Biomass accessibility analysis using electron tomography

Hinkle, Jacob D.; Ciesielski, Peter N.; Gruchalla, Kenny; Munch, Kristin R.; Donohoe, Bryon S.

doi:10.1186/s13068-015-0395-8

Title: Biomass accessibility analysis using electron tomography

Abstract

Substrate accessibility to catalysts has been a dominant theme in theories of biomass deconstruction. Furthermore, current methods of quantifying accessibility do not elucidate mechanisms for increased accessibility due to changes in microstructure following pretreatment.

Authors:: Hinkle, Jacob D.; Ciesielski, Peter N.; Gruchalla, Kenny; Munch, Kristin R.; Donohoe, Bryon S.

Publication Date:: Fri Dec 25 00:00:00 EST 2015

Research Org.:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Basic Energy Sciences (BES); USDOE Office of Science (SC), Biological and Environmental Research (BER)

OSTI Identifier:: 1618628

Alternate Identifier(s):: OSTI ID: 1236164

Report Number(s):: NREL/JA-2C00-65701
Journal ID: ISSN 1754-6834; 212; PII: 395

Grant/Contract Number:: SC0000997; BESC; AC36-08GO28308

Resource Type:: Published Article

Journal Name:: Biotechnology for Biofuels

Additional Journal Information:: Journal Name: Biotechnology for Biofuels Journal Volume: 8 Journal Issue: 1; Journal ID: ISSN 1754-6834

Publisher:: Springer Science + Business Media

Country of Publication:: Netherlands

Language:: English

Subject:: 09 BIOMASS FUELS; accessibility; porosimetry; tomography; cellulose; pretreatment; biomass

Citation Formats


                    Hinkle, Jacob D., Ciesielski, Peter N., Gruchalla, Kenny, Munch, Kristin R., and Donohoe, Bryon S. Biomass accessibility analysis using electron tomography.  Netherlands: N. p., 2015. 
Web.  doi:10.1186/s13068-015-0395-8.

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                    Hinkle, Jacob D., Ciesielski, Peter N., Gruchalla, Kenny, Munch, Kristin R., & Donohoe, Bryon S. Biomass accessibility analysis using electron tomography.  Netherlands.  https://doi.org/10.1186/s13068-015-0395-8

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                    Hinkle, Jacob D., Ciesielski, Peter N., Gruchalla, Kenny, Munch, Kristin R., and Donohoe, Bryon S. Fri .  
"Biomass accessibility analysis using electron tomography".  Netherlands.  https://doi.org/10.1186/s13068-015-0395-8.

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@article{osti_1618628,

  title        = {Biomass accessibility analysis using electron tomography},

  author       = {Hinkle, Jacob D. and Ciesielski, Peter N. and Gruchalla, Kenny and Munch, Kristin R. and Donohoe, Bryon S.},

  abstractNote = {Substrate accessibility to catalysts has been a dominant theme in theories of biomass deconstruction. Furthermore, current methods of quantifying accessibility do not elucidate mechanisms for increased accessibility due to changes in microstructure following pretreatment.},

  doi          = {10.1186/s13068-015-0395-8},

  journal      = {Biotechnology for Biofuels},

  number       = 1,

  volume       = 8,

  place        = {Netherlands},

  year         = {Fri Dec 25 00:00:00 EST 2015},

  month        = {Fri Dec 25 00:00:00 EST 2015}

}

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Works referenced in this record:

Substrate and Enzyme Characteristics that Limit Cellulose Hydrolysis
journal, October 1999

Mansfield, S. D.; Mooney, C.; Saddler, J. N.
Biotechnology Progress, Vol. 15, Issue 5
DOI: 10.1021/bp9900864

Determination of the Pore Size of Cell Walls of Living Plant Cells
journal, September 1979

Carpita, N.; Sabularse, D.; Montezinos, D.
Science, Vol. 205, Issue 4411
DOI: 10.1126/science.205.4411.1144

Fungal cellulases and complexed cellulosomal enzymes exhibit synergistic mechanisms in cellulose deconstruction
journal, January 2013

Resch, Michael G.; Donohoe, Bryon S.; Baker, John O.
Energy & Environmental Science, Vol. 6, Issue 6
DOI: 10.1039/c3ee00019b

Dual-Axis Tomography: An Approach with Alignment Methods That Preserve Resolution
journal, December 1997

Mastronarde, David N.
Journal of Structural Biology, Vol. 120, Issue 3
DOI: 10.1006/jsbi.1997.3919

Cellulase digestibility of pretreated biomass is limited by cellulose accessibility
journal, January 2007

Jeoh, Tina; Ishizawa, Claudia I.; Davis, Mark F.
Biotechnology and Bioengineering, Vol. 98, Issue 1, p. 112-122
DOI: 10.1002/bit.21408

Do Enzymatic Hydrolyzability and Simons' Stain Reflect the Changes in the Accessibility of Lignocellulosic Substrates to Cellulase Enzymes?
journal, December 2001

Esteghlalian, A. R.; Bilodeau, M.; Mansfield, S. D.
Biotechnology Progress, Vol. 17, Issue 6
DOI: 10.1021/bp0101177

Dual-axis electron tomography: a new approach for investigating the spatial organization of wood cellulose microfibrils
journal, July 2006

Xu, Ping; Donaldson, Lloyd A.; Gergely, Zachary R.
Wood Science and Technology, Vol. 41, Issue 2
DOI: 10.1007/s00226-006-0088-3

Designing the deconstruction of plant cell walls
journal, June 2008

Mccann, M.; Carpita, N.
Current Opinion in Plant Biology, Vol. 11, Issue 3
DOI: 10.1016/j.pbi.2008.04.001

Optimal Separable Algorithms to Compute the Reverse Euclidean Distance Transformation and Discrete Medial Axis in Arbitrary Dimension
journal, March 2007

Coeurjolly, David; Montanvert, Annick
IEEE Transactions on Pattern Analysis and Machine Intelligence, Vol. 29, Issue 3
DOI: 10.1109/TPAMI.2007.54

Access of cellulase to cellulose and lignin for poplar solids produced by leading pretreatment technologies
journal, May 2009

Kumar, Rajeev; Wyman, Charles E.
Biotechnology Progress, Vol. 25, Issue 3
DOI: 10.1002/btpr.153

Growth of the plant cell wall
journal, November 2005

Cosgrove, Daniel J.
Nature Reviews Molecular Cell Biology, Vol. 6, Issue 11, p. 850-861
DOI: 10.1038/nrm1746

Features of promising technologies for pretreatment of lignocellulosic biomass
journal, April 2005

Mosier, Nathan; Wyman, Charles; Dale, Bruce
Bioresource Technology, Vol. 96, Issue 6, p. 673-686
DOI: 10.1016/j.biortech.2004.06.025

Revealing Nature's Cellulase Diversity The Digestion Mechanism of Caldicellulosiruptor bescii CelA
journal, December 2013

Brunecky, R.; Alahuhta, M.; Xu, Q.
Science, Vol. 342, Issue 6165, p. 1513-1516
DOI: 10.1126/science.1244273

Consolidated bioprocessing of cellulosic biomass: an update
journal, October 2005

Lynd, Lee R.; van Zyl, Willem H.; McBride, John E.
Current Opinion in Biotechnology, Vol. 16, Issue 5, p. 577-583
DOI: 10.1016/j.copbio.2005.08.009

Distance functions on digital pictures
journal, July 1968

Rosenfeld, A.; Pfaltz, J. L.
Pattern Recognition, Vol. 1, Issue 1
DOI: 10.1016/0031-3203(68)90013-7

A new method for the model‐independent assessment of thickness in three‐dimensional images
journal, January 1997

Hildebrand, T.; Rüegsegger, P.
Journal of Microscopy, Vol. 185, Issue 1
DOI: 10.1046/j.1365-2818.1997.1340694.x

Recent advances in understanding the role of cellulose accessibility in enzymatic hydrolysis of lignocellulosic substrates
journal, June 2014

Meng, Xianzhi; Ragauskas, Arthur Jonas
Current Opinion in Biotechnology, Vol. 27
DOI: 10.1016/j.copbio.2014.01.014

Porosity and Its Effect on the Digestibility of Dilute Sulfuric Acid Pretreated Corn Stover
journal, April 2007

Ishizawa, Claudia I.; Davis, Mark F.; Schell, Daniel F.
Journal of Agricultural and Food Chemistry, Vol. 55, Issue 7
DOI: 10.1021/jf062131a

Elucidating the role of ferrous ion cocatalyst in enhancing dilute acid pretreatment of lignocellulosic biomass
journal, January 2011

Wei, Hui; Donohoe, Bryon S.; Vinzant, Todd B.
Biotechnology for Biofuels, Vol. 4, Issue 1
DOI: 10.1186/1754-6834-4-48

Visualizing lignin coalescence and migration through maize cell walls following thermochemical pretreatment
journal, December 2008

Donohoe, Bryon S.; Decker, Stephen R.; Tucker, Melvin P.
Biotechnology and Bioengineering, Vol. 101, Issue 5
DOI: 10.1002/bit.21959

Use of X-ray tomography to study the porosity and morphology of granules
journal, May 2003

Farber, Leon; Tardos, Gabriel; Michaels, James N.
Powder Technology, Vol. 132, Issue 1
DOI: 10.1016/S0032-5910(03)00043-3

Computing contour trees in all dimensions
journal, February 2003

Carr, Hamish; Snoeyink, Jack; Axen, Ulrike
Computational Geometry, Vol. 24, Issue 2
DOI: 10.1016/S0925-7721(02)00093-7

Marching cubes: A high resolution 3D surface construction algorithm
journal, August 1987

Lorensen, William E.; Cline, Harvey E.
ACM SIGGRAPH Computer Graphics, Vol. 21, Issue 4
DOI: 10.1145/37402.37422

Characterization of Clostridium thermocellum JW20
journal, January 1988

Freier, Doris; Mothershed, Cheryle P.; Wiegel, Juergen
Applied and Environmental Microbiology, Vol. 54, Issue 1
DOI: 10.1128/AEM.54.1.204-211.1988

Robust Pore Size Analysis of Filamentous Networks from Three-Dimensional Confocal Microscopy
journal, December 2008

Mickel, Walter; Münster, Stefan; Jawerth, Louise M.
Biophysical Journal, Vol. 95, Issue 12
DOI: 10.1529/biophysj.108.135939

Automated electron microscope tomography using robust prediction of specimen movements
journal, October 2005

Mastronarde, David N.
Journal of Structural Biology, Vol. 152, Issue 1
DOI: 10.1016/j.jsb.2005.07.007

3D Electron Tomography of Pretreated Biomass Informs Atomic Modeling of Cellulose Microfibrils
journal, August 2013

Ciesielski, Peter N.; Matthews, James F.; Tucker, Melvin P.
ACS Nano, Vol. 7, Issue 9
DOI: 10.1021/nn4031542

Computer Visualization of Three-Dimensional Image Data Using IMOD
journal, January 1996

Kremer, James R.; Mastronarde, David N.; McIntosh, J. Richard
Journal of Structural Biology, Vol. 116, Issue 1
DOI: 10.1006/jsbi.1996.0013

Effect of mechanical disruption on the effectiveness of three reactors used for dilute acid pretreatment of corn stover Part 1: chemical and physical substrate analysis
journal, January 2014

Wang, Wei; Chen, Xiaowen; Donohoe, Bryon S.
Biotechnology for Biofuels, Vol. 7, Issue 1
DOI: 10.1186/1754-6834-7-57

Clean Fractionation Pretreatment Reduces Enzyme Loadings for Biomass Saccharification and Reveals the Mechanism of Free and Cellulosomal Enzyme Synergy
journal, May 2014

Resch, Michael G.; Donohoe, Bryon S.; Ciesielski, Peter N.
ACS Sustainable Chemistry & Engineering, Vol. 2, Issue 6
DOI: 10.1021/sc500210w

From Voxels to Knowledge: A Practical Guide to the Segmentation of Complex Electron Microscopy 3D-Data
journal, January 2014

Tsai, Wen-Ting; Hassan, Ahmed; Sarkar, Purbasha
Journal of Visualized Experiments, Issue 90
DOI: 10.3791/51673

Electron Tomography of Cryo-Immobilized Plant Tissue: A Novel Approach to Studying 3D Macromolecular Architecture of Mature Plant Cell Walls In Situ
journal, September 2014

Sarkar, Purbasha; Bosneaga, Elena; Yap, Edgar G.
PLoS ONE, Vol. 9, Issue 9
DOI: 10.1371/journal.pone.0106928

User-guided 3D active contour segmentation of anatomical structures: Significantly improved efficiency and reliability
journal, July 2006

Yushkevich, Paul A.; Piven, Joseph; Hazlett, Heather Cody
NeuroImage, Vol. 31, Issue 3
DOI: 10.1016/j.neuroimage.2006.01.015

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Title: Biomass accessibility analysis using electron tomography

Abstract

Citation Formats

Substrate and Enzyme Characteristics that Limit Cellulose Hydrolysis journal, October 1999

Determination of the Pore Size of Cell Walls of Living Plant Cells journal, September 1979

Fungal cellulases and complexed cellulosomal enzymes exhibit synergistic mechanisms in cellulose deconstruction journal, January 2013

Dual-Axis Tomography: An Approach with Alignment Methods That Preserve Resolution journal, December 1997

Cellulase digestibility of pretreated biomass is limited by cellulose accessibility journal, January 2007

Do Enzymatic Hydrolyzability and Simons' Stain Reflect the Changes in the Accessibility of Lignocellulosic Substrates to Cellulase Enzymes? journal, December 2001

Dual-axis electron tomography: a new approach for investigating the spatial organization of wood cellulose microfibrils journal, July 2006

Designing the deconstruction of plant cell walls journal, June 2008

Optimal Separable Algorithms to Compute the Reverse Euclidean Distance Transformation and Discrete Medial Axis in Arbitrary Dimension journal, March 2007

Access of cellulase to cellulose and lignin for poplar solids produced by leading pretreatment technologies journal, May 2009

Growth of the plant cell wall journal, November 2005

Features of promising technologies for pretreatment of lignocellulosic biomass journal, April 2005

Revealing Nature's Cellulase Diversity The Digestion Mechanism of Caldicellulosiruptor bescii CelA journal, December 2013

Consolidated bioprocessing of cellulosic biomass: an update journal, October 2005

Distance functions on digital pictures journal, July 1968

A new method for the model‐independent assessment of thickness in three‐dimensional images journal, January 1997

Recent advances in understanding the role of cellulose accessibility in enzymatic hydrolysis of lignocellulosic substrates journal, June 2014

Porosity and Its Effect on the Digestibility of Dilute Sulfuric Acid Pretreated Corn Stover journal, April 2007

Elucidating the role of ferrous ion cocatalyst in enhancing dilute acid pretreatment of lignocellulosic biomass journal, January 2011

Visualizing lignin coalescence and migration through maize cell walls following thermochemical pretreatment journal, December 2008

Use of X-ray tomography to study the porosity and morphology of granules journal, May 2003

Computing contour trees in all dimensions journal, February 2003

Marching cubes: A high resolution 3D surface construction algorithm journal, August 1987

Characterization of Clostridium thermocellum JW20 journal, January 1988

Robust Pore Size Analysis of Filamentous Networks from Three-Dimensional Confocal Microscopy journal, December 2008

Automated electron microscope tomography using robust prediction of specimen movements journal, October 2005

3D Electron Tomography of Pretreated Biomass Informs Atomic Modeling of Cellulose Microfibrils journal, August 2013

Computer Visualization of Three-Dimensional Image Data Using IMOD journal, January 1996

Effect of mechanical disruption on the effectiveness of three reactors used for dilute acid pretreatment of corn stover Part 1: chemical and physical substrate analysis journal, January 2014

Clean Fractionation Pretreatment Reduces Enzyme Loadings for Biomass Saccharification and Reveals the Mechanism of Free and Cellulosomal Enzyme Synergy journal, May 2014

From Voxels to Knowledge: A Practical Guide to the Segmentation of Complex Electron Microscopy 3D-Data journal, January 2014

Electron Tomography of Cryo-Immobilized Plant Tissue: A Novel Approach to Studying 3D Macromolecular Architecture of Mature Plant Cell Walls In Situ journal, September 2014

User-guided 3D active contour segmentation of anatomical structures: Significantly improved efficiency and reliability journal, July 2006

Substrate and Enzyme Characteristics that Limit Cellulose Hydrolysis
journal, October 1999

Determination of the Pore Size of Cell Walls of Living Plant Cells
journal, September 1979

Fungal cellulases and complexed cellulosomal enzymes exhibit synergistic mechanisms in cellulose deconstruction
journal, January 2013

Dual-Axis Tomography: An Approach with Alignment Methods That Preserve Resolution
journal, December 1997

Cellulase digestibility of pretreated biomass is limited by cellulose accessibility
journal, January 2007

Do Enzymatic Hydrolyzability and Simons' Stain Reflect the Changes in the Accessibility of Lignocellulosic Substrates to Cellulase Enzymes?
journal, December 2001

Dual-axis electron tomography: a new approach for investigating the spatial organization of wood cellulose microfibrils
journal, July 2006

Designing the deconstruction of plant cell walls
journal, June 2008

Optimal Separable Algorithms to Compute the Reverse Euclidean Distance Transformation and Discrete Medial Axis in Arbitrary Dimension
journal, March 2007

Access of cellulase to cellulose and lignin for poplar solids produced by leading pretreatment technologies
journal, May 2009

Growth of the plant cell wall
journal, November 2005

Features of promising technologies for pretreatment of lignocellulosic biomass
journal, April 2005

Revealing Nature's Cellulase Diversity The Digestion Mechanism of Caldicellulosiruptor bescii CelA
journal, December 2013

Consolidated bioprocessing of cellulosic biomass: an update
journal, October 2005

Distance functions on digital pictures
journal, July 1968

A new method for the model‐independent assessment of thickness in three‐dimensional images
journal, January 1997

Recent advances in understanding the role of cellulose accessibility in enzymatic hydrolysis of lignocellulosic substrates
journal, June 2014

Porosity and Its Effect on the Digestibility of Dilute Sulfuric Acid Pretreated Corn Stover
journal, April 2007

Elucidating the role of ferrous ion cocatalyst in enhancing dilute acid pretreatment of lignocellulosic biomass
journal, January 2011

Visualizing lignin coalescence and migration through maize cell walls following thermochemical pretreatment
journal, December 2008

Use of X-ray tomography to study the porosity and morphology of granules
journal, May 2003

Computing contour trees in all dimensions
journal, February 2003

Marching cubes: A high resolution 3D surface construction algorithm
journal, August 1987

Characterization of Clostridium thermocellum JW20
journal, January 1988

Robust Pore Size Analysis of Filamentous Networks from Three-Dimensional Confocal Microscopy
journal, December 2008

Automated electron microscope tomography using robust prediction of specimen movements
journal, October 2005

3D Electron Tomography of Pretreated Biomass Informs Atomic Modeling of Cellulose Microfibrils
journal, August 2013

Computer Visualization of Three-Dimensional Image Data Using IMOD
journal, January 1996

Effect of mechanical disruption on the effectiveness of three reactors used for dilute acid pretreatment of corn stover Part 1: chemical and physical substrate analysis
journal, January 2014

Clean Fractionation Pretreatment Reduces Enzyme Loadings for Biomass Saccharification and Reveals the Mechanism of Free and Cellulosomal Enzyme Synergy
journal, May 2014

From Voxels to Knowledge: A Practical Guide to the Segmentation of Complex Electron Microscopy 3D-Data
journal, January 2014

Electron Tomography of Cryo-Immobilized Plant Tissue: A Novel Approach to Studying 3D Macromolecular Architecture of Mature Plant Cell Walls In Situ
journal, September 2014

User-guided 3D active contour segmentation of anatomical structures: Significantly improved efficiency and reliability
journal, July 2006