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Title: Structure of a Rhamnogalacturonan Fragment from Apple Pectin: Implications for Pectin Architecture

A commercial apple pectin was sequentially digested with the cloned enzymes endopolygalacturonase, galactanase, arabinofuranosidase, xylogalacturonase, and rhamnogalacturonan hydrolase. The rhamnogalacturonan hydrolase-generated oligosaccharides were separated by ultrafiltration, anion exchange, and size-exclusion chromatography. Fractions from the ion exchange chromatography were pooled, lyophilized, and screened by MALDI-TOF MS. An oligosaccharide (RGP14P3) was identified and its structure, α -D-Gal p A- ( 1 2 ) - α -L-Rha p - ( 1 4 ) - α -D-Gal p A- ( 1 2 ) - α -L-Rha p - ( 1 4 ) - α -D-Gal p A, determined by 1D and 2D NMR spectrometry. This oligosaccharide probably represents a direct connection between homogalacturonan and rhamnogalacturonan in pectin. Alternatively, it could indicate that the nonreducing end of rhamnogalacturonan starts with a galacturonic acid residue.
Authors:
 [1] ;  [1]
  1. Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078-3035, USA
Publication Date:
OSTI Identifier:
1227787
Grant/Contract Number:
FG02-96ER20215
Type:
Published Article
Journal Name:
International Journal of Carbohydrate Chemistry
Additional Journal Information:
Journal Volume: 2014; Related Information: CHORUS Timestamp: 2016-07-28 16:59:17; Journal ID: ISSN 1687-9341
Publisher:
Hindawi Publishing Corporation
Sponsoring Org:
USDOE
Country of Publication:
Country unknown/Code not available
Language:
English