Subcellular-level resolution MALDI-MS imaging of maize leaf metabolites by MALDI-linear ion trap-Orbitrap mass spectrometer
Abstract
A significant limiting factor in achieving high spatial resolution for matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) imaging is the size of the laser spot at the sample surface. We present modifications to the beam-delivery optics of a commercial MALDI-linear ion trap-Orbitrap instrument, incorporating an external Nd:YAG laser, beam-shaping optics, and an aspheric focusing lens, to reduce the minimum laser spot size from ~50 μm for the commercial configuration down to ~9 μm for the modified configuration. This improved system was applied for MALDI-MS imaging of cross sections of juvenile maize leaves at 5-μm spatial resolution using an oversampling method. There are a variety of different metabolites including amino acids, glycerolipids, and defense-related compounds were imaged at a spatial resolution well below the size of a single cell. Such images provide unprecedented insights into the metabolism associated with the different tissue types of the maize leaf, which is known to asymmetrically distribute the reactions of C4 photosynthesis among the mesophyll and bundle sheath cell types. The metabolite ion images correlate with the optical images that reveal the structures of the different tissues, and previously known and newly revealed asymmetric metabolic features are observed.
- Authors:
-
- Iowa State Univ., Ames, IA (United States); Ames Lab., Ames, IA (United States)
- Iowa State Univ., Ames, IA (United States)
- Publication Date:
- Research Org.:
- Ames Laboratory (AMES), Ames, IA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1226917
- Report Number(s):
- IS-J 8571
Journal ID: ISSN 1618-2642; PII: 8460
- Grant/Contract Number:
- AC02-07CH11358
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Analytical and Bioanalytical Chemistry
- Additional Journal Information:
- Journal Volume: 407; Journal Issue: 8; Journal ID: ISSN 1618-2642
- Publisher:
- Springer
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 47 OTHER INSTRUMENTATION; 59 BASIC BIOLOGICAL SCIENCES; mass spectrometry imaging; metabolite; maize
Citation Formats
Korte, Andrew R., Yandeau-Nelson, Marna D., Nikolau, Basil J., and Lee, Young Jin. Subcellular-level resolution MALDI-MS imaging of maize leaf metabolites by MALDI-linear ion trap-Orbitrap mass spectrometer. United States: N. p., 2015.
Web. doi:10.1007/s00216-015-8460-5.
Korte, Andrew R., Yandeau-Nelson, Marna D., Nikolau, Basil J., & Lee, Young Jin. Subcellular-level resolution MALDI-MS imaging of maize leaf metabolites by MALDI-linear ion trap-Orbitrap mass spectrometer. United States. https://doi.org/10.1007/s00216-015-8460-5
Korte, Andrew R., Yandeau-Nelson, Marna D., Nikolau, Basil J., and Lee, Young Jin. Sun .
"Subcellular-level resolution MALDI-MS imaging of maize leaf metabolites by MALDI-linear ion trap-Orbitrap mass spectrometer". United States. https://doi.org/10.1007/s00216-015-8460-5. https://www.osti.gov/servlets/purl/1226917.
@article{osti_1226917,
title = {Subcellular-level resolution MALDI-MS imaging of maize leaf metabolites by MALDI-linear ion trap-Orbitrap mass spectrometer},
author = {Korte, Andrew R. and Yandeau-Nelson, Marna D. and Nikolau, Basil J. and Lee, Young Jin},
abstractNote = {A significant limiting factor in achieving high spatial resolution for matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) imaging is the size of the laser spot at the sample surface. We present modifications to the beam-delivery optics of a commercial MALDI-linear ion trap-Orbitrap instrument, incorporating an external Nd:YAG laser, beam-shaping optics, and an aspheric focusing lens, to reduce the minimum laser spot size from ~50 μm for the commercial configuration down to ~9 μm for the modified configuration. This improved system was applied for MALDI-MS imaging of cross sections of juvenile maize leaves at 5-μm spatial resolution using an oversampling method. There are a variety of different metabolites including amino acids, glycerolipids, and defense-related compounds were imaged at a spatial resolution well below the size of a single cell. Such images provide unprecedented insights into the metabolism associated with the different tissue types of the maize leaf, which is known to asymmetrically distribute the reactions of C4 photosynthesis among the mesophyll and bundle sheath cell types. The metabolite ion images correlate with the optical images that reveal the structures of the different tissues, and previously known and newly revealed asymmetric metabolic features are observed.},
doi = {10.1007/s00216-015-8460-5},
journal = {Analytical and Bioanalytical Chemistry},
number = 8,
volume = 407,
place = {United States},
year = {Sun Jan 25 00:00:00 EST 2015},
month = {Sun Jan 25 00:00:00 EST 2015}
}
Web of Science
Works referenced in this record:
Compound and metabolite distribution measured by MALDI mass spectrometric imaging in whole-body tissue sections
journal, February 2007
- Stoeckli, Markus; Staab, Dieter; Schweitzer, Alain
- International Journal of Mass Spectrometry, Vol. 260, Issue 2-3
Sublimation as a method of matrix application for mass spectrometric imaging
journal, September 2007
- Hankin, Joseph A.; Barkley, Robert M.; Murphy, Robert C.
- Journal of the American Society for Mass Spectrometry, Vol. 18, Issue 9
High-Spatial and High-Mass Resolution Imaging of Surface Metabolites of Arabidopsis thaliana by Laser Desorption-Ionization Mass Spectrometry Using Colloidal Silver
journal, April 2010
- Jun, Ji Hyun; Song, Zhihong; Liu, Zhenjiu
- Analytical Chemistry, Vol. 82, Issue 8
Imaging MALDI Mass Spectrometry Using an Oscillating Capillary Nebulizer Matrix Coating System and Its Application to Analysis of Lipids in Brain from a Mouse Model of Tay−Sachs/Sandhoff Disease
journal, April 2008
- Chen, Yanfeng; Allegood, Jeremy; Liu, Ying
- Analytical Chemistry, Vol. 80, Issue 8
Dynamics of ions of intact proteins in the Orbitrap mass analyzer
journal, August 2009
- Makarov, Alexander; Denisov, Eduard
- Journal of the American Society for Mass Spectrometry, Vol. 20, Issue 8
Mass Spectrometric Imaging for Biomedical Tissue Analysis
journal, May 2010
- Chughtai, Kamila; Heeren, Ron M. A.
- Chemical Reviews, Vol. 110, Issue 5
MALDI Mass Spectrometry Imaging in Microscope Mode with Infrared Lasers: Bypassing the Diffraction Limits
journal, December 2013
- Soltwisch, Jens; Göritz, Guido; Jungmann, Julia H.
- Analytical Chemistry, Vol. 86, Issue 1
Scanning microprobe matrix-assisted laser desorption ionization (SMALDI) mass spectrometry: Instrumentation for sub-micrometer resolved LDI and MALDI surface analysis
journal, June 2002
- Spengler, Bernhard; Hubert, Martin
- Journal of the American Society for Mass Spectrometry, Vol. 13, Issue 6
Histology by Mass Spectrometry: Label-Free Tissue Characterization Obtained from High-Accuracy Bioanalytical Imaging
journal, April 2010
- Römpp, Andreas; Guenther, Sabine; Schober, Yvonne
- Angewandte Chemie International Edition, Vol. 49, Issue 22
Matrix-free UV-laser desorption/ionization (LDI) mass spectrometric imaging at the single-cell level: distribution of secondary metabolites of Arabidopsis thaliana and Hypericum species: Matrix-free UV-LDI mass spectrometric imaging at the single-cell level
journal, December 2009
- Hölscher, Dirk; Shroff, Rohit; Knop, Katrin
- The Plant Journal, Vol. 60, Issue 5
Laser Beam Filtration for High Spatial Resolution MALDI Imaging Mass Spectrometry
journal, May 2013
- Zavalin, Andre; Yang, Junhai; Caprioli, Richard
- Journal of The American Society for Mass Spectrometry, Vol. 24, Issue 7
Implementation of a Gaussian Beam Laser and Aspheric Optics for High Spatial Resolution MALDI Imaging MS
journal, April 2014
- Zavalin, Andre; Yang, Junhai; Haase, Andreas
- Journal of The American Society for Mass Spectrometry, Vol. 25, Issue 6
MALDI Mass Spectrometry Imaging of Bioactive Lipids in Mouse Brain with a Synapt G2-S Mass Spectrometer Operated at Elevated Pressure: Improving the Analytical Sensitivity and the Lateral Resolution to Ten Micrometers
journal, July 2014
- Kettling, Hans; Vens-Cappell, Simeon; Soltwisch, Jens
- Analytical Chemistry, Vol. 86, Issue 15
Targeted Multiplex Imaging Mass Spectrometry in Transmission Geometry for Subcellular Spatial Resolution
journal, February 2013
- Thiery-Lavenant, Gwendoline; Zavalin, Andre I.; Caprioli, Richard M.
- Journal of The American Society for Mass Spectrometry, Vol. 24, Issue 4
Direct imaging of single cells and tissue at sub-cellular spatial resolution using transmission geometry MALDI MS: Single-cell imaging with transmission geometry
journal, November 2012
- Zavalin, Andre; Todd, Erik M.; Rawhouser, Patrick D.
- Journal of Mass Spectrometry, Vol. 47, Issue 11
MALDI-MS imaging of features smaller than the size of the laser beam
journal, October 2005
- Jurchen, John C.; Rubakhin, Stanislav S.; Sweedler, Jonathan V.
- Journal of the American Society for Mass Spectrometry, Vol. 16, Issue 10
Detection of phosphoenolpyruvate and ribulose 1,5-bisphosphate carboxylase transcripts in maize leaves by in situ hybridization with sulfonated cDNA probes.
journal, April 1989
- Perrot-Rechenmann, C.; Joannes, M.; Squalli, D.
- Journal of Histochemistry & Cytochemistry, Vol. 37, Issue 4
Functional Differentiation of Bundle Sheath and Mesophyll Maize Chloroplasts Determined by Comparative Proteomics
journal, October 2005
- Majeran, Wojciech; Cai, Yang; Sun, Qi
- The Plant Cell, Vol. 17, Issue 11
MALDI-MS analysis and imaging of small molecule metabolites with 1,5-diaminonaphthalene (DAN): Diaminonaphthalene as small molecule MALDI matrix
journal, July 2014
- Korte, Andrew R.; Lee, Young Jin
- Journal of Mass Spectrometry, Vol. 49, Issue 8
Multiplex MALDI-MS Imaging of Plant Metabolites Using a Hybrid MS System
book, October 2014
- Korte, Andrew R.; Yagnik, Gargey B.; Feenstra, Adam D.
- Mass Spectrometry Imaging of Small Molecules
MALDI produced ions inspected with a linear ion trap-orbitrap hybrid mass analyzer
journal, August 2009
- Strupat, Kerstin; Kovtoun, Viatcheslav; Bui, Huy
- Journal of the American Society for Mass Spectrometry, Vol. 20, Issue 8
Determination of flavone, flavonol, and flavanone aglycones by negative ion liquid chromatography electrospray ion trap mass spectrometry
journal, June 2001
- Fabre, Nicolas; Rustan, Isabelle; Hoffmann, Edmond
- Journal of the American Society for Mass Spectrometry, Vol. 12, Issue 6
Phloem Loading: How Leaves Gain Their Independence
journal, January 2006
- Turgeon, Robert
- BioScience, Vol. 56, Issue 1
The Phenylpropanoid Pathway in Arabidopsis
journal, January 2011
- Fraser, Christopher M.; Chapple, Clint
- The Arabidopsis Book, Vol. 9
Effect of Maysin on Wild-Type, Deltamethrin-Resistant, and Bt-Resistant Helicoverpa armigera (Lepidoptera: Noctuidae)
journal, June 2003
- Rector, Brian G.; Liang, Gemei; Guo, Yuyuan
- Journal of Economic Entomology, Vol. 96, Issue 3
Differential accumulation of maysin and rhamnosylisoorientin in leaves of high-altitude landraces of maize after UV-B exposure
journal, June 2005
- Casati, Paula; Walbot, Virginia
- Plant, Cell and Environment, Vol. 28, Issue 6
Characterization of a flavonoid 3-O-glucosyltransferase and its activity during cotyledon growth in buckwheat (Fagopyrum esculentum)
journal, November 2005
- Suzuki, Tatsuro; Kim, Sun-Ju; Yamauchi, Hiroaki
- Plant Science, Vol. 169, Issue 5
Roles of the acidic lipids sulfoquinovosyl diacylglycerol and phosphatidylglycerol in photosynthesis: their specificity and evolution
journal, November 2004
- Sato, Norihiro
- Journal of Plant Research, Vol. 117, Issue 6
Phosphatidylglycerol and Chilling Sensitivity in Plants
journal, March 1985
- Roughan, P. Grattan
- Plant Physiology, Vol. 77, Issue 3
The Fatty Acid Composition of Phosphatidylglycerol and Sulfoquinovosyldiacylglycerol of Higher Plants in Relation to Chilling Sensitivity
journal, August 1986
- Kenrick, Janette R.; Bishop, David G.
- Plant Physiology, Vol. 81, Issue 4
Lipid molecular species composition of thylakoid membranes
journal, January 1980
- Nishihara, Masateru; Yokota, Kazushige; Kito, Makoto
- Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, Vol. 617, Issue 1
Induction and detoxification of maize 1,4-benzoxazin-3-ones by insect herbivores: Defense induction and detoxification in maize
journal, October 2011
- Glauser, Gaétan; Marti, Guillaume; Villard, Neil
- The Plant Journal, Vol. 68, Issue 5
Analysis of a Chemical Plant Defense Mechanism in Grasses
journal, August 1997
- Frey, M.
- Science, Vol. 277, Issue 5326
Natural Variation in Maize Aphid Resistance Is Associated with 2,4-Dihydroxy-7-Methoxy-1,4-Benzoxazin-3-One Glucoside Methyltransferase Activity
journal, June 2013
- Meihls, L. N.; Handrick, V.; Glauser, G.
- The Plant Cell, Vol. 25, Issue 6
Role of natural benzoxazinones in the survival strategy of plants
book, January 2000
- Sicker, Dieter; Frey, Monika; Schulz, Margot
- International Review of Cytology
What role does sulpholipid play within the thylakoid membrane?
journal, January 1986
- Barber, James; Gounaris, Kleoniki
- Photosynthesis Research, Vol. 9, Issue 1-2
What role does sulpholipid play within the thylakoid membrane?
book, January 1986
- Barber, James; Gounaris, Kleoniki
- Current topics in photosynthesis
Effect of Maysin on Wild-Type, Deltamethrin-Resistant, and Bt-Resistant Helicoverpa armigera (Lepidoptera: Noctuidae)
journal, June 2003
- Rector, Brian G.; Liang, Gemei; Guo, Yuyuan
- Journal of Economic Entomology, Vol. 96, Issue 3
Natural Variation in Maize Aphid Resistance Is Associated with 2,4-Dihydroxy-7-Methoxy-1,4-Benzoxazin-3-One Glucoside Methyltransferase Activity
text, January 2013
- Glauser, G.; Jander, G.; Kollner, T. G.
- American Society of Plant Biologists
Direct imaging of single cells and tissue at sub-cellular spatial resolution using transmission geometry MALDI MS
journal, November 2012
- Zavalin, Andre; Todd, Erik M.; Rawhouser, Patrick D.
- Journal of Mass Spectrometry, Vol. 47, Issue 11
Works referencing / citing this record:
Study of the cyanoacrylate fuming mechanism by matrix-assisted laser desorption/ionization mass spectrometry
journal, February 2019
- O'Neill, Kelly C.; Lee, Young Jin
- Journal of Mass Spectrometry, Vol. 54, Issue 3
Recent advances in matrix-assisted laser desorption/ionisation mass spectrometry imaging (MALDI-MSI) for in situ analysis of endogenous molecules in plants
journal, April 2018
- Qin, Liang; Zhang, Yawen; Liu, Yaqin
- Phytochemical Analysis, Vol. 29, Issue 4
Confirmation of sub-cellular resolution using oversampling imaging mass spectrometry
journal, November 2019
- Maimó-Barceló, Albert; Garate, Jone; Bestard-Escalas, Joan
- Analytical and Bioanalytical Chemistry, Vol. 411, Issue 30
Evaluation of lipid coverage and high spatial resolution MALDI-imaging capabilities of oversampling combined with laser post-ionisation
journal, December 2019
- Bowman, Andrew P.; Bogie, Jeroen F. J.; Hendriks, Jerome J. A.
- Analytical and Bioanalytical Chemistry, Vol. 412, Issue 10
Mass spectrometry imaging for plant biology: a review
journal, October 2015
- Boughton, Berin A.; Thinagaran, Dinaiz; Sarabia, Daniel
- Phytochemistry Reviews, Vol. 15, Issue 3
Plant defense and herbivore counter-defense: benzoxazinoids and insect herbivores
journal, November 2016
- Wouters, Felipe C.; Blanchette, Blair; Gershenzon, Jonathan
- Phytochemistry Reviews, Vol. 15, Issue 6
Metabotyping of 30 maize hybrids under early-sowing conditions reveals potential marker-metabolites for breeding
journal, September 2018
- Lamari, Nadia; Zhendre, Vanessa; Urrutia, Maria
- Metabolomics, Vol. 14, Issue 10
Keeping the shape of plant tissue for visualizing metabolite features in segmentation and correlation analysis of imaging mass spectrometry in Asparagus officinalis
journal, February 2019
- Nakabayashi, Ryo; Hashimoto, Kei; Toyooka, Kiminori
- Metabolomics, Vol. 15, Issue 2
microMS: A Python Platform for Image-Guided Mass Spectrometry Profiling
journal, June 2017
- Comi, Troy J.; Neumann, Elizabeth K.; Do, Thanh D.
- Journal of The American Society for Mass Spectrometry, Vol. 28, Issue 9
3D MALDI Mass Spectrometry Imaging of a Single Cell: Spatial Mapping of Lipids in the Embryonic Development of Zebrafish
journal, November 2017
- Dueñas, Maria Emilia; Essner, Jeffrey J.; Lee, Young Jin
- Scientific Reports, Vol. 7, Issue 1
Subcellular Peptide Localization in Single Identified Neurons by Capillary Microsampling Mass Spectrometry
journal, August 2018
- Zhang, Linwen; Khattar, Nikkita; Kemenes, Ildiko
- Scientific Reports, Vol. 8, Issue 1
Assimilation of ‘omics’ strategies to study the cuticle layer and suberin lamellae in plants
journal, October 2017
- Cohen, Hagai; Szymanski, Jedrzej; Aharoni, Asaph
- Journal of Experimental Botany, Vol. 68, Issue 19
Chemical Imaging of Cyanoacrylate-Fumed Fingerprints by Matrix-assisted Laser Desorption/Ionization Mass Spectrometry Imaging,
journal, March 2018
- O'Neill, Kelly C.; Hinners, Paige; Lee, Young Jin
- Journal of Forensic Sciences, Vol. 63, Issue 6
Biological tissue sample preparation for time-of-flight secondary ion mass spectrometry (ToF–SIMS) imaging
journal, September 2018
- Yoon, Sohee; Lee, Tae Geol
- Nano Convergence, Vol. 5, Issue 1
Applications of Fourier Transform Ion Cyclotron Resonance (FT-ICR) and Orbitrap Based High Resolution Mass Spectrometry in Metabolomics and Lipidomics
journal, May 2016
- Ghaste, Manoj; Mistrik, Robert; Shulaev, Vladimir
- International Journal of Molecular Sciences, Vol. 17, Issue 6
Mass spectrometry imaging for plant biology: a review
journal, October 2015
- Boughton, Berin A.; Thinagaran, Dinaiz; Sarabia, Daniel
- Phytochemistry Reviews, Vol. 15, Issue 3
Plant defense and herbivore counter-defense: benzoxazinoids and insect herbivores
journal, November 2016
- Wouters, Felipe C.; Blanchette, Blair; Gershenzon, Jonathan
- Phytochemistry Reviews, Vol. 15, Issue 6
Ultra-High Mass Resolving Power, Mass Accuracy, and Dynamic Range MALDI Mass Spectrometry Imaging by 21-T FT-ICR MS
journal, January 2020
- Bowman, Andrew P.; Blakney, Greg T.; Hendrickson, Christopher L.
- Analytical Chemistry, Vol. 92, Issue 4
3D MALDI Mass Spectrometry Imaging of a Single Cell: Spatial Mapping of Lipids in the Embryonic Development of Zebrafish
journal, November 2017
- Dueñas, Maria Emilia; Essner, Jeffrey J.; Lee, Young Jin
- Scientific Reports, Vol. 7, Issue 1
Biological tissue sample preparation for time-of-flight secondary ion mass spectrometry (ToF–SIMS) imaging
journal, September 2018
- Yoon, Sohee; Lee, Tae Geol
- Nano Convergence, Vol. 5, Issue 1
Sample Preparation for Mass Spectrometry Imaging of Plant Tissues: A Review
journal, February 2016
- Dong, Yonghui; Li, Bin; Malitsky, Sergey
- Frontiers in Plant Science, Vol. 7
Comparison of Vacuum MALDI and AP-MALDI Platforms for the Mass Spectrometry Imaging of Metabolites Involved in Salt Stress in Medicago truncatula
journal, August 2018
- Keller, Caitlin; Maeda, Junko; Jayaraman, Dhileepkumar
- Frontiers in Plant Science, Vol. 9
Toward Mass Spectrometry Imaging in the Metabolomics Scale: Increasing Metabolic Coverage Through Multiple On-Tissue Chemical Modifications
journal, July 2019
- Dueñas, Maria Emilia; Larson, Evan A.; Lee, Young Jin
- Frontiers in Plant Science, Vol. 10
Applications of Fourier Transform Ion Cyclotron Resonance (FT-ICR) and Orbitrap Based High Resolution Mass Spectrometry in Metabolomics and Lipidomics
journal, May 2016
- Ghaste, Manoj; Mistrik, Robert; Shulaev, Vladimir
- International Journal of Molecular Sciences, Vol. 17, Issue 6