryo-microscopy/tomography of nucleoplasmic reticulum and apoptosis in mammalian cells
journal, November 2014
- Hagen, Christoph; Werner, Stephan; Carregal-Romero, Susana
- Ultramicroscopy, Vol. 146
Imaging and elemental mapping of biological specimens with a dual-EDS dedicated scanning transmission electron microscope
journal, May 2013
- Wu, J. S.; Kim, A. M.; Bleher, R.
- Ultramicroscopy, Vol. 128
Compositional Mapping of the Surface and Interior of Mammalian Cells at Submicrometer Resolution
journal, February 2011
- Szakal, Christopher; Narayan, Kedar; Fu, Jing
- Analytical Chemistry, Vol. 83, Issue 4
Atom probe tomography
journal, June 2009
- Miller, M. K.; Forbes, R. G.
- Materials Characterization, Vol. 60, Issue 6
Atom-Probe Tomography
book, January 2014
- Miller, Michael K.; Forbes, Richard G.
- Springer New York, NY
On the current role of atom probe tomography in materials characterization and materials science
journal, October 2013
- Marquis, Emmanuelle A.; Bachhav, Mukesh; Chen, Yimeng
- Current Opinion in Solid State and Materials Science, Vol. 17, Issue 5
Grain boundary segregation engineering in metallic alloys: A pathway to the design of interfaces
journal, August 2014
- Raabe, D.; Herbig, M.; Sandlöbes, S.
- Current Opinion in Solid State and Materials Science, Vol. 18, Issue 4
Atom Probe Tomography of Electronic Materials
journal, August 2007
- Kelly, Thomas F.; Larson, David J.; Thompson, Keith
- Annual Review of Materials Research, Vol. 37, Issue 1
Hadean age for a post-magma-ocean zircon confirmed by atom-probe tomography
journal, February 2014
- Valley, John W.; Cavosie, Aaron J.; Ushikubo, Takayuki
- Nature Geoscience, Vol. 7, Issue 3
Nanoscale chemical tomography of buried organic–inorganic interfaces in the chiton tooth
journal, January 2011
- Gordon, Lyle M.; Joester, Derk
- Nature, Vol. 469, Issue 7329
Atom Probe Tomography of Apatites and Bone-Type Mineralized Tissues
journal, November 2012
- Gordon, Lyle M.; Tran, Lawrence; Joester, Derk
- ACS Nano, Vol. 6, Issue 12
Amorphous intergranular phases control the properties of rodent tooth enamel
journal, February 2015
- Gordon, L. M.; Cohen, M. J.; MacRenaris, K. W.
- Science, Vol. 347, Issue 6223
Atom Probe Microscopy of Self-Assembled Monolayers: Preliminary Results
journal, April 2010
- Gault, Baptiste; Yang, Wenrong; Ratinac, Kyle R.
- Langmuir, Vol. 26, Issue 8
Atom probe tomography analysis of poly(3-alkylthiophene)s
journal, February 2010
- Prosa, T. J.; Keeney, S. Kostrna; Kelly, T. F.
- Journal of Microscopy, Vol. 237, Issue 2
Three-Dimensional Atom Probe Tomography of Oxide, Anion, and Alkanethiolate Coatings on Gold
journal, July 2010
- Zhang, Yi; Hillier, Andrew C.
- Analytical Chemistry, Vol. 82, Issue 14
Towards Atom Probe Tomography of Hybrid Organic-Inorganic Nanoparticles
journal, August 2013
- Gordon, L. M.; Cohen, M. J.; Joester, D.
- Microscopy and Microanalysis, Vol. 19, Issue S2
Focused ion beam fabrication of solidified ferritin into nanoscale volumes for compositional analysis using atom probe tomography: FIB FABRICATION OF SOLIDIFIED FERRITIN
journal, August 2012
- Greene, M. E.; Kelly, T. F.; Larson, D. J.
- Journal of Microscopy, Vol. 247, Issue 3
Point-projection imaging of macromolecular contours
journal, January 1982
- Panitz, J. A.
- Journal of Microscopy, Vol. 125, Issue 1
Point-projection imaging of unstained ferritin clusters
journal, January 1982
- Panitz, J. A.
- Ultramicroscopy, Vol. 7, Issue 3
Mineralization in Ferritin: An Efficient Means of Iron Storage
journal, June 1999
- Chasteen, N. Dennis; Harrison, Pauline M.
- Journal of Structural Biology, Vol. 126, Issue 3
In situ site-specific specimen preparation for atom probe tomography
journal, February 2007
- Thompson, K.; Lawrence, D.; Larson, D. J.
- Ultramicroscopy, Vol. 107, Issue 2-3
Incorporation and release of inorganic phosphate in horse spleen ferritin
journal, May 1978
- Trefry, A.; Harrison, P. M.
- Biochemical Journal, Vol. 171, Issue 2
Structure and composition of ferritin cores isolated from human spleen, limpet (Patella vulgata) hemolymph and bacterial (Pseudomonas aeruginosa) cells
journal, March 1986
- Mann, Stephen; Bannister, Joseph V.; Williams, Robert J. P.
- Journal of Molecular Biology, Vol. 188, Issue 2
The SLC34 family of sodium-dependent phosphate transporters
journal, December 2013
- Wagner, Carsten A.; Hernando, Nati; Forster, Ian C.
- Pflügers Archiv - European Journal of Physiology, Vol. 466, Issue 1
Sodium-dependent silicate transport in the apochlorotic marine diatom Nitzschia alba
journal, November 1980
- Bhattacharyya, P.; Volcani, B. E.
- Proceedings of the National Academy of Sciences, Vol. 77, Issue 11
Quantitative X-Ray Microanalysis of Ultra-Thin Resin-Embedded Biological Samples
book, January 1992
- Elder, Hugh Y.; Wilson, Stuart M.; Nicholson, W. A. Patrick
- Electron Microbeam Analysis. Mikrochimica Acta
In situ development of ion bombarded poly(methylmethacrylate) resist in a reactive gas ambient
journal, May 1988
- Gamo, Kenji
- Journal of Vacuum Science & Technology B: Microelectronics and Nanometer Structures, Vol. 6, Issue 3
Local Electrode Atom Probe Tomography: A User's Guide
book, January 2013
- Larson, David J.; Prosa, Ty J.; Ulfig, Robert M.
Data Processing and Reconstruction
book, January 2013
- Larson, David J.; Prosa, Ty J.; Ulfig, Robert M.
- Local Electrode Atom Probe Tomography
Imaging and elemental mapping of biological specimens with a dual-EDS dedicated scanning transmission electron microscope
journal, May 2013
- Wu, J. S.; Kim, A. M.; Bleher, R.
- Ultramicroscopy, Vol. 128
Three-Dimensional Atom Probe Tomography of Oxide, Anion, and Alkanethiolate Coatings on Gold
journal, July 2010
- Zhang, Yi; Hillier, Andrew C.
- Analytical Chemistry, Vol. 82, Issue 14
Compositional Mapping of the Surface and Interior of Mammalian Cells at Submicrometer Resolution
journal, February 2011
- Szakal, Christopher; Narayan, Kedar; Fu, Jing
- Analytical Chemistry, Vol. 83, Issue 4
Atom Probe Microscopy of Self-Assembled Monolayers: Preliminary Results
journal, April 2010
- Gault, Baptiste; Yang, Wenrong; Ratinac, Kyle R.
- Langmuir, Vol. 26, Issue 8
Atom Probe Tomography of Apatites and Bone-Type Mineralized Tissues
journal, November 2012
- Gordon, Lyle M.; Tran, Lawrence; Joester, Derk
- ACS Nano, Vol. 6, Issue 12
Nanoscale chemical tomography of buried organic–inorganic interfaces in the chiton tooth
journal, January 2011
- Gordon, Lyle M.; Joester, Derk
- Nature, Vol. 469, Issue 7329
Incorporation and release of inorganic phosphate in horse spleen ferritin
journal, May 1978
- Trefry, A.; Harrison, P. M.
- Biochemical Journal, Vol. 171, Issue 2
Sodium-dependent silicate transport in the apochlorotic marine diatom Nitzschia alba
journal, November 1980
- Bhattacharyya, P.; Volcani, B. E.
- Proceedings of the National Academy of Sciences, Vol. 77, Issue 11
Works referencing / citing this record:
Polymer on Top: Current Limits and Future Perspectives of Quantitatively Evaluating Surface Grafting
journal, March 2018
- Michalek, Lukas; Barner, Leonie; Barner-Kowollik, Christopher
- Advanced Materials, Vol. 30, Issue 21
Direct Imaging of Dopant and Impurity Distributions in 2D MoS 2
journal, January 2020
- Kim, Se‐Ho; Lim, Joohyun; Sahu, Rajib
- Advanced Materials, Vol. 32, Issue 8
Biomineralization at Titanium Revealed by Correlative 4D Tomographic and Spectroscopic Methods
journal, May 2018
- Wang, Xiaoyue; Langelier, Brian; Shah, Furqan A.
- Advanced Materials Interfaces, Vol. 5, Issue 14
Ionic Dependence of Gelatin Hydrogel Architecture Explored Using Small and Very Small Angle Neutron Scattering Technique
journal, May 2018
- Wu, Baohu; Siglreitmeier, Maria; Debus, Christian
- Macromolecular Bioscience, Vol. 18, Issue 6
Atom Probe Tomography for 3D Structural and Chemical Analysis of Individual Proteins
journal, May 2019
- Sundell, Gustav; Hulander, Mats; Pihl, Astrid
- Small, Vol. 15, Issue 24
A near atomic-scale view at the composition of amyloid-beta fibrils by atom probe tomography
journal, December 2018
- Rusitzka, Kristiane A. K.; Stephenson, Leigh T.; Szczepaniak, Agnieszka
- Scientific Reports, Vol. 8, Issue 1
Nanometer-Scale Chemistry of a Calcite Biomineralization Template: Implications for Skeletal Composition and Nucleation
journal, October 2016
- Branson, Oscar; Bonnin, Elisa A.; Perea, Daniel E.
- Proceedings of the National Academy of Sciences, Vol. 113, Issue 46
Visualizing the iron atom exchange front in the Fe(II)-catalyzed recrystallization of goethite by atom probe tomography
journal, February 2019
- Taylor, Sandra D.; Liu, Jia; Zhang, Xin
- Proceedings of the National Academy of Sciences, Vol. 116, Issue 8
An environmental transfer hub for multimodal atom probe tomography
journal, May 2017
- Perea, Daniel E.; Gerstl, Stephan S. A.; Chin, Jackson
- Advanced Structural and Chemical Imaging, Vol. 3, Issue 1
Preliminary Data on the Nanoscale Chemical Characterization of the Inter-Crystalline Organic Matrix of a Calcium Carbonate Biomineral
journal, May 2018
- Pérez-Huerta, Alberto; Laiginhas, Fernando
- Minerals, Vol. 8, Issue 6
An environmental transfer hub for multimodal atom probe tomography
text, January 2017
- Gerstl, Stephan S. A.; Perea, Daniel E.; Gerstl, Stephan S. A.
- ETH Zurich
A near atomic-scale view at the composition of amyloid-beta fibrils by atom probe tomography
journal, December 2018
- Rusitzka, Kristiane A. K.; Stephenson, Leigh T.; Szczepaniak, Agnieszka
- Scientific Reports, Vol. 8, Issue 1
An environmental transfer hub for multimodal atom probe tomography
journal, May 2017
- Perea, Daniel E.; Gerstl, Stephan S. A.; Chin, Jackson
- Advanced Structural and Chemical Imaging, Vol. 3, Issue 1