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Title: Lipidomic Analysis of Endocannabinoid Signaling: Targeted Metabolite Identification and Quantification

The endocannabinoids N -arachidonoylethanolamide (or anandamide, AEA) and 2-arachidonoylglycerol (2-AG) belong to the larger groups of N -acylethanolamines (NAEs) and monoacylglycerol (MAG) lipid classes, respectively. They are biologically active lipid molecules that activate G-protein-coupled cannabinoid receptors found in various organisms. After AEA and 2-AG were discovered in the 1990s, they have been extensively documented to have a broad range of physiological functions. Along with AEA, several NAEs, for example, N -palmitoylethanolamine (PEA), N -stearoylethanolamine (SEA), and N -oleoylethanolamine (OEA) are also present in tissues, usually at much larger concentrations than AEA. Any perturbation that involves the endocannabinoid pathway may subsequently alter basal level or metabolism of these lipid mediators. Further, the altered levels of these molecules often reflect pathological conditions associated with tissue damage. Robust and sensitive methodologies to analyze these lipid mediators are essential to understanding how they act as endocannabinoids. The recent advances in mass spectrometry allow researchers to develop lipidomics approaches and several methodologies have been proposed to quantify endocannabinoids in various biological systems.
Authors:
 [1] ;  [2]
  1. Department of Biological Sciences, Center for Plant Lipid Research, University of North Texas, Denton, TX 76203, USA, Brookhaven National Laboratory, 50 Bell Avenue, Building 463, P.O. Box 5000, Upton, NY 11973-5000, USA
  2. Department of Biological Sciences, Center for Plant Lipid Research, University of North Texas, Denton, TX 76203, USA
Publication Date:
OSTI Identifier:
1234050
Grant/Contract Number:
FG02-05ER15647
Type:
Published Article
Journal Name:
Neural Plasticity
Additional Journal Information:
Journal Volume: 2016; Related Information: CHORUS Timestamp: 2016-07-26 09:06:16; Journal ID: ISSN 2090-5904
Publisher:
Hindawi Publishing Corporation
Sponsoring Org:
USDOE
Country of Publication:
Country unknown/Code not available
Language:
English