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Title: Immunoglobulin λ Gene Rearrangement Can Precede κ Gene Rearrangement

Immunoglobulin genes are generated during differentiation of B lymphocytes by joining gene segments. A mouse pre-B cell contains a functional immunoglobulin heavy-chain gene, but no light-chain gene. Although there is only one heavy-chain locus, there are two lightchain loci: κ and λ .It has been reported that κ loci in the germ-line configuration are never (in man) or very rarely (in the mouse) present in cells with functionally rearranged λ -chain genes. Two explanations have been proposed to explain this: (a) the ordered rearrangement theory, which postulates that light-chain gene rearrangement in the pre-B cell is first attempted at the κ locus, and that only upon failure to produce a functional κ chain is there an attempt to rearrange the λ locus; and (b) the stochastic theory, which postulates that rearrangement at the λ locus proceeds at a rate that is intrinsically much slower than that at the κ locus. We show here that λ -chain genes are generated whether or not the κ locus has lost its germ-line arrangement, a result that is compatible only with the stochastic theory.
Authors:
 [1] ;  [1] ;  [1] ;  [1]
  1. Department of Microbiology and Immulology, University of California, San Francisco, California 94143-0414, USA
Publication Date:
OSTI Identifier:
1227786
Grant/Contract Number:
AC03-76-SF01012
Type:
Published Article
Journal Name:
Developmental Immunology
Additional Journal Information:
Journal Volume: 1; Journal Issue: 1; Related Information: CHORUS Timestamp: 2016-08-22 13:02:33; Journal ID: ISSN 1044-6672
Publisher:
Hindawi Publishing Corporation
Sponsoring Org:
USDOE
Country of Publication:
Country unknown/Code not available
Language:
English