Growth of Chlamydomonas reinhardtii in acetate-free medium when co-cultured with alginate-encapsulated, acetate-producing strains of Synechococcus sp. PCC 7002

Therien, Jesse B.; Zadvornyy, Oleg A.; Posewitz, Matthew C.; Bryant, Donald A.; Peters, John W.

doi:10.1186/s13068-014-0154-2

Title: Growth of Chlamydomonas reinhardtii in acetate-free medium when co-cultured with alginate-encapsulated, acetate-producing strains of Synechococcus sp. PCC 7002

Abstract

The model alga Chlamydomonas reinhardtii requires acetate as a co-substrate for optimal production of lipids, and the addition of acetate to culture media has practical and economic implications for algal biofuel production. We demonstrate the growth of C. reinhardtii on acetate provided by mutant strains of the cyanobacterium Synechococcus sp. PCC7002.

Authors:

Therien, Jesse B. ^[1]; Zadvornyy, Oleg A. ^[1]; Posewitz, Matthew C. ^[2]; Bryant, Donald A. ^[3]; Peters, John W. ^[1]

Montana State Univ., Bozeman, MT (United States)
Colorado School of Mines, Golden, CO (United States)
Montana State Univ., Bozeman, MT (United States); The Pennsylvania State Univ., University Park, PA (United States)

Publication Date:: Sat Oct 18 00:00:00 EDT 2014

Research Org.:: Montana State Univ., Bozeman, MT (United States)

Sponsoring Org.:: USDOE Office of Energy Efficiency and Renewable Energy (EERE)

OSTI Identifier:: 1221648

Grant/Contract Number:: EE0003136

Resource Type:: Accepted Manuscript

Journal Name:: Biotechnology for Biofuels

Additional Journal Information:: Journal Volume: 7; Journal Issue: 1; Journal ID: ISSN 1754-6834

Publisher:: BioMed Central

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS; biofuels; algae; cyanobacteria; lipid production; acetate production; co-culture; alginate immobilization

Citation Formats


                    Therien, Jesse B., Zadvornyy, Oleg A., Posewitz, Matthew C., Bryant, Donald A., and Peters, John W. Growth of Chlamydomonas reinhardtii in acetate-free medium when co-cultured with alginate-encapsulated, acetate-producing strains of Synechococcus sp. PCC 7002.  United States: N. p., 2014. 
Web.  doi:10.1186/s13068-014-0154-2.

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                    Therien, Jesse B., Zadvornyy, Oleg A., Posewitz, Matthew C., Bryant, Donald A., & Peters, John W. Growth of Chlamydomonas reinhardtii in acetate-free medium when co-cultured with alginate-encapsulated, acetate-producing strains of Synechococcus sp. PCC 7002.  United States.  https://doi.org/10.1186/s13068-014-0154-2

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                    Therien, Jesse B., Zadvornyy, Oleg A., Posewitz, Matthew C., Bryant, Donald A., and Peters, John W. Sat .  
"Growth of Chlamydomonas reinhardtii in acetate-free medium when co-cultured with alginate-encapsulated, acetate-producing strains of Synechococcus sp. PCC 7002".  United States.  https://doi.org/10.1186/s13068-014-0154-2.  https://www.osti.gov/servlets/purl/1221648.

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@article{osti_1221648,

  title        = {Growth of Chlamydomonas reinhardtii in acetate-free medium when co-cultured with alginate-encapsulated, acetate-producing strains of Synechococcus sp. PCC 7002},

  author       = {Therien, Jesse B. and Zadvornyy, Oleg A. and Posewitz, Matthew C. and Bryant, Donald A. and Peters, John W.},

  abstractNote = {The model alga Chlamydomonas reinhardtii requires acetate as a co-substrate for optimal production of lipids, and the addition of acetate to culture media has practical and economic implications for algal biofuel production. We demonstrate the growth of C. reinhardtii on acetate provided by mutant strains of the cyanobacterium Synechococcus sp. PCC7002.},

  doi          = {10.1186/s13068-014-0154-2},

  journal      = {Biotechnology for Biofuels},

  number       = 1,

  volume       = 7,

  place        = {United States},

  year         = {Sat Oct 18 00:00:00 EDT 2014},

  month        = {Sat Oct 18 00:00:00 EDT 2014}

}

Copy to clipboard

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https://doi.org/10.1186/s13068-014-0154-2

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Cited by: 23 works

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Genetic engineering of fatty acid chain length in Phaeodactylum tricornutum
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Radakovits, Randor; Eduafo, Patrick M.; Posewitz, Matthew C.
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Synechococcus sp. Strain PCC 7002 Transcriptome: Acclimation to Temperature, Salinity, Oxidative Stress, and Mixotrophic Growth Conditions
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Ludwig, Marcus; Bryant, Donald A.
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Determination of Unknown Concentrations of Sodium Acetate Using the Method of Standard Addition and Proton NMR: An Experiment for the Undergraduate Analytical Chemistry Laboratory
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Demonstration of sustained hydrogen photoproduction by immobilized, sulfur-deprived Chlamydomonas reinhardtii cells
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Altered carbohydrate metabolism in glycogen synthase mutants of Synechococcus sp. strain PCC 7002: Cell factories for soluble sugars
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Xu, Yu; Tiago Guerra, L.; Li, Zhongkui
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Second Generation Biofuels: High-Efficiency Microalgae for Biodiesel Production
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Starchless Mutants of Chlamydomonas reinhardtii Lack the Small Subunit of a Heterotetrameric ADP-Glucose Pyrophosphorylase
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Altered carbohydrate metabolism in glycogen synthase mutants of Synechococcus sp. strain PCC 7002: Cell factories for soluble sugars
journal, March 2013

Xu, Yu; Tiago Guerra, L.; Li, Zhongkui
Metabolic Engineering, Vol. 16
DOI: 10.1016/j.ymben.2012.12.002

Demonstration of sustained hydrogen photoproduction by immobilized, sulfur-deprived Chlamydomonas reinhardtii cells
journal, April 2006

Laurinavichene, T.; Fedorov, A.; Ghirardi, M.
International Journal of Hydrogen Energy, Vol. 31, Issue 5
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Life cycle assessment of biodiesel production from microalgae in ponds
journal, January 2011

Campbell, Peter K.; Beer, Tom; Batten, David
Bioresource Technology, Vol. 102, Issue 1
DOI: 10.1016/j.biortech.2010.06.048

Hydrogen production by sulfur-deprived Chlamydomonas reinhardtii under photoautotrophic conditions
journal, September 2006

Tsygankov, A.; Kosourov, S.; Tolstygina, I.
International Journal of Hydrogen Energy, Vol. 31, Issue 11
DOI: 10.1016/j.ijhydene.2006.06.024

Phenotypic diversity of hydrogen production in chlorophycean algae reflects distinct anaerobic metabolisms
journal, June 2009

Meuser, Jonathan E.; Ananyev, Gennady; Wittig, Lauren E.
Journal of Biotechnology, Vol. 142, Issue 1
DOI: 10.1016/j.jbiotec.2009.01.015

Redirecting Reductant Flux into Hydrogen Production via Metabolic Engineering of Fermentative Carbon Metabolism in a Cyanobacterium
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McNeely, K.; Xu, Y.; Bennette, N.
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journal, June 2009

Posten, Clemens; Schaub, Georg
Journal of Biotechnology, Vol. 142, Issue 1
DOI: 10.1016/j.jbiotec.2009.03.015

Determination of Unknown Concentrations of Sodium Acetate Using the Method of Standard Addition and Proton NMR: An Experiment for the Undergraduate Analytical Chemistry Laboratory
journal, August 2012

Rajabzadeh, Massy
Journal of Chemical Education, Vol. 89, Issue 11
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A quick isolation method for mutants with high lipid yield in oleaginous yeast
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Works referencing / citing this record:

Alignment of microbial fitness with engineered product formation: obligatory coupling between acetate production and photoautotrophic growth
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Biotechnology for Biofuels, Vol. 11, Issue 1
DOI: 10.1186/s13068-018-1037-8

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journal, May 2018

Hu, Zhan; Qi, Yun; Zhao, Liu
Waste and Biomass Valorization, Vol. 10, Issue 12
DOI: 10.1007/s12649-018-0325-7

Lauric Acid Production in a Glycogen-Less Strain of Synechococcus sp. PCC 7002
journal, April 2015

Work, Victoria H.; Melnicki, Matthew R.; Hill, Eric A.
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DOI: 10.1007/s10811-017-1326-9

Mimicking lichens: incorporation of yeast strains together with sucrose-secreting cyanobacteria improves survival, growth, ROS removal, and lipid production in a stable mutualistic co-culture production platform
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Title: Growth of Chlamydomonas reinhardtii in acetate-free medium when co-cultured with alginate-encapsulated, acetate-producing strains of Synechococcus sp. PCC 7002

Abstract

Citation Formats

Genetic engineering of fatty acid chain length in Phaeodactylum tricornutum journal, January 2011

Biogenic hydrogen and methane production from Chlorella vulgaris and Dunaliella tertiolecta biomass journal, January 2011

Genetic engineering of fatty acid chain length in Phaeodactylum tricornutum journal, January 2011

Co-culturing a novel Bacillus strain with Clostridium tyrobutyricum ATCC 25755 to produce butyric acid from sucrose journal, January 2013

Biofuels from algae: challenges and potential journal, September 2010

Biodiesel production by simultaneous extraction and conversion of total lipids from microalgae, cyanobacteria, and wild mixed-cultures journal, February 2011

Biofuels from algae: challenges and potential journal, September 2010

Biogenic hydrogen and methane production from Chlorella vulgaris and Dunaliella tertiolecta biomass journal, January 2011

Co-culturing a novel Bacillus strain with Clostridium tyrobutyricum ATCC 25755 to produce butyric acid from sucrose journal, January 2013

Synechococcus sp. Strain PCC 7002 Transcriptome: Acclimation to Temperature, Salinity, Oxidative Stress, and Mixotrophic Growth Conditions journal, January 2012

Determination of Unknown Concentrations of Sodium Acetate Using the Method of Standard Addition and Proton NMR: An Experiment for the Undergraduate Analytical Chemistry Laboratory journal, August 2012

Increased Lipid Accumulation in the Chlamydomonas reinhardtii sta7-10 Starchless Isoamylase Mutant and Increased Carbohydrate Synthesis in Complemented Strains journal, June 2010

Hydrogen production by sulfur-deprived Chlamydomonas reinhardtii under photoautotrophic conditions journal, September 2006

Engineering algae for biohydrogen and biofuel production journal, June 2009

Regulatory branch points affecting protein and lipid biosynthesis in the diatom Phaeodactylum tricornutum journal, December 2013

Microalgae and terrestrial biomass as source for fuels—A process view journal, June 2009

Biodiesel production by simultaneous extraction and conversion of total lipids from microalgae, cyanobacteria, and wild mixed-cultures journal, February 2011

Demonstration of sustained hydrogen photoproduction by immobilized, sulfur-deprived Chlamydomonas reinhardtii cells journal, April 2006

Altered carbohydrate metabolism in glycogen synthase mutants of Synechococcus sp. strain PCC 7002: Cell factories for soluble sugars journal, March 2013

Second Generation Biofuels: High-Efficiency Microalgae for Biodiesel Production journal, March 2008

Starchless Mutants of Chlamydomonas reinhardtii Lack the Small Subunit of a Heterotetrameric ADP-Glucose Pyrophosphorylase journal, February 2001

Altered carbohydrate metabolism in glycogen synthase mutants of Synechococcus sp. strain PCC 7002: Cell factories for soluble sugars journal, March 2013

Demonstration of sustained hydrogen photoproduction by immobilized, sulfur-deprived Chlamydomonas reinhardtii cells journal, April 2006

Life cycle assessment of biodiesel production from microalgae in ponds journal, January 2011

Hydrogen production by sulfur-deprived Chlamydomonas reinhardtii under photoautotrophic conditions journal, September 2006

Phenotypic diversity of hydrogen production in chlorophycean algae reflects distinct anaerobic metabolisms journal, June 2009

Redirecting Reductant Flux into Hydrogen Production via Metabolic Engineering of Fermentative Carbon Metabolism in a Cyanobacterium journal, June 2010

NUTRITIONAL STUDIES WITH CHLAMYDOMONAS REINHARDI journal, October 1953

Microalgae and terrestrial biomass as source for fuels—A process view journal, June 2009

Determination of Unknown Concentrations of Sodium Acetate Using the Method of Standard Addition and Proton NMR: An Experiment for the Undergraduate Analytical Chemistry Laboratory journal, August 2012

A quick isolation method for mutants with high lipid yield in oleaginous yeast journal, January 2009

Engineering algae for biohydrogen and biofuel production journal, June 2009

The Production of Hydrogen Peroxide by Blue-Green Algae: a Survey journal, December 1973

Alignment of microbial fitness with engineered product formation: obligatory coupling between acetate production and photoautotrophic growth journal, February 2018

Mimicking lichens: incorporation of yeast strains together with sucrose-secreting cyanobacteria improves survival, growth, ROS removal, and lipid production in a stable mutualistic co-culture production platform journal, March 2017

Alignment of microbial fitness with engineered product formation: obligatory coupling between acetate production and photoautotrophic growth journal, February 2018

Interactions Between Microalgae and Microorganisms for Wastewater Remediation and Biofuel Production journal, May 2018

Lauric Acid Production in a Glycogen-Less Strain of Synechococcus sp. PCC 7002 journal, April 2015

Metabolomic and physiological changes of Chlamydomonas reinhardtii (Chlorophyceae, Chlorophyta) during batch culture development journal, November 2017

Mimicking lichens: incorporation of yeast strains together with sucrose-secreting cyanobacteria improves survival, growth, ROS removal, and lipid production in a stable mutualistic co-culture production platform journal, March 2017

Genetic engineering of fatty acid chain length in Phaeodactylum tricornutum
journal, January 2011

Biogenic hydrogen and methane production from Chlorella vulgaris and Dunaliella tertiolecta biomass
journal, January 2011

Genetic engineering of fatty acid chain length in Phaeodactylum tricornutum
journal, January 2011

Co-culturing a novel Bacillus strain with Clostridium tyrobutyricum ATCC 25755 to produce butyric acid from sucrose
journal, January 2013

Biofuels from algae: challenges and potential
journal, September 2010

Biodiesel production by simultaneous extraction and conversion of total lipids from microalgae, cyanobacteria, and wild mixed-cultures
journal, February 2011

Biofuels from algae: challenges and potential
journal, September 2010

Biogenic hydrogen and methane production from Chlorella vulgaris and Dunaliella tertiolecta biomass
journal, January 2011

Co-culturing a novel Bacillus strain with Clostridium tyrobutyricum ATCC 25755 to produce butyric acid from sucrose
journal, January 2013

Synechococcus sp. Strain PCC 7002 Transcriptome: Acclimation to Temperature, Salinity, Oxidative Stress, and Mixotrophic Growth Conditions
journal, January 2012

Determination of Unknown Concentrations of Sodium Acetate Using the Method of Standard Addition and Proton NMR: An Experiment for the Undergraduate Analytical Chemistry Laboratory
journal, August 2012

Increased Lipid Accumulation in the Chlamydomonas reinhardtii sta7-10 Starchless Isoamylase Mutant and Increased Carbohydrate Synthesis in Complemented Strains
journal, June 2010

Hydrogen production by sulfur-deprived Chlamydomonas reinhardtii under photoautotrophic conditions
journal, September 2006

Engineering algae for biohydrogen and biofuel production
journal, June 2009

Regulatory branch points affecting protein and lipid biosynthesis in the diatom Phaeodactylum tricornutum
journal, December 2013

Microalgae and terrestrial biomass as source for fuels—A process view
journal, June 2009

Biodiesel production by simultaneous extraction and conversion of total lipids from microalgae, cyanobacteria, and wild mixed-cultures
journal, February 2011

Demonstration of sustained hydrogen photoproduction by immobilized, sulfur-deprived Chlamydomonas reinhardtii cells
journal, April 2006

Altered carbohydrate metabolism in glycogen synthase mutants of Synechococcus sp. strain PCC 7002: Cell factories for soluble sugars
journal, March 2013

Second Generation Biofuels: High-Efficiency Microalgae for Biodiesel Production
journal, March 2008

Starchless Mutants of Chlamydomonas reinhardtii Lack the Small Subunit of a Heterotetrameric ADP-Glucose Pyrophosphorylase
journal, February 2001

Altered carbohydrate metabolism in glycogen synthase mutants of Synechococcus sp. strain PCC 7002: Cell factories for soluble sugars
journal, March 2013

Demonstration of sustained hydrogen photoproduction by immobilized, sulfur-deprived Chlamydomonas reinhardtii cells
journal, April 2006

Life cycle assessment of biodiesel production from microalgae in ponds
journal, January 2011

Hydrogen production by sulfur-deprived Chlamydomonas reinhardtii under photoautotrophic conditions
journal, September 2006

Phenotypic diversity of hydrogen production in chlorophycean algae reflects distinct anaerobic metabolisms
journal, June 2009

Redirecting Reductant Flux into Hydrogen Production via Metabolic Engineering of Fermentative Carbon Metabolism in a Cyanobacterium
journal, June 2010

NUTRITIONAL STUDIES WITH CHLAMYDOMONAS REINHARDI
journal, October 1953

Microalgae and terrestrial biomass as source for fuels—A process view
journal, June 2009

Determination of Unknown Concentrations of Sodium Acetate Using the Method of Standard Addition and Proton NMR: An Experiment for the Undergraduate Analytical Chemistry Laboratory
journal, August 2012

A quick isolation method for mutants with high lipid yield in oleaginous yeast
journal, January 2009

Engineering algae for biohydrogen and biofuel production
journal, June 2009

The Production of Hydrogen Peroxide by Blue-Green Algae: a Survey
journal, December 1973

Alignment of microbial fitness with engineered product formation: obligatory coupling between acetate production and photoautotrophic growth
journal, February 2018

Mimicking lichens: incorporation of yeast strains together with sucrose-secreting cyanobacteria improves survival, growth, ROS removal, and lipid production in a stable mutualistic co-culture production platform
journal, March 2017

Alignment of microbial fitness with engineered product formation: obligatory coupling between acetate production and photoautotrophic growth
journal, February 2018

Interactions Between Microalgae and Microorganisms for Wastewater Remediation and Biofuel Production
journal, May 2018

Lauric Acid Production in a Glycogen-Less Strain of Synechococcus sp. PCC 7002
journal, April 2015

Metabolomic and physiological changes of Chlamydomonas reinhardtii (Chlorophyceae, Chlorophyta) during batch culture development
journal, November 2017

Mimicking lichens: incorporation of yeast strains together with sucrose-secreting cyanobacteria improves survival, growth, ROS removal, and lipid production in a stable mutualistic co-culture production platform
journal, March 2017