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Title: Assessment of Normal Variability in Peripheral Blood Gene Expression

Peripheral blood is representative of many systemic processes and is an ideal sample for expression profiling of diseases that have no known or accessible lesion. Peripheral blood is a complex mixture of cell types and some differences in peripheral blood gene expression may reflect the timing of sample collection rather than an underlying disease process. For this reason, it is important to assess study design factors that may cause variability in gene expression not related to what is being analyzed. Variation in the gene expression of circulating peripheral blood mononuclear cells (PBMCs) from three healthy volunteers sampled three times one day each week for one month was examined for 1,176 genes printed on filter arrays. Less than 1% of the genes showed any variation in expression that was related to the time of collection, and none of the changes were noted in more than one individual. These results suggest that observed variation was due to experimental variability.
Authors:
 [1] ;  [1] ;  [1] ;  [1] ;  [1]
  1. Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
Publication Date:
OSTI Identifier:
1239167
Type:
Published Article
Journal Name:
Disease Markers
Additional Journal Information:
Journal Volume: 18; Journal Issue: 4; Related Information: CHORUS Timestamp: 2016-08-23 05:13:46; Journal ID: ISSN 0278-0240
Publisher:
Hindawi Publishing Corporation
Sponsoring Org:
USDOE
Country of Publication:
Country unknown/Code not available
Language:
English