Title: Screening of Viral Pathogens from Pediatric Ileal Tissue Samples after Vaccination

Abstract

In 2010, researchers reported that the two US-licensed rotavirus vaccines contained DNA or DNA fragments from porcine circovirus (PCV). Although PCV, a common virus among pigs, is not thought to cause illness in humans, these findings raised several safety concerns. In this study, we sought to determine whether viruses, including PCV, could be detected in ileal tissue samples of children vaccinated with one of the two rotavirus vaccines. A broad spectrum, novel DNA detection technology, the Lawrence Livermore Microbial Detection Array (LLMDA), was utilized, and confirmation of viral pathogens using the polymerase chain reaction (PCR) was conducted. The LLMDA technology was recently used to identify PCV from one rotavirus vaccine. Ileal tissue samples were analyzed from 21 subjects, aged 15–62 months. PCV was not detected in any ileal tissue samples by the LLMDA or PCR. LLMDA identified a human rotavirus A from one of the vaccinated subjects, which is likely due to a recent infection from a wild type rotavirus. LLMDA also identified human parechovirus, a common gastroenteritis viral infection, from two subjects. Additionally, LLMDA detected common gastrointestinal bacterial organisms from the Enterobacteriaceae , Bacteroidaceae , and Streptococcaceae families from several subjects. This study provides a survey of viral andmore » bacterial pathogens from pediatric ileal samples, and may shed light on future studies to identify pathogen associations with pediatric vaccinations.« less

Authors:

Hewitson, Laura  ^[1];

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Thissen, James B. ^[2]; Gardner, Shea N. ^[3]; McLoughlin, Kevin S. ^[3]; Glausser, Margaret K. ^[4]; Jaing, Crystal J. ^[2]

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+ Show Author Affiliations

1. The Johnson Center for Child Health and Development, 1700 Rio Grande Street, Austin, TX 78701, USA, Department of Psychiatry, University of Texas Southwestern, Dallas, TX 75390, USA
2. Physical & Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA 94550, USA
3. Computations Directorate, Lawrence Livermore National Laboratory, Livermore, CA 94550, USA
4. The Johnson Center for Child Health and Development, 1700 Rio Grande Street, Austin, TX 78701, USA

Publication Date:

Wed Jan 01 00:00:00 EST 2014

Research Org.:

Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Org.:

USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division

OSTI Identifier:

1227761

Alternate Identifier(s):

OSTI ID: 1629045

Resource Type:

Published Article

Journal Name:

Advances in Virology

Additional Journal Information:

Journal Name: Advances in Virology Journal Volume: 2014; Journal ID: ISSN 1687-8639

Publisher:

Hindawi Publishing Corporation

Country of Publication:

United Kingdom

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES