Comparison of Internal Energy Distributions of Ions Created by Electrospray Ionization and Laser Ablation-Liquid Vortex Capture-Electrospray Ionization

Cahill, John F.; Kertesz, Vilmos; Ovchinnikova, Olga S.; Van Berkel, Gary J.

doi:10.1007/s13361-015-1195-x

Title: Comparison of Internal Energy Distributions of Ions Created by Electrospray Ionization and Laser Ablation-Liquid Vortex Capture-Electrospray Ionization

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Abstract

Recently a number of techniques have combined laser ablation with liquid capture for mass spectrometry spot sampling and imaging applications. The newly developed non-contact liquid-vortex capture probe has been used to efficiently collect 355 nm UV laser ablated material in a continuous flow solvent stream in which the captured material dissolves and then undergoes electrospray ionization. This sampling and ionization approach has produced what appear to be classic electrospray ionization spectra; however, the softness of this sampling/ionization process versus simple electrospray ionization has not been definitely determined. A series of benzlypyridinium salts, known as thermometer ions, were used to compare internal energy distributions between electrospray ionization and the UV laser ablation liquid-vortex capture probe electrospray combination. Measured internal energy distributions were identical between the two techniques, even with differences in laser fluence (0.7-3.1 J cm-2) and when using UV-absorbing or non-UV-absorbing sample substrates. This data indicates ions formed directly by UV laser ablation, if any, are likely an extremely small constituent of the total ion signal observed. Instead, neutral molecules, clusters or particulates ejected from the surface during laser ablation, subsequently captured and dissolved in the flowing solvent stream then electrosprayed are the predominant source of ion signal observed. Themore »« less

Authors:

Cahill, John F. ^[1]; Kertesz, Vilmos ^[1]; Ovchinnikova, Olga S. ^[1]; Van Berkel, Gary J. ^[1]

Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Publication Date:: Sat Jun 27 00:00:00 EDT 2015

Research Org.:: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Sponsoring Org.:: USDOE Office of Science (SC)

OSTI Identifier:: 1235006

Grant/Contract Number:: AC05-00OR22725

Resource Type:: Accepted Manuscript

Journal Name:: Journal of the American Society for Mass Spectrometry

Additional Journal Information:: Journal Volume: 26; Journal Issue: 9; Journal ID: ISSN 1044-0305

Publisher:: American Society for Mass Spectrometry

Country of Publication:: United States

Language:: English

Subject:: 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; Laser ablation Liquid capture Electrospray ionization Thermometer ions Internal energy Surface sampling Mass spectrometry imaging

Citation Formats


                    Cahill, John F., Kertesz, Vilmos, Ovchinnikova, Olga S., and Van Berkel, Gary J. Comparison of Internal Energy Distributions of Ions Created by Electrospray Ionization and Laser Ablation-Liquid Vortex Capture-Electrospray Ionization.  United States: N. p., 2015. 
Web.  doi:10.1007/s13361-015-1195-x.

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                    Cahill, John F., Kertesz, Vilmos, Ovchinnikova, Olga S., & Van Berkel, Gary J. Comparison of Internal Energy Distributions of Ions Created by Electrospray Ionization and Laser Ablation-Liquid Vortex Capture-Electrospray Ionization.  United States.  https://doi.org/10.1007/s13361-015-1195-x

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                    Cahill, John F., Kertesz, Vilmos, Ovchinnikova, Olga S., and Van Berkel, Gary J. Sat .  
"Comparison of Internal Energy Distributions of Ions Created by Electrospray Ionization and Laser Ablation-Liquid Vortex Capture-Electrospray Ionization".  United States.  https://doi.org/10.1007/s13361-015-1195-x.  https://www.osti.gov/servlets/purl/1235006.

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@article{osti_1235006,

  title        = {Comparison of Internal Energy Distributions of Ions Created by Electrospray Ionization and Laser Ablation-Liquid Vortex Capture-Electrospray Ionization},

  author       = {Cahill, John F. and Kertesz, Vilmos and Ovchinnikova, Olga S. and Van Berkel, Gary J.},

  abstractNote = {Recently a number of techniques have combined laser ablation with liquid capture for mass spectrometry spot sampling and imaging applications. The newly developed non-contact liquid-vortex capture probe has been used to efficiently collect 355 nm UV laser ablated material in a continuous flow solvent stream in which the captured material dissolves and then undergoes electrospray ionization. This sampling and ionization approach has produced what appear to be classic electrospray ionization spectra; however, the softness of this sampling/ionization process versus simple electrospray ionization has not been definitely determined. A series of benzlypyridinium salts, known as thermometer ions, were used to compare internal energy distributions between electrospray ionization and the UV laser ablation liquid-vortex capture probe electrospray combination. Measured internal energy distributions were identical between the two techniques, even with differences in laser fluence (0.7-3.1 J cm-2) and when using UV-absorbing or non-UV-absorbing sample substrates. This data indicates ions formed directly by UV laser ablation, if any, are likely an extremely small constituent of the total ion signal observed. Instead, neutral molecules, clusters or particulates ejected from the surface during laser ablation, subsequently captured and dissolved in the flowing solvent stream then electrosprayed are the predominant source of ion signal observed. The electrospray ionization process used controls the softness of the technique.},

  doi          = {10.1007/s13361-015-1195-x},

  journal      = {Journal of the American Society for Mass Spectrometry},

  number       = 9,

  volume       = 26,

  place        = {United States},

  year         = {Sat Jun 27 00:00:00 EDT 2015},

  month        = {Sat Jun 27 00:00:00 EDT 2015}

}

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