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Title: An analog filter approach to frequency domain fluorescence spectroscopy

The rate equations found in frequency domain fluorescence spectroscopy are the same as those found in electronics under analog filter theory. Laplace transform methods are a natural way to solve the equations, and the methods can provide solutions for arbitrary excitation functions. The fluorescence terms can be modeled as circuit components and cascaded with drive and detection electronics to produce a global transfer function. Electronics design tools such as Spicea can be used to model fluorescence problems. In applications, such as remote sensing, where detection electronics are operated at high gain and limited bandwidth, a global modeling of the entire system is important, since the filter terms of the drive and detection electronics affect the measured response of the fluorescence signals. Furthermore, the techniques described here can be used to separate signals from fast and slow fluorophores emitting into the same spectral band, and data collection can be greatly accelerated by means of a frequency comb driver waveform and appropriate signal processing of the response.
Authors:
 [1] ;  [1] ;  [1]
  1. National Security Technologies, LLC. (NSTec), Mercury, NV (United States)
Publication Date:
OSTI Identifier:
1241008
Report Number(s):
DOE/NV/25946--2452
Journal ID: ISSN 1053-0509
Grant/Contract Number:
AC52-06NA25946
Type:
Accepted Manuscript
Journal Name:
Journal of Fluorescence
Additional Journal Information:
Journal Volume: 25; Journal Issue: 6; Journal ID: ISSN 1053-0509
Publisher:
Springer
Research Org:
Nevada Test Site/National Security Technologies, LLC (United States)
Sponsoring Org:
USDOE NA Office of Nonproliferation and Verification Research and Development (NA-22)
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY fluorescence; frequency domain; fluorophore; analog filter