skip to main content

DOE PAGESDOE PAGES

Title: Goniometer-based femtosecond crystallography with X-ray free electron lasers

The emerging method of femtosecond crystallography (FX) may extend the diffraction resolution accessible from small radiation-sensitive crystals and provides a means to determine catalytically accurate structures of acutely radiation-sensitive metalloenzymes. Automated goniometer-based instrumentation developed for use at the Linac Coherent Light Source enabled efficient and flexible FX experiments to be performed on a variety of sample types. In the case of rod-shaped Cpl hydrogenase crystals, only five crystals and about 30 min of beam time were used to obtain the 125 still diffraction patterns used to produce a 1.6-Å resolution electron density map. With smaller crystals, high-density grids were used to increase sample throughput; 930 myoglobin crystals mounted at random orientation inside 32 grids were exposed, demonstrating the utility of this approach. Screening results from cryocooled crystals of β2-adrenoreceptor and an RNA polymerase II complex indicate the potential to extend the diffraction resolution obtainable from very radiation-sensitive samples beyond that possible with undulator-based synchrotron sources.
Authors:
 [1] ;  [1] ;  [1] ;  [1] ;  [1] ;  [2] ;  [3] ;  [1] ;  [4] ;  [5] ;  [2] ;  [1] ;  [1] ;  [1] ;  [1] ;  [1] ;  [4] ;  [1] ;  [1] ;  [6] more »;  [7] ;  [5] ;  [1] ;  [5] ;  [1] ;  [2] ;  [5] ;  [5] ;  [1] ;  [1] ;  [1] ;  [7] ;  [4] ;  [1] ;  [1] ;  [2] ;  [3] ;  [5] ;  [1] ;  [3] ;  [5] ;  [7] ;  [3] ;  [1] ;  [3] « less
  1. SLAC National Accelerator Lab., Menlo Park, CA (United States)
  2. Univ. of Pittsburgh, PA (United States)
  3. SLAC National Accelerator Lab., Menlo Park, CA (United States); Stanford Univ., CA (United States)
  4. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  5. Stanford Univ., CA (United States)
  6. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Univ. of California, San Francisco, CA (United States)
  7. Montana State Univ., Bozeman, MT (United States)
Publication Date:
OSTI Identifier:
1221828
Grant/Contract Number:
AC02-05CH11231; P41GM103393; GM102520; GM095887; GM073210; GM082250; GM094625; HHMI Collaborative Innovation Award (HCIA)
Type:
Accepted Manuscript
Journal Name:
Proceedings of the National Academy of Sciences of the United States of America
Additional Journal Information:
Journal Volume: 111; Journal Issue: 48; Journal ID: ISSN 0027-8424
Publisher:
National Academy of Sciences, Washington, DC (United States)
Research Org:
Argonne National Laboratory (ANL), Argonne, IL (United States)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
Country of Publication:
United States
Language:
English
Subject:
femtosecond diffraction; crystallography; XFEL; structural biology