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This content will become publicly available on January 14, 2017

Title: Combining Metabolic Engineering and Electrocatalysis. Application to the Production of Polyamides from Sugar

Biorefineries aim to convert biomass into a spectrum of products ranging from biofuels to specialty chemicals. To achieve economically sustainable conversion, it is crucial to streamline the catalytic and downstream processing steps. In this work, a route that combines bio- and electrocatalysis to convert glucose into bio-based unsaturated nylon-6,6 is reported. An engineered strain of Saccharomyces cerevisiae was used as the initial biocatalyst for the conversion of glucose into muconic acid, with the highest reported muconic acid titer of 559.5 mg L-1 in yeast. Without any separation, muconic acid was further electrocatalytically hydrogenated to 3-hexenedioic acid in 94 % yield despite the presence of biogenic impurities. Bio-based unsaturated nylon-6,6 (unsaturated polyamide-6,6) was finally obtained by polymerization of 3-hexenedioic acid with hexamethylenediamine.
Authors:
 [1] ;  [2] ;  [1] ;  [3] ;  [4] ;  [4] ;  [3] ;  [1] ;  [2]
  1. Iowa State Univ., Ames, IA (United States); NSF Engineering Research Center for Biorenewable Chemicals (CBiRC), Ames, IA (United States)
  2. Iowa State Univ., Ames, IA (United States); NSF Engineering Research Center for Biorenewable Chemicals (CBiRC), Ames, IA (United States); Ames Lab., Ames, IA (United States)
  3. Iowa State Univ., Ames, IA (United States)
  4. NSF Engineering Research Center for Biorenewable Chemicals (CBiRC), Ames, IA (United States)
Publication Date:
OSTI Identifier:
1235228
Report Number(s):
IS--J-8730
Journal ID: ISSN 1433-7851
Grant/Contract Number:
EEC-0813570; EPSC-1101284; AC02-07CH11358
Type:
Accepted Manuscript
Journal Name:
Angewandte Chemie (International Edition)
Additional Journal Information:
Journal Name: Angewandte Chemie (International Edition); Journal Volume: 55; Journal Issue: 7; Journal ID: ISSN 1433-7851
Publisher:
Wiley
Research Org:
Ames Laboratory (AMES), Ames, IA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES