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Title: Structural and functional characterization of the hazelnut allergen Cor a 8

Nonspecific lipid transfer proteins (nsLTPs) are basic proteins, stabilized by four disulfide bonds, and are expressed throughout the plant kingdom. These proteins are also known as important allergens in fruits and tree nuts. In this study, the nsLTP from hazelnuts, Cor a 8, was purified and its crystal structure determined. The protein is stable at low pH and refolds after thermal denaturation. Molecular dynamics simulations were used to provide an insight into conformational changes of Cor a 8 upon ligand binding. When known epitope areas from Pru p 3 were compared to those of Cor a 8, differences were obvious, which may contribute to limited cross-reactivity between peach and hazelnut allergens. The differences in epitope regions may contribute to limited cross-reactivity between Cor a 8 and nsLTPs from other plant sources. The structure of Cor a 8 represents the first resolved structure of a hazelnut allergen.
Authors:
 [1] ;  [2] ;  [3] ;  [2] ;  [2] ;  [4] ;  [3] ;  [2]
  1. Univ. of South Carolina, Columbia, SC (United States); Davidson College, Davidson, NC (United States)
  2. Univ. of Vienna, Vienna (Austria)
  3. Univ. of South Carolina, Columbia, SC (United States)
  4. Polish Academy of Sciences, Krakow (Poland)
Publication Date:
OSTI Identifier:
1224206
Grant/Contract Number:
AC02-06CH11357; W-31-109-ENG-38; SFB F4603; W1248
Type:
Accepted Manuscript
Journal Name:
Journal of Agricultural and Food Chemistry
Additional Journal Information:
Journal Volume: 63; Journal Issue: 41; Journal ID: ISSN 0021-8561
Publisher:
American Chemical Society
Research Org:
Argonne National Laboratory (ANL), Argonne, IL (United States)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES allergen; food allergy; hazelnut; nonspecific lipid transfer protein; protein crystallization