skip to main content

DOE PAGESDOE PAGES

Title: E-cadherin junction formation involves an active kinetic nucleation process

Epithelial (E)-cadherin-mediated cell–cell junctions play important roles in the development and maintenance of tissue structure in multicellular organisms. E-cadherin adhesion is thus a key element of the cellular microenvironment that provides both mechanical and biochemical signaling inputs. Here, we report in vitro reconstitution of junction-like structures between native E-cadherin in living cells and the extracellular domain of E-cadherin in a supported membrane. Junction formation in this hybrid live cell-supported membrane configuration requires both active processes within the living cell and a supported membrane with low E-cad-ECD mobility. The hybrid junctions recruit α-catenin and exhibit remodeled cortical actin. Observations suggest that the initial stages of junction formation in this hybrid system depend on the trans but not the cis interactions between E-cadherin molecules, and proceed via a nucleation process in which protrusion and retraction of filopodia play a key role.
Authors:
 [1] ;  [2] ;  [1] ;  [3] ;  [3] ;  [4] ;  [4] ;  [4] ;  [1] ;  [1] ;  [5] ;  [6] ;  [7] ;  [7] ;  [1] ;  [8]
  1. National Univ. of Singapore (Singapore)
  2. National Univ. of Singapore (Singapore); Univ. of California, Berkeley, CA (United States)
  3. Columiba Univ., New York, NY (United States)
  4. Univ. of California, Berkeley, CA (United States)
  5. Northwestern Univ., Chicago, IL (United States)
  6. Univ. of Oxford, Headington (United Kingdom)
  7. Columbia Univ., New York, NY (United States)
  8. National Univ. of Singapore (Singapore); Univ. of California, Berkeley, CA (United States); Lawrence Berkeley National Lab., Berkeley, CA (United States)
Publication Date:
OSTI Identifier:
1221818
Grant/Contract Number:
AC02-05CH11231; MCB-1412472; CRP001-084; NRF-RF2009-RF001-074; 100262/Z/12/Z; PRF #100262/Z/12/Z; R01 GM062270; AR44016
Type:
Accepted Manuscript
Journal Name:
Proceedings of the National Academy of Sciences of the United States of America
Additional Journal Information:
Journal Volume: 112; Journal Issue: 35; Journal ID: ISSN 0027-8424
Publisher:
National Academy of Sciences, Washington, DC (United States)
Research Org:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES cadherin; diffusion; adhesion; nucleation; bilayer