Exploiting algal NADPH oxidase for biophotovoltaic energy
Abstract
Photosynthetic microbes exhibit light-dependent electron export across the cell membrane, which can generate electricity in biological photovoltaic (BPV) devices. How electrons are exported remains to be determined; the identification of mechanisms would help selection or generation of photosynthetic microbes capable of enhanced electrical output. We show that plasma membrane NADPH oxidase activity is a significant component of light-dependent generation of electricity by the unicellular green alga Chlamydomonas reinhardtii. NADPH oxidases export electrons across the plasma membrane to form superoxide anion from oxygen. The C. reinhardtii mutant lacking the NADPH oxidase encoded by RBO1 is impaired in both extracellular superoxide anion production and current generation in a BPV device. Complementation with the wild-type gene restores both capacities, demonstrating the role of the enzyme in electron export. Monitoring light-dependent extracellular superoxide production with a colorimetric assay is shown to be an effective way of screening for electrogenic potential of candidate algal strains. The results show that algal NADPH oxidases are important for superoxide anion production and open avenues for optimizing the biological component of these devices.
- Authors:
-
- Department of Plant Sciences University of Cambridge Cambridge UK
- Department of Chemistry and Biochemistry UCLA Los Angeles CA USA
- Department of Biochemistry University of Cambridge Cambridge UK
- Institute for Genomics and Proteomics UCLA Los Angeles CA USA
- Publication Date:
- Research Org.:
- Univ. of Cambridge, Cambridge (United Kingdom); Univ. of California, Los Angeles, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1234170
- Alternate Identifier(s):
- OSTI ID: 1234171; OSTI ID: 1363910; OSTI ID: 1438189
- Grant/Contract Number:
- DE–FC02–02ER63421; FC02-02ER63421
- Resource Type:
- Published Article
- Journal Name:
- Plant Biotechnology Journal
- Additional Journal Information:
- Journal Name: Plant Biotechnology Journal Journal Volume: 14 Journal Issue: 1; Journal ID: ISSN 1467-7644
- Publisher:
- Wiley-Blackwell
- Country of Publication:
- United Kingdom
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; alga; biophotovoltaic; Chlamydomonas; energy; NADPH oxidase; Chlamydomonas, energy
Citation Formats
Anderson, Alexander, Laohavisit, Anuphon, Blaby, Ian K., Bombelli, Paolo, Howe, Christopher J., Merchant, Sabeeha S., Davies, Julia M., and Smith, Alison G. Exploiting algal NADPH oxidase for biophotovoltaic energy. United Kingdom: N. p., 2015.
Web. doi:10.1111/pbi.12332.
Anderson, Alexander, Laohavisit, Anuphon, Blaby, Ian K., Bombelli, Paolo, Howe, Christopher J., Merchant, Sabeeha S., Davies, Julia M., & Smith, Alison G. Exploiting algal NADPH oxidase for biophotovoltaic energy. United Kingdom. https://doi.org/10.1111/pbi.12332
Anderson, Alexander, Laohavisit, Anuphon, Blaby, Ian K., Bombelli, Paolo, Howe, Christopher J., Merchant, Sabeeha S., Davies, Julia M., and Smith, Alison G. Thu .
"Exploiting algal NADPH oxidase for biophotovoltaic energy". United Kingdom. https://doi.org/10.1111/pbi.12332.
@article{osti_1234170,
title = {Exploiting algal NADPH oxidase for biophotovoltaic energy},
author = {Anderson, Alexander and Laohavisit, Anuphon and Blaby, Ian K. and Bombelli, Paolo and Howe, Christopher J. and Merchant, Sabeeha S. and Davies, Julia M. and Smith, Alison G.},
abstractNote = {Photosynthetic microbes exhibit light-dependent electron export across the cell membrane, which can generate electricity in biological photovoltaic (BPV) devices. How electrons are exported remains to be determined; the identification of mechanisms would help selection or generation of photosynthetic microbes capable of enhanced electrical output. We show that plasma membrane NADPH oxidase activity is a significant component of light-dependent generation of electricity by the unicellular green alga Chlamydomonas reinhardtii. NADPH oxidases export electrons across the plasma membrane to form superoxide anion from oxygen. The C. reinhardtii mutant lacking the NADPH oxidase encoded by RBO1 is impaired in both extracellular superoxide anion production and current generation in a BPV device. Complementation with the wild-type gene restores both capacities, demonstrating the role of the enzyme in electron export. Monitoring light-dependent extracellular superoxide production with a colorimetric assay is shown to be an effective way of screening for electrogenic potential of candidate algal strains. The results show that algal NADPH oxidases are important for superoxide anion production and open avenues for optimizing the biological component of these devices.},
doi = {10.1111/pbi.12332},
journal = {Plant Biotechnology Journal},
number = 1,
volume = 14,
place = {United Kingdom},
year = {Thu Jan 29 00:00:00 EST 2015},
month = {Thu Jan 29 00:00:00 EST 2015}
}
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