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Title: Characterization of Fatty Acids in Crenarchaeota by GC-MS and NMR

Lipids composed of condensed isoprenyl units connected to glycerol backbones by ether linkages are a distinguishing feature of Archaea. Data suggesting that fatty acids with linear hydrocarbon chains are present in some Archaea have been available for decades. However, lack of genomic and biochemical evidence for the metabolic machinery required to synthesize and degrade fatty acids has left the field unclear on this potentially significant biochemical aspect. Because lipids are energy currency and cell signaling molecules, their presence in Archaea is significant for understanding archaeal biology. A recent large-scale bioinformatics analysis reignited the debate as to the importance of fatty acids in Archaea by presenting genetic evidence for the presence of enzymes required for anabolic and catabolic fatty acid metabolism across the archaeal domain. Here, we present direct biochemical evidence from gas chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR) spectroscopy for the presence of fatty acids in two members of the Crenarchaeota, Sulfolobus solfataricus and Ignicoccus hospitalis . This is the first report providing biochemical data for the existence of fatty acids in these Crenarchaeota, opening new discussions on energy balance and the potential for the discovery of new thermostable enzymes for industry.
Authors:
 [1] ;  [1] ;  [2] ;  [3] ;  [2] ;  [4] ;  [4]
  1. Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT 59717, USA
  2. Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA, Department of Microbiology, University of Tennessee, Knoxville, TN 37996, USA
  3. Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA
  4. Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT 59717, USA, Thermal Biology Institute, Montana State University, Bozeman, MT 59717, USA
Publication Date:
OSTI Identifier:
1234125
Grant/Contract Number:
SC0006654
Type:
Published Article
Journal Name:
Archaea
Additional Journal Information:
Journal Volume: 2015; Related Information: CHORUS Timestamp: 2016-08-18 16:03:48; Journal ID: ISSN 1472-3646
Publisher:
Hindawi Publishing Corporation
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English