Use of De Novo transcriptome libraries to characterize a novel oleaginous marine Chlorella species during the accumulation of triacylglycerols
Abstract
Here, marine chlorophytes of the genus Chlorella are unicellular algae capable of accumulating a high proportion of cellular lipids that can be used for biodiesel production. In this study, we examined the broad physiological capabilities of a subtropical strain (C596) of Chlorella sp. “SAG-211-18” including its heterotrophic growth and tolerance to low salt.We found that the alga replicates more slowly at diluted salt concentrations and can grow on a wide range of carbon substrates in the dark.We then sequenced the RNA of Chlorella strain C596 to elucidate key metabolic genes and investigate the transcriptomic response of the organism when transitioning from a nutrient-replete to a nutrient-deficient condition when neutral lipids accumulate. Specific transcripts encoding for enzymes involved in both starch and lipid biosynthesis, among others, were up-regulated as the cultures transitioned into a lipid-accumulating state whereas photosynthesis-related genes were down-regulated. Transcripts encoding for two of the up-regulated enzymes—a galactoglycerolipid lipase and a diacylglyceride acyltransferase—were also monitored by reverse transcription quantitative polymerase chain reaction assays. The results of these assays confirmed the transcriptome-sequencing data. The present transcriptomic study will assist in the greater understanding, more effective application, and efficient design of Chlorella-based biofuel production systems.
- Authors:
-
- Cornell Univ., Ithaca, NY (United States)
- San Francisco State Univ., Tiburon, CA (United States)
- Peking Union Medical College, Beijing (China)
- Publication Date:
- Research Org.:
- Cornell Univ., Ithaca, NY (United States)
- Sponsoring Org.:
- USDOE Office of Energy Efficiency and Renewable Energy (EERE)
- OSTI Identifier:
- 1242369
- Grant/Contract Number:
- EE0003371
- Resource Type:
- Accepted Manuscript
- Journal Name:
- PLoS ONE
- Additional Journal Information:
- Journal Volume: 11; Journal Issue: 2; Journal ID: ISSN 1932-6203
- Publisher:
- Public Library of Science
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS; lipids; transcriptome analysis; lipases; ribosomal RNA; algae; sequence database; chlamydomonas reinhardtii; RNA extraction
Citation Formats
Mansfeldt, Cresten B., Richter, Lubna V., Ahner, Beth A., Cochlan, William P., Richardson, Ruth E., and Chen, Shilin. Use of De Novo transcriptome libraries to characterize a novel oleaginous marine Chlorella species during the accumulation of triacylglycerols. United States: N. p., 2016.
Web. doi:10.1371/journal.pone.0147527.
Mansfeldt, Cresten B., Richter, Lubna V., Ahner, Beth A., Cochlan, William P., Richardson, Ruth E., & Chen, Shilin. Use of De Novo transcriptome libraries to characterize a novel oleaginous marine Chlorella species during the accumulation of triacylglycerols. United States. https://doi.org/10.1371/journal.pone.0147527
Mansfeldt, Cresten B., Richter, Lubna V., Ahner, Beth A., Cochlan, William P., Richardson, Ruth E., and Chen, Shilin. Wed .
"Use of De Novo transcriptome libraries to characterize a novel oleaginous marine Chlorella species during the accumulation of triacylglycerols". United States. https://doi.org/10.1371/journal.pone.0147527. https://www.osti.gov/servlets/purl/1242369.
@article{osti_1242369,
title = {Use of De Novo transcriptome libraries to characterize a novel oleaginous marine Chlorella species during the accumulation of triacylglycerols},
author = {Mansfeldt, Cresten B. and Richter, Lubna V. and Ahner, Beth A. and Cochlan, William P. and Richardson, Ruth E. and Chen, Shilin},
abstractNote = {Here, marine chlorophytes of the genus Chlorella are unicellular algae capable of accumulating a high proportion of cellular lipids that can be used for biodiesel production. In this study, we examined the broad physiological capabilities of a subtropical strain (C596) of Chlorella sp. “SAG-211-18” including its heterotrophic growth and tolerance to low salt.We found that the alga replicates more slowly at diluted salt concentrations and can grow on a wide range of carbon substrates in the dark.We then sequenced the RNA of Chlorella strain C596 to elucidate key metabolic genes and investigate the transcriptomic response of the organism when transitioning from a nutrient-replete to a nutrient-deficient condition when neutral lipids accumulate. Specific transcripts encoding for enzymes involved in both starch and lipid biosynthesis, among others, were up-regulated as the cultures transitioned into a lipid-accumulating state whereas photosynthesis-related genes were down-regulated. Transcripts encoding for two of the up-regulated enzymes—a galactoglycerolipid lipase and a diacylglyceride acyltransferase—were also monitored by reverse transcription quantitative polymerase chain reaction assays. The results of these assays confirmed the transcriptome-sequencing data. The present transcriptomic study will assist in the greater understanding, more effective application, and efficient design of Chlorella-based biofuel production systems.},
doi = {10.1371/journal.pone.0147527},
journal = {PLoS ONE},
number = 2,
volume = 11,
place = {United States},
year = {Wed Feb 03 00:00:00 EST 2016},
month = {Wed Feb 03 00:00:00 EST 2016}
}
Free Publicly Available Full Text
Publisher's Version of Record
Other availability
Search WorldCat to find libraries that may hold this journal
Cited by: 11 works
Citation information provided by
Web of Science
Web of Science
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
A Broad Spectrum Artificial sea Water Medium for Coastal and open Ocean Phytoplankton
journal, March 1980
- Harrison, Paul J.; Waters, Rosemary E.; Taylor, F. J. R.
- Journal of Phycology, Vol. 16, Issue 1
Biosynthesis of Triacylglycerols (TAGs) in plants and algae
journal, April 2011
- Cagliari, Alexandro; Margis, Rogerio; Dos Santos Maraschin, Felipe
- International Journal of Plant Biology, Vol. 2, Issue 1
Evolution of an Artificial Seawater Medium: Improvements in Enriched Seawater, Artificial Water over the last two Decades
journal, December 2001
- Berges, John A.; Franklin, Daniel J.; Harrison, Paul J.
- Journal of Phycology, Vol. 37, Issue 6
The Chlorella variabilis NC64A Genome Reveals Adaptation to Photosymbiosis, Coevolution with Viruses, and Cryptic Sex
journal, September 2010
- Blanc, Guillaume; Duncan, Garry; Agarkova, Irina
- The Plant Cell, Vol. 22, Issue 9
High productivity cultivation of a heat-resistant microalga Chlorella sorokiniana for biofuel production
journal, March 2013
- Li, Tingting; Zheng, Yubin; Yu, Liang
- Bioresource Technology, Vol. 131
Biochemical Taxonomy and Molecular Phylogeny of the Genus Chlorella Sensu lato (Chlorophyta)
journal, June 1999
- Huss, Volker A. R.; Frank, Carola; Hartmann, Elke C.
- Journal of Phycology, Vol. 35, Issue 3
Carbon allocation under light and nitrogen resource gradients in two model marine phytoplankton 1
journal, March 2013
- Bittar, Thais B.; Lin, Yajuan; Sassano, Lara R.
- Journal of Phycology, Vol. 49, Issue 3
Production of biodiesel from microalgae oil (Chlorella protothecoides) by non-catalytic transesterification in supercritical methanol and ethanol: Process optimization
journal, February 2015
- Nan, Yue; Liu, Jiuxu; Lin, Ronghong
- The Journal of Supercritical Fluids, Vol. 97
Growth rate, organic carbon and nutrient removal rates of Chlorella sorokiniana in autotrophic, heterotrophic and mixotrophic conditions
journal, September 2013
- Kim, Sunjin; Park, Jeong-eun; Cho, Yong-Beom
- Bioresource Technology, Vol. 144
KEGG: Kyoto Encyclopedia of Genes and Genomes
journal, January 2000
- Kanehisa, Minoru; Goto, Susumu
- Nucleic Acids Research, Vol. 28, Issue 1, p. 27-30
Metabolic and cellular organization in evolutionarily diverse microalgae as related to biofuels production
journal, June 2013
- Hildebrand, Mark; Abbriano, Raffaela M.; Polle, Juergen EW
- Current Opinion in Chemical Biology, Vol. 17, Issue 3
Lipid Profile Remodeling in Response to Nitrogen Deprivation in the Microalgae Chlorella sp. (Trebouxiophyceae) and Nannochloropsis sp. (Eustigmatophyceae)
journal, August 2014
- Martin, Gregory J. O.; Hill, David R. A.; Olmstead, Ian L. D.
- PLoS ONE, Vol. 9, Issue 8
Biorefinery as a promising approach to promote microalgae industry: An innovative framework
journal, January 2015
- Zhu, Liandong
- Renewable and Sustainable Energy Reviews, Vol. 41
Induction of triacylglycerol production in Chlamydomonas reinhardtii: Comparative analysis of different element regimes
journal, March 2014
- Çakmak, Zeynep E.; Ölmez, Tolga T.; Çakmak, Turgay
- Bioresource Technology, Vol. 155
PHYTOPLANKTON LIPIDS: INTERSPECIFIC DIFFERENCES AND EFFECTS OF NITRATE, SILICATE AND LIGHT-DARK CYCLES 1
journal, December 1981
- Shifrin, Neil S.; Chisholm, Sallie W.
- Journal of Phycology, Vol. 17, Issue 4
Trimmomatic: a flexible trimmer for Illumina sequence data
journal, April 2014
- Bolger, Anthony M.; Lohse, Marc; Usadel, Bjoern
- Bioinformatics, Vol. 30, Issue 15
A Galactoglycerolipid Lipase Is Required for Triacylglycerol Accumulation and Survival Following Nitrogen Deprivation in Chlamydomonas reinhardtii
journal, November 2012
- Li, Xiaobo; Moellering, Eric R.; Liu, Bensheng
- The Plant Cell, Vol. 24, Issue 11
Perspectives on engineering strategies for improving biofuel production from microalgae — A critical review
journal, December 2014
- Ho, Shih-Hsin; Ye, Xiaoting; Hasunuma, Tomohisa
- Biotechnology Advances, Vol. 32, Issue 8
Fatty acid and lipid composition of 10 species of microalgae used in mariculture
journal, June 1989
- Volkman, J. K.; Jeffrey, S. W.; Nichols, P. D.
- Journal of Experimental Marine Biology and Ecology, Vol. 128, Issue 3
Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances
journal, May 2008
- Hu, Qiang; Sommerfeld, Milton; Jarvis, Eric
- The Plant Journal, Vol. 54, Issue 4
Lipid accumulation and biosynthesis genes response of the oleaginous Chlorella pyrenoidosa under three nutrition stressors
journal, January 2014
- Fan, Jianhua; Cui, Yanbin; Wan, Minxi
- Biotechnology for Biofuels, Vol. 7, Issue 1
Full-length transcriptome assembly from RNA-Seq data without a reference genome
journal, May 2011
- Grabherr, Manfred G.; Haas, Brian J.; Yassour, Moran
- Nature Biotechnology, Vol. 29, Issue 7
Draft genome sequence and genetic transformation of the oleaginous alga Nannochloropsis gaditana
journal, January 2012
- Radakovits, Randor; Jinkerson, Robert E.; Fuerstenberg, Susan I.
- Nature Communications, Vol. 3, Issue 1
Microalgae as biodiesel & biomass feedstocks: Review & analysis of the biochemistry, energetics & economics
journal, January 2010
- Williams, Peter J. le B.; Laurens, Lieve M. L.
- Energy & Environmental Science, Vol. 3, Issue 5
Studies of Marine Planktonic Diatoms: i. Cyclotella nana Hustedt, and Detonula Confervacea (Cleve) Gran.
journal, April 1962
- Guillard, Robert R. L.; Ryther, John H.
- Canadian Journal of Microbiology, Vol. 8, Issue 2
Effect of carbon source on growth and lipid accumulation in Chlorella sorokiniana GXNN01
journal, November 2009
- Qiao, Hongjin; Wang, Guangce
- Chinese Journal of Oceanology and Limnology, Vol. 27, Issue 4
Integrative genomics viewer
journal, January 2011
- Robinson, James T.; Thorvaldsdóttir, Helga; Winckler, Wendy
- Nature Biotechnology, Vol. 29, Issue 1
Screening and phylogenetic analysis of lipid-rich microalgae
journal, September 2015
- Li, Ling; Cui, Jing; Liu, Qian
- Algal Research, Vol. 11
Oil accumulation mechanisms of the oleaginous microalga Chlorella protothecoides revealed through its genome, transcriptomes, and proteomes
journal, January 2014
- Gao, Chunfang; Wang, Yun; Shen, Yue
- BMC Genomics, Vol. 15, Issue 1
Metabolism of acyl‐lipids in Chlamydomonas reinhardtii
journal, March 2015
- Li‐Beisson, Yonghua; Beisson, Fred; Riekhof, Wayne
- The Plant Journal, Vol. 82, Issue 3
De-novo assembly and characterization of Chlorella minutissima UTEX2341 transcriptome by paired-end sequencing and the identification of genes related to the biosynthesis of lipids for biodiesel
journal, February 2016
- Yu, Mingjia; Yang, Shanjun; Lin, Xiangzhi
- Marine Genomics, Vol. 25
Functional study of diacylglycerol acyltransferase type 2 family in Chlamydomonas reinhardtii
journal, June 2013
- Hung, Chun-Hsien; Ho, Ming-Yang; Kanehara, Kazue
- FEBS Letters, Vol. 587, Issue 15
SortMeRNA: fast and accurate filtering of ribosomal RNAs in metatranscriptomic data
journal, October 2012
- Kopylova, Evguenia; Noé, Laurent; Touzet, Hélène
- Bioinformatics, Vol. 28, Issue 24
A Broad Spectrum Artificial sea Water Medium for Coastal and open Ocean Phytoplankton1
journal, March 1980
- Harrison, Paul J.; Waters, Rosemary E.; Taylor, F. J. R.
- Journal of Phycology, Vol. 16, Issue 1
Targeting redox metabolism: the perfect storm induced by acrylamide poisoning in the brain
journal, January 2020
- Raldúa, Demetrio; Casado, Marta; Prats, Eva
- Scientific Reports, Vol. 10, Issue 1
Comparative Analysis of Eukaryotic Marine Microbial Assemblages from 18S rRNA Gene and Gene Transcript Clone Libraries by Using Different Methods of Extraction
journal, March 2012
- Koid, Amy; Nelson, William C.; Mraz, Amy
- Applied and Environmental Microbiology, Vol. 78, Issue 11
Phytoplankton Lipids: Interspecific Differences and Effects of Nitrate, Silicate and Light-Dark Cycles1
journal, December 1981
- Shifrin, Neil S.; Chisholm, Sallie W.
- Journal of Phycology, Vol. 17, Issue 4
Microalgae as biodiesel & biomass feedstocks: Review & analysis of the biochemistry, energetics & economics
journal, January 2010
- Williams, Peter J. le B.; Laurens, Lieve M. L.
- Energy & Environmental Science, Vol. 3, Issue 5
RNA-Seq Analysis of Sulfur-Deprived Chlamydomonas Cells Reveals Aspects of Acclimation Critical for Cell Survival
journal, June 2010
- González-Ballester, David; Casero, David; Cokus, Shawn
- The Plant Cell, Vol. 22, Issue 6
Carotenoid and lipid production by the autotrophic microalga Chlorella protothecoides under nutritional, salinity, and luminosity stress conditions
journal, November 2012
- Campenni’, L.; Nobre, B. P.; Santos, C. A.
- Applied Microbiology and Biotechnology, Vol. 97, Issue 3
Fatty acid and lipid composition of 10 species of microalgae used in mariculture
journal, June 1989
- Volkman, J. K.; Jeffrey, S. W.; Nichols, P. D.
- Journal of Experimental Marine Biology and Ecology, Vol. 128, Issue 3
TAG, You’re it! Chlamydomonas as a reference organism for understanding algal triacylglycerol accumulation
journal, June 2012
- Merchant, Sabeeha S.; Kropat, Janette; Liu, Bensheng
- Current Opinion in Biotechnology, Vol. 23, Issue 3
A Galactoglycerolipid Lipase Is Required for Triacylglycerol Accumulation and Survival Following Nitrogen Deprivation in Chlamydomonas reinhardtii
journal, November 2012
- Li, Xiaobo; Moellering, Eric R.; Liu, Bensheng
- The Plant Cell, Vol. 24, Issue 11
Perspectives on engineering strategies for improving biofuel production from microalgae — A critical review
journal, December 2014
- Ho, Shih-Hsin; Ye, Xiaoting; Hasunuma, Tomohisa
- Biotechnology Advances, Vol. 32, Issue 8
Genome, Functional Gene Annotation, and Nuclear Transformation of the Heterokont Oleaginous Alga Nannochloropsis oceanica CCMP1779
journal, November 2012
- Vieler, Astrid; Wu, Guangxi; Tsai, Chia-Hong
- PLoS Genetics, Vol. 8, Issue 11
Identifying reference genes with stable expression from high throughput sequence data
journal, January 2012
- Alexander, Harriet; Jenkins, Bethany D.; Rynearson, Tatiana A.
- Frontiers in Microbiology, Vol. 3
The effect of iron on growth, lipid accumulation, and gene expression profile of the freshwater microalga Chlorella sorokiniana
journal, September 2014
- Wan, Minxi; Jin, Xuejie; Xia, Jinlan
- Applied Microbiology and Biotechnology, Vol. 98, Issue 22
RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome
journal, August 2011
- Li, Bo; Dewey, Colin N.
- BMC Bioinformatics, Vol. 12, Issue 1
Biomass and lipid productivities of Chlorella vulgaris under autotrophic, heterotrophic and mixotrophic growth conditions
journal, March 2009
- Liang, Yanna; Sarkany, Nicolas; Cui, Yi
- Biotechnology Letters, Vol. 31, Issue 7
Gene expression profiles of the heterotrophic microalga Chlorella pyrenoidosa F-9
journal, January 2014
- Sun, X.; Xu, N. J.; Jiang, L. Z.
- Genetics and Molecular Research, Vol. 13, Issue 4
The Path to Triacylglyceride Obesity in the sta6 Strain of Chlamydomonas reinhardtii
journal, February 2014
- Goodenough, Ursula; Blaby, Ian; Casero, David
- Eukaryotic Cell, Vol. 13, Issue 5
Edible oils from microalgae: insights in TAG accumulation
journal, October 2014
- Klok, A. J.; Lamers, P. P.; Martens, D. E.
- Trends in Biotechnology, Vol. 32, Issue 10
Examination of Triacylglycerol Biosynthetic Pathways via De Novo Transcriptomic and Proteomic Analyses in an Unsequenced Microalga
journal, October 2011
- Guarnieri, Michael T.; Nag, Ambarish; Smolinski, Sharon L.
- PLoS ONE, Vol. 6, Issue 10
Elevated CO 2 improves lipid accumulation by increasing carbon metabolism in Chlorella sorokiniana
journal, May 2015
- Sun, Zhilan; Chen, Yi-Feng; Du, Jianchang
- Plant Biotechnology Journal, Vol. 14, Issue 2
Evolution of an Artificial Seawater Medium: Improvements in Enriched Seawater, Artificial Water over the last two Decades
journal, December 2001
- Berges, John A.; Franklin, Daniel J.; Harrison, Paul J.
- Journal of Phycology, Vol. 37, Issue 6
Biosynthesis of Triacylglycerols (TAGs) in plants and algae
journal, April 2011
- Cagliari, Alexandro; Margis, Rogerio; Dos Santos Maraschin, Felipe
- International Journal of Plant Biology, Vol. 2, Issue 1
Three Acyltransferases and Nitrogen-responsive Regulator Are Implicated in Nitrogen Starvation-induced Triacylglycerol Accumulation in Chlamydomonas
journal, March 2012
- Boyle, Nanette R.; Page, Mark Dudley; Liu, Bensheng
- Journal of Biological Chemistry, Vol. 287, Issue 19
Carbon allocation under light and nitrogen resource gradients in two model marine phytoplankton 1
journal, March 2013
- Bittar, Thais B.; Lin, Yajuan; Sassano, Lara R.
- Journal of Phycology, Vol. 49, Issue 3
Genome-wide CRISPR screen identifies PRC2 and KMT2D-COMPASS as regulators of distinct EMT trajectories that contribute differentially to metastasis
journal, April 2022
- Zhang, Yun; Donaher, Joana Liu; Das, Sunny
- Nature Cell Biology, Vol. 24, Issue 4
High productivity cultivation of a heat-resistant microalga Chlorella sorokiniana for biofuel production
journal, March 2013
- Li, Tingting; Zheng, Yubin; Yu, Liang
- Bioresource Technology, Vol. 131
Unified approach to Life Cycle Assessment between three unique algae biofuel facilities
journal, September 2015
- Bradley, Tom; Maga, Daniel; Antón, Sara
- Applied Energy, Vol. 154
Effect of carbon source on growth and lipid accumulation in Chlorella sorokiniana GXNN01
journal, November 2009
- Qiao, Hongjin; Wang, Guangce
- Chinese Journal of Oceanology and Limnology, Vol. 27, Issue 4
Growth rate, organic carbon and nutrient removal rates of Chlorella sorokiniana in autotrophic, heterotrophic and mixotrophic conditions
journal, September 2013
- Kim, Sunjin; Park, Jeong-eun; Cho, Yong-Beom
- Bioresource Technology, Vol. 144
Oil accumulation mechanisms of the oleaginous microalga Chlorella protothecoides revealed through its genome, transcriptomes, and proteomes
journal, January 2014
- Gao, Chunfang; Wang, Yun; Shen, Yue
- BMC Genomics, Vol. 15, Issue 1
Transcriptome sequencing of the marine microalga, Chlorella pyrenoidosa (Chlorophyta), and analysis of carbonic anhydrase expression under salt stress
journal, September 2014
- Sun, Xue; Wang, Weiwei; Shen, Jia
- Botanica Marina, Vol. 57, Issue 5
Examination of Triacylglycerol Biosynthetic Pathways via De Novo Transcriptomic and Proteomic Analyses in an Unsequenced Microalga
journal, October 2011
- Guarnieri, Michael T.; Nag, Ambarish; Smolinski, Sharon L.
- PLoS ONE, Vol. 6, Issue 10
Biochemical Taxonomy and Molecular Phylogeny of the Genus Chlorella Sensu lato (Chlorophyta)
journal, June 1999
- Huss, Volker A. R.; Frank, Carola; Hartmann, Elke C.
- Journal of Phycology, Vol. 35, Issue 3
Pathways of Lipid Metabolism in Marine Algae, Co-Expression Network, Bottlenecks and Candidate Genes for Enhanced Production of EPA and DHA in Species of Chromista
journal, November 2013
- Mühlroth, Alice; Li, Keshuai; Røkke, Gunvor
- Marine Drugs, Vol. 11, Issue 11
TAG, You’re it! Chlamydomonas as a reference organism for understanding algal triacylglycerol accumulation
journal, June 2012
- Merchant, Sabeeha S.; Kropat, Janette; Liu, Bensheng
- Current Opinion in Biotechnology, Vol. 23, Issue 3
Genome, Functional Gene Annotation, and Nuclear Transformation of the Heterokont Oleaginous Alga Nannochloropsis oceanica CCMP1779
journal, November 2012
- Vieler, Astrid; Wu, Guangxi; Tsai, Chia-Hong
- PLoS Genetics, Vol. 8, Issue 11
Culture of microalgae Chlorella minutissima for biodiesel feedstock production
journal, July 2011
- Tang, Haiying; Chen, Meng; Garcia, M. E. D.
- Biotechnology and Bioengineering, Vol. 108, Issue 10
Biorefinery as a promising approach to promote microalgae industry: An innovative framework
journal, January 2015
- Zhu, Liandong
- Renewable and Sustainable Energy Reviews, Vol. 41
Blast2GO: a universal tool for annotation, visualization and analysis in functional genomics research
journal, August 2005
- Conesa, A.; Gotz, S.; Garcia-Gomez, J. M.
- Bioinformatics, Vol. 21, Issue 18
Transcriptome Sequencing (RNA-seq) Analysis of the Effects of Metal Nanoparticle Exposure on the Transcriptome of Chlamydomonas reinhardtii
journal, May 2013
- Simon, D. F.; Domingos, R. F.; Hauser, C.
- Applied and Environmental Microbiology, Vol. 79, Issue 16
RNA-Seq Analysis of Sulfur-Deprived Chlamydomonas Cells Reveals Aspects of Acclimation Critical for Cell Survival
journal, June 2010
- González-Ballester, David; Casero, David; Cokus, Shawn
- The Plant Cell, Vol. 22, Issue 6
Production of biodiesel from microalgae oil (Chlorella protothecoides) by non-catalytic transesterification in supercritical methanol and ethanol: Process optimization
journal, February 2015
- Nan, Yue; Liu, Jiuxu; Lin, Ronghong
- The Journal of Supercritical Fluids, Vol. 97
Changes in Transcript Abundance in Chlamydomonas reinhardtii following Nitrogen Deprivation Predict Diversion of Metabolism
journal, October 2010
- Miller, Rachel; Wu, Guangxi; Deshpande, Rahul R.
- Plant Physiology, Vol. 154, Issue 4
Induction of triacylglycerol production in Chlamydomonas reinhardtii: Comparative analysis of different element regimes
journal, March 2014
- Çakmak, Zeynep E.; Ölmez, Tolga T.; Çakmak, Turgay
- Bioresource Technology, Vol. 155
Lipid Profile Remodeling in Response to Nitrogen Deprivation in the Microalgae Chlorella sp. (Trebouxiophyceae) and Nannochloropsis sp. (Eustigmatophyceae)
journal, August 2014
- Martin, Gregory J. O.; Hill, David R. A.; Olmstead, Ian L. D.
- PLoS ONE, Vol. 9, Issue 8
CXCR4 signaling directs Igk recombination and the molecular mechanisms of late B lymphopoiesis
journal, September 2019
- Mandal, Malay; Okoreeh, Michael K.; Kennedy, Domenick E.
- Nature Immunology, Vol. 20, Issue 10
Edible oils from microalgae: insights in TAG accumulation
journal, October 2014
- Klok, A. J.; Lamers, P. P.; Martens, D. E.
- Trends in Biotechnology, Vol. 32, Issue 10
Emiliania huxleyi endures N-limitation with an efficient metabolic budgeting and effective ATP synthesis
journal, January 2014
- Rokitta, Sebastian D.; Von Dassow, Peter; Rost, Björn
- BMC Genomics, Vol. 15, Issue 1
Functional study of diacylglycerol acyltransferase type 2 family in Chlamydomonas reinhardtii
journal, June 2013
- Hung, Chun-Hsien; Ho, Ming-Yang; Kanehara, Kazue
- FEBS Letters, Vol. 587, Issue 15
SortMeRNA: fast and accurate filtering of ribosomal RNAs in metatranscriptomic data
journal, October 2012
- Kopylova, Evguenia; Noé, Laurent; Touzet, Hélène
- Bioinformatics, Vol. 28, Issue 24
edgeR: a Bioconductor package for differential expression analysis of digital gene expression data
journal, November 2009
- Robinson, M. D.; McCarthy, D. J.; Smyth, G. K.
- Bioinformatics, Vol. 26, Issue 1
Rapid Triacylglycerol Turnover in Chlamydomonas reinhardtii Requires a Lipase with Broad Substrate Specificity
journal, October 2012
- Li, Xiaobo; Benning, Christoph; Kuo, Min-Hao
- Eukaryotic Cell, Vol. 11, Issue 12
Opposing T cell responses in experimental autoimmune encephalomyelitis
journal, August 2019
- Saligrama, Naresha; Zhao, Fan; Sikora, Michael J.
- Nature, Vol. 572, Issue 7770
Characteristics of lipid extraction from Chlorella sp. cultivated in outdoor raceway ponds with mixture of ethyl acetate and ethanol for biodiesel production
journal, September 2015
- Lu, Weidong; Wang, Zhongming; Yuan, Zhenhong
- Bioresource Technology, Vol. 191
Screening and phylogenetic analysis of lipid-rich microalgae
journal, September 2015
- Li, Ling; Cui, Jing; Liu, Qian
- Algal Research, Vol. 11
Changes in Transcript Abundance in Chlamydomonas reinhardtii following Nitrogen Deprivation Predict Diversion of Metabolism
journal, October 2010
- Miller, Rachel; Wu, Guangxi; Deshpande, Rahul R.
- Plant Physiology, Vol. 154, Issue 4
Biomass and lipid productivities of Chlorella vulgaris under autotrophic, heterotrophic and mixotrophic growth conditions
journal, March 2009
- Liang, Yanna; Sarkany, Nicolas; Cui, Yi
- Biotechnology Letters, Vol. 31, Issue 7
Transcriptome sequencing and annotation of the microalgae Dunaliella tertiolecta: Pathway description and gene discovery for production of next-generation biofuels
journal, March 2011
- Rismani-Yazdi, Hamid; Haznedaroglu, Berat Z.; Bibby, Kyle
- BMC Genomics, Vol. 12, Issue 1
Lipid accumulation and biosynthesis genes response of the oleaginous Chlorella pyrenoidosa under three nutrition stressors
journal, January 2014
- Fan, Jianhua; Cui, Yanbin; Wan, Minxi
- Biotechnology for Biofuels, Vol. 7, Issue 1
Corrigendum
journal, May 2004
- Berges, J. A.; Franklin, D. J.; Harrison, P. J.
- Journal of Phycology, Vol. 40, Issue 3, p. 619-619
Metabolic and cellular organization in evolutionarily diverse microalgae as related to biofuels production
journal, June 2013
- Hildebrand, Mark; Abbriano, Raffaela M.; Polle, Juergen EW
- Current Opinion in Chemical Biology, Vol. 17, Issue 3
Identifying reference genes with stable expression from high throughput sequence data
journal, January 2012
- Alexander, Harriet; Jenkins, Bethany D.; Rynearson, Tatiana A.
- Frontiers in Microbiology, Vol. 3
The effect of iron on growth, lipid accumulation, and gene expression profile of the freshwater microalga Chlorella sorokiniana
journal, September 2014
- Wan, Minxi; Jin, Xuejie; Xia, Jinlan
- Applied Microbiology and Biotechnology, Vol. 98, Issue 22
Draft genome sequence and genetic transformation of the oleaginous alga Nannochloropsis gaditana
journal, January 2012
- Radakovits, Randor; Jinkerson, Robert E.; Fuerstenberg, Susan I.
- Nature Communications, Vol. 3, Issue 1
Red versus green leaves: transcriptomic comparison of foliar senescence between two Prunus cerasifera genotypes
journal, February 2020
- Vangelisti, Alberto; Guidi, Lucia; Cavallini, Andrea
- Scientific Reports, Vol. 10, Issue 1
Pathways of Lipid Metabolism in Marine Algae, Co-Expression Network, Bottlenecks and Candidate Genes for Enhanced Production of EPA and DHA in Species of Chromista
journal, November 2013
- Mühlroth, Alice; Li, Keshuai; Røkke, Gunvor
- Marine Drugs, Vol. 11, Issue 11
Genomic Foundation of Starch-to-Lipid Switch in Oleaginous Chlorella spp.
journal, October 2015
- Fan, Jianhua; Ning, Kang; Zeng, Xiaowei
- Plant Physiology, Vol. 169, Issue 4
Transcriptome sequencing of the marine microalga, Chlorella pyrenoidosa (Chlorophyta), and analysis of carbonic anhydrase expression under salt stress
journal, September 2014
- Sun, Xue; Wang, Weiwei; Shen, Jia
- Botanica Marina, Vol. 57, Issue 5
Preparation and Chemistry of the Artificial Algal Culture Medium Aquil
journal, January 1989
- Price, Neil M.; Harrison, Gail I.; Hering, Janet G.
- Biological Oceanography, Vol. 6, Issue 5-6
Works referencing / citing this record:
A downstream box fusion allows stable accumulation of a bacterial cellulase in Chlamydomonas reinhardtii chloroplasts
journal, May 2018
- Richter, Lubna V.; Yang, Huijun; Yazdani, Mohammad
- Biotechnology for Biofuels, Vol. 11, Issue 1
Metabolomic profiles of tropical Chlorella species in response to physiological changes during nitrogen deprivation
journal, June 2018
- Vello, Vejeysri; Chu, Wan-Loy; Lim, Phaik-Eem
- Journal of Applied Phycology, Vol. 30, Issue 6
Molecular basis of autotrophic vs mixotrophic growth in Chlorella sorokiniana
journal, April 2018
- Cecchin, M.; Benfatto, S.; Griggio, F.
- Scientific Reports, Vol. 8, Issue 1
A downstream box fusion allows stable accumulation of a bacterial cellulase in Chlamydomonas reinhardtii chloroplasts
journal, May 2018
- Richter, Lubna V.; Yang, Huijun; Yazdani, Mohammad
- Biotechnology for Biofuels, Vol. 11, Issue 1