Patterns in wetland microbial community composition and functional gene repertoire associated with methane emissions
Abstract
ABSTRACT Wetland restoration on peat islands previously drained for agriculture has potential to reverse land subsidence and sequester atmospheric carbon dioxide as peat accretes. However, the emission of methane could potentially offset the greenhouse gas benefits of captured carbon. As microbial communities play a key role in governing wetland greenhouse gas fluxes, we are interested in how microbial community composition and functions are associated with wetland hydrology, biogeochemistry, and methane emission, which is critical to modeling the microbial component in wetland methane fluxes and to managing restoration projects for maximal carbon sequestration. Here, we couple sequence-based methods with biogeochemical and greenhouse gas measurements to interrogate microbial communities from a pilot-scale restored wetland in the Sacramento-San Joaquin Delta of California, revealing considerable spatial heterogeneity even within this relatively small site. A number of microbial populations and functions showed strong correlations with electron acceptor availability and methane production; some also showed a preference for association with plant roots. Marker gene phylogenies revealed a diversity of major methane-producing and -consuming populations and suggested novel diversity within methanotrophs. Methanogenic archaea were observed in all samples, as were nitrate-, sulfate-, and metal-reducing bacteria, indicating that no single terminal electron acceptor was preferred despite differences inmore »
- Authors:
-
- DOE Joint Genome Institute, Walnut Creek, CA (United States)
- DOE Joint Genome Institute, Walnut Creek, CA (United States); Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
- U.S. Geological Survey, Menlo Park, CA (United States)
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1215649
- Alternate Identifier(s):
- OSTI ID: 1512194
- Grant/Contract Number:
- AC02-05CH11231
- Resource Type:
- Accepted Manuscript
- Journal Name:
- mBio (Online)
- Additional Journal Information:
- Journal Name: mBio (Online); Journal Volume: 6; Journal Issue: 3; Journal ID: ISSN 2150-7511
- Publisher:
- American Society for Microbiology
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 54 ENVIRONMENTAL SCIENCES
Citation Formats
He, Shaomei, Malfatti, Stephanie A., McFarland, Jack W., Anderson, Frank E., Pati, Amrita, Huntemann, Marcel, Tremblay, Julien, Glavina del Rio, Tijana, Waldrop, Mark P., Windham-Myers, Lisamarie, and Tringe, Susannah G. Patterns in wetland microbial community composition and functional gene repertoire associated with methane emissions. United States: N. p., 2015.
Web. doi:10.1128/mBio.00066-15.
He, Shaomei, Malfatti, Stephanie A., McFarland, Jack W., Anderson, Frank E., Pati, Amrita, Huntemann, Marcel, Tremblay, Julien, Glavina del Rio, Tijana, Waldrop, Mark P., Windham-Myers, Lisamarie, & Tringe, Susannah G. Patterns in wetland microbial community composition and functional gene repertoire associated with methane emissions. United States. https://doi.org/10.1128/mBio.00066-15
He, Shaomei, Malfatti, Stephanie A., McFarland, Jack W., Anderson, Frank E., Pati, Amrita, Huntemann, Marcel, Tremblay, Julien, Glavina del Rio, Tijana, Waldrop, Mark P., Windham-Myers, Lisamarie, and Tringe, Susannah G. Tue .
"Patterns in wetland microbial community composition and functional gene repertoire associated with methane emissions". United States. https://doi.org/10.1128/mBio.00066-15. https://www.osti.gov/servlets/purl/1215649.
@article{osti_1215649,
title = {Patterns in wetland microbial community composition and functional gene repertoire associated with methane emissions},
author = {He, Shaomei and Malfatti, Stephanie A. and McFarland, Jack W. and Anderson, Frank E. and Pati, Amrita and Huntemann, Marcel and Tremblay, Julien and Glavina del Rio, Tijana and Waldrop, Mark P. and Windham-Myers, Lisamarie and Tringe, Susannah G.},
abstractNote = {ABSTRACT Wetland restoration on peat islands previously drained for agriculture has potential to reverse land subsidence and sequester atmospheric carbon dioxide as peat accretes. However, the emission of methane could potentially offset the greenhouse gas benefits of captured carbon. As microbial communities play a key role in governing wetland greenhouse gas fluxes, we are interested in how microbial community composition and functions are associated with wetland hydrology, biogeochemistry, and methane emission, which is critical to modeling the microbial component in wetland methane fluxes and to managing restoration projects for maximal carbon sequestration. Here, we couple sequence-based methods with biogeochemical and greenhouse gas measurements to interrogate microbial communities from a pilot-scale restored wetland in the Sacramento-San Joaquin Delta of California, revealing considerable spatial heterogeneity even within this relatively small site. A number of microbial populations and functions showed strong correlations with electron acceptor availability and methane production; some also showed a preference for association with plant roots. Marker gene phylogenies revealed a diversity of major methane-producing and -consuming populations and suggested novel diversity within methanotrophs. Methanogenic archaea were observed in all samples, as were nitrate-, sulfate-, and metal-reducing bacteria, indicating that no single terminal electron acceptor was preferred despite differences in energetic favorability and suggesting spatial microheterogeneity and microniches. Notably, methanogens were negatively correlated with nitrate-, sulfate-, and metal-reducing bacteria and were most abundant at sampling sites with high peat accretion and low electron acceptor availability, where methane production was highest. IMPORTANCEWetlands are the largest nonanthropogenic source of atmospheric methane but also a key global carbon reservoir. Characterizing belowground microbial communities that mediate carbon cycling in wetlands is critical to accurately predicting their responses to changes in land management and climate. Here, we studied a restored wetland and revealed substantial spatial heterogeneity in biogeochemistry, methane production, and microbial communities, largely associated with the wetland hydraulic design. We observed patterns in microbial community composition and functions correlated with biogeochemistry and methane production, including diverse microorganisms involved in methane production and consumption. We found that methanogenesis gene abundance is inversely correlated with genes from pathways exploiting other electron acceptors, yet the ubiquitous presence of genes from all these pathways suggests that diverse electron acceptors contribute to the energetic balance of the ecosystem. These investigations represent an important step toward effective management of wetlands to reduce methane flux to the atmosphere and enhance belowground carbon storage.},
doi = {10.1128/mBio.00066-15},
journal = {mBio (Online)},
number = 3,
volume = 6,
place = {United States},
year = {Tue May 19 00:00:00 EDT 2015},
month = {Tue May 19 00:00:00 EDT 2015}
}
Web of Science
Works referenced in this record:
A novel family of functional operons encoding methane/ammonia monooxygenase-related proteins in gammaproteobacterial methanotrophs: Novel monooxygenase in Gamma-MOB
journal, February 2011
- Tavormina, Patricia L.; Orphan, Victoria J.; Kalyuzhnaya, Marina G.
- Environmental Microbiology Reports, Vol. 3, Issue 1
Plant community, primary productivity, and environmental conditions following wetland re-establishment in the Sacramento-San Joaquin Delta, California
journal, May 2009
- Miller, Robin L.; Fujii, Roger
- Wetlands Ecology and Management, Vol. 18, Issue 1
IMG/M 4 version of the integrated metagenome comparative analysis system
journal, October 2013
- Markowitz, Victor M.; Chen, I-Min A.; Chu, Ken
- Nucleic Acids Research, Vol. 42, Issue D1, p. D568-D573
Improved coring device for measuring soil bulk density in a Louisiana deltaic marsh
journal, July 1994
- Hargis, T. G.; Twilley, R. R.
- Journal of Sedimentary Research, Vol. 64, Issue 3a
Diversity and Community Structure of Archaea Inhabiting the Rhizoplane of Two Contrasting Plants from an Acidic Bog
journal, December 2009
- Cadillo-Quiroz, Hinsby; Yavitt, Joseph B.; Zinder, Stephen H.
- Microbial Ecology, Vol. 59, Issue 4
New Algorithms and Methods to Estimate Maximum-Likelihood Phylogenies: Assessing the Performance of PhyML 3.0
journal, March 2010
- Guindon, Stéphane; Dufayard, Jean-François; Lefort, Vincent
- Systematic Biology, Vol. 59, Issue 3
Comparative Metagenomics of Microbial Communities
journal, April 2005
- Tringe, S. G.
- Science, Vol. 308, Issue 5721
Fast and accurate short read alignment with Burrows-Wheeler transform
journal, May 2009
- Li, H.; Durbin, R.
- Bioinformatics, Vol. 25, Issue 14
An Improved General Amino Acid Replacement Matrix
journal, April 2008
- Le, S. Q.; Gascuel, O.
- Molecular Biology and Evolution, Vol. 25, Issue 7
Effects of nitrate, nitrite, NO and N2O on methanogenesis and other redox processes in anoxic rice field soil
journal, March 1998
- Klüber, H.
- FEMS Microbiology Ecology, Vol. 25, Issue 3
Oxygen Sensitivity of Methanogenic Bacteria
journal, August 1983
- Kiener, Andreas; Leisinger, Thomas
- Systematic and Applied Microbiology, Vol. 4, Issue 3
Methanotrophic bacteria.
journal, January 1996
- Hanson, R. S.; Hanson, T. E.
- Microbiological reviews, Vol. 60, Issue 2
Subsidence Reversal in a Re-Established Wetland in the Sacramento-San Joaquin Delta, California, USA [Subsidence Reversal in a Re-Established Wetland in the Sacramento-San Joaquin Delta, California, USA]
journal, October 2008
- Miller, Robin; Fram, Miranda S.; Fujii, Roger
- San Francisco Estuary and Watershed Science, Vol. 6, Issue 3
Land-cover change in the conterminous United States from 1973 to 2000
journal, August 2013
- Sleeter, Benjamin M.; Sohl, Terry L.; Loveland, Thomas R.
- Global Environmental Change, Vol. 23, Issue 4
Improved Assemblies Using a Source-Agnostic Pipeline for MetaGenomic Assembly by Merging (MeGAMerge) of Contigs
journal, October 2014
- Scholz, Matthew; Lo, Chien-Chi; Chain, Patrick S. G.
- Scientific Reports, Vol. 4, Issue 1
Wetlands, carbon, and climate change
journal, June 2012
- Mitsch, William J.; Bernal, Blanca; Nahlik, Amanda M.
- Landscape Ecology, Vol. 28, Issue 4
Hydrologic and Water-Quality Conditions During Restoration of the Wood River Wetland, Upper Klamath River Basin, Oregon, 2003-05
report, January 2009
- Carpenter, Kurt D.; Snyder, Daniel T.; Duff, John H.
Sulfate Reducers Can Outcompete Methanogens at Freshwater Sulfate Concentrations †
journal, January 1983
- Lovley, Derek R.; Klug, Michael J.
- Applied and Environmental Microbiology, Vol. 45, Issue 1
Carbon Storage and Fluxes within Freshwater Wetlands: a Critical Review
journal, December 2009
- Kayranli, Birol; Scholz, Miklas; Mustafa, Atif
- Wetlands, Vol. 30, Issue 1
Competition between Fe(III)-Reducing and Methanogenic Bacteria for Acetate in Iron-Rich Freshwater Sediments
journal, March 2003
- Roden, E. E.; Wetzel, R. G.
- Microbial Ecology, Vol. 45, Issue 3
New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0
text, January 2010
- Guindon, Stéphane; Dufayard, Jean-François; Lefort, Vincent
- Oxford University Press
Seasonal and spatial patterns of oxygen production, respiration and root-rhizome release in Potamogeton perfoliatus L. and Zostera marina L.
journal, January 1991
- Caffrey, J. M.; Kemp, W. M.
- Aquatic Botany, Vol. 40, Issue 2
Methanoregula boonei gen. nov., sp. nov., an acidiphilic methanogen isolated from an acidic peat bog
journal, February 2010
- Brauer, S. L.; Cadillo-Quiroz, H.; Ward, R. J.
- INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 61, Issue 1
Methanoregula formicica sp. nov., a methane-producing archaeon isolated from methanogenic sludge
journal, August 2009
- Yashiro, Y.; Sakai, S.; Ehara, M.
- INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 61, Issue 1
Subsidence of Agricultural Lands in the Sacramento-San Joaquin Delta, California: Role of Aqueous and Gaseous Carbon Fluxes
journal, August 1996
- Deverel, Steven J.; Rojstaczer, Stuart
- Water Resources Research, Vol. 32, Issue 8
Methane emission from natural wetlands: interplay between emergent macrophytes and soil microbial processes. A mini-review
journal, August 2009
- Laanbroek, Hendrikus J.
- Annals of Botany, Vol. 105, Issue 1
Why do sulfate-reducing bacteria outcompete methanogenic bacteria for substrates?
journal, November 1983
- Kristjansson, J. K.; Sch�nheit, P.
- Oecologia, Vol. 60, Issue 2
A new type of thiosulphate oxidizing, nitrate reducing microorganism: Thiomicrospira denitrificans sp. Nov.
journal, December 1975
- Hoor, Anje Timmer-Ten
- Netherlands Journal of Sea Research, Vol. 9, Issue 3-4
Root Exudation and Rhizosphere Biology: Fig. 1.
journal, May 2003
- Walker, Travis S.; Bais, Harsh Pal; Grotewold, Erich
- Plant Physiology, Vol. 132, Issue 1
Experimental factors affecting PCR-based estimates of microbial species richness and evenness
journal, January 2010
- Engelbrektson, Anna; Kunin, Victor; Wrighton, Kelly C.
- The ISME Journal, Vol. 4, Issue 5
Greenhouse carbon balance of wetlands: methane emission versus carbon sequestration
journal, January 2001
- Whiting, Gary J.; Chanton, Jeffrey P.
- Tellus B: Chemical and Physical Meteorology, Vol. 53, Issue 5
Methane oxidation by an extremely acidophilic bacterium of the phylum Verrucomicrobia
journal, November 2007
- Dunfield, Peter F.; Yuryev, Anton; Senin, Pavel
- Nature, Vol. 450, Issue 7171
Shewanella putrefaciens mtrB Encodes an Outer Membrane Protein Required for Fe(III) and Mn(IV) Reduction
journal, December 1998
- Beliaev, Alexander S.; Saffarini, Daad A.
- Journal of Bacteriology, Vol. 180, Issue 23
The effect of vascular plants on carbon turnover and methane emissions from a tundra wetland
journal, August 2003
- Strom, Lena; Ekberg, Anna; Mastepanov, Mikhail
- Global Change Biology, Vol. 9, Issue 8
Wrinkles in the rare biosphere: pyrosequencing errors can lead to artificial inflation of diversity estimates
journal, January 2010
- Kunin, Victor; Engelbrektson, Anna; Ochman, Howard
- Environmental Microbiology, Vol. 12, Issue 1
Identification and Characterization of MtoA: A Decaheme c-Type Cytochrome of the Neutrophilic Fe(II)-Oxidizing Bacterium Sideroxydans lithotrophicus ES-1
journal, January 2012
- Liu, Juan; Wang, Zheming; Belchik, Sara M.
- Frontiers in Microbiology, Vol. 3
Revised Taxonomy of the Methanotrophs: Description of Methylobacter gen. nov., Emendation of Methylococcus, Validation of Methylosinus and Methylocystis Species, and a Proposal that the Family Methylococcaceae Includes Only the Group I Methanotrophs
journal, October 1993
- Bowman, J. P.; Sly, L. I.; Nichols, P. D.
- International Journal of Systematic Bacteriology, Vol. 43, Issue 4
The role of root Exudates in Rhizosphere Interactions with Plants and Other Organisms
journal, June 2006
- Bais, Harsh P.; Weir, Tiffany L.; Perry, Laura G.
- Annual Review of Plant Biology, Vol. 57, Issue 1
Wide diversity of Crenarchaeota
journal, December 1996
- Hershberger, Karen L.; Barns, Susan M.; Reysenbach, Anna-Louise
- Nature, Vol. 384, Issue 6608
Methane emission from rice: Stable isotopes, diurnal variations, and CO 2 exchange
journal, March 1997
- Chanton, J. P.; Whiting, G. J.; Blair, N. E.
- Global Biogeochemical Cycles, Vol. 11, Issue 1
Methanogenesis from acetate: a comparison of the acetate metabolism in Methanothrix soehngenii and Methanosarcina spp.
journal, June 1992
- Jetten, M.
- FEMS Microbiology Letters, Vol. 88, Issue 3-4
The structure and continuity of the lacunar system of the seagrass Halophila ovalis (R. Br.) Hook f. (Hydrocharitaceae)
journal, June 1984
- Roberts, D. G.; McComb, A. J.; Kuo, J.
- Aquatic Botany, Vol. 18, Issue 4
Metabolic, Phylogenetic, and Ecological Diversity of the Methanogenic Archaea
journal, March 2008
- Liu, Yuchen; Whitman, William B.
- Annals of the New York Academy of Sciences, Vol. 1125, Issue 1
Novel Euryarchaeotal Lineages Detected on Rice Roots and in the Anoxic Bulk Soil of Flooded Rice Microcosms
journal, December 1998
- Großkopf, Regine; Stubner, Stephan; Liesack, Werner
- Applied and Environmental Microbiology, Vol. 64, Issue 12
A sand fly salivary protein acts as a neutrophil chemoattractant
journal, May 2021
- Guimaraes-Costa, Anderson B.; Shannon, John P.; Waclawiak, Ingrid
- Nature Communications, Vol. 12, Issue 1
Revised Taxonomy of the Methanotrophs: Description of Methylobacter gen. nov., Emendation of Methylococcus, Validation of Methylosinus and Methylocystis Species, and a Proposal that the Family Methylococcaceae Includes Only the Group I Methanotrophs
journal, April 1994
- Bowman, J. P.; Sly, L. I.; Nichols, P. D.
- International Journal of Systematic Bacteriology, Vol. 44, Issue 2
Metagenomic 16S rDNA Illumina tags are a powerful alternative to amplicon sequencing to explore diversity and structure of microbial communities: Using
journal, September 2013
- Logares, Ramiro; Sunagawa, Shinichi; Salazar, Guillem
- Environmental Microbiology, Vol. 16, Issue 9
Nitrite-driven anaerobic methane oxidation by oxygenic bacteria
journal, March 2010
- Ettwig, Katharina F.; Butler, Margaret K.; Le Paslier, Denis
- Nature, Vol. 464, Issue 7288
Activation of Methanogenesis in Arid Biological Soil Crusts Despite the Presence of Oxygen
journal, May 2011
- Angel, Roey; Matthies, Diethart; Conrad, Ralf
- PLoS ONE, Vol. 6, Issue 5
Respiration of metal (hydr)oxides by Shewanella and Geobacter : a key role for multihaem c -type cytochromes
journal, July 2007
- Shi, Liang; Squier, Thomas C.; Zachara, John M.
- Molecular Microbiology, Vol. 65, Issue 1
The Genome Sequence of the Obligately Chemolithoautotrophic, Facultatively Anaerobic Bacterium Thiobacillus denitrificans
journal, February 2006
- Beller, H. R.; Chain, P. S. G.; Letain, T. E.
- Journal of Bacteriology, Vol. 188, Issue 4
Greenhouse carbon balance of wetlands: methane emission versus carbon sequestration: Greenhouse carbon balance of wetlands
journal, November 2001
- Whiting, Gary J.; Chanton, Jeffrey P.
- Tellus B, Vol. 53, Issue 5
MUSCLE: a multiple sequence alignment method with reduced time and space complexity
journal, August 2004
- Edgar, Robert C.
- BMC Bioinformatics, Vol. 5, Issue 1, p. 113
Methanogenic archaea: ecologically relevant differences in energy conservation
journal, June 2008
- Thauer, Rudolf K.; Kaster, Anne-Kristin; Seedorf, Henning
- Nature Reviews Microbiology, Vol. 6, Issue 8
Methane emissions from wetlands: biogeochemical, microbial, and modeling perspectives from local to global scales
journal, February 2013
- Bridgham, Scott D.; Cadillo-Quiroz, Hinsby; Keller, Jason K.
- Global Change Biology, Vol. 19, Issue 5
Processes involved in formation and emission of methane in rice paddies
journal, January 1989
- Sch�tz, Helmut; Seiler, Wolfgang; Conrad, Ralf
- Biogeochemistry, Vol. 7, Issue 1
New Algorithms and Methods to Estimate Maximum-Likelihood Phylogenies: Assessing the Performance of PhyML 3.0
text, January 2010
- Guindon, Stéphane; Dufayard, Jean-François; Lefort, Vincent
- ETH Zurich
Towards the definition of a core of microorganisms involved in anaerobic digestion of sludge
journal, February 2009
- Rivière, Delphine; Desvignes, Virginie; Pelletier, Eric
- The ISME Journal, Vol. 3, Issue 6
Regulation of methane oxidation in the facultative methanotroph Methylocella silvestris BL2 : Methane oxidation in Methylocella
journal, September 2005
- Theisen, Andreas R.; Ali, M. Hanif; Radajewski, Stefan
- Molecular Microbiology, Vol. 58, Issue 3
Inhibitory effects of nitrate, nitrite, NO and N2O on methanogenesis by Methanosarcina barkeri and Methanobacterium bryantii
journal, April 1998
- Klüber, H.
- FEMS Microbiology Ecology, Vol. 25, Issue 4
Molecular Ecology Techniques for the Study of Aerobic Methanotrophs
journal, December 2007
- McDonald, I. R.; Bodrossy, L.; Chen, Y.
- Applied and Environmental Microbiology, Vol. 74, Issue 5
Two isozymes of particulate methane monooxygenase with different methane oxidation kinetics are found in Methylocystis sp. strain SC2
journal, July 2008
- Baani, M.; Liesack, W.
- Proceedings of the National Academy of Sciences, Vol. 105, Issue 29
Biochemical cycling in the rhizosphere having an impact on global change
journal, October 2008
- Philippot, L.; Hallin, S.; Börjesson, G.
- Plant and Soil, Vol. 321, Issue 1-2
Carbon Gas Fluxes in Re-Established Wetlands on Organic Soils Differ Relative to Plant Community and Hydrology
journal, August 2011
- Miller, Robin L.
- Wetlands, Vol. 31, Issue 6
Production, oxidation, emission and consumption of methane by soils: A review
journal, January 2001
- Le Mer, Jean; Roger, Pierre
- European Journal of Soil Biology, Vol. 37, Issue 1
Yet-to-be Cultured Microorganisms Relevant to Methane Fermentation Processes
journal, January 2006
- Sekiguchi, Yuji
- Microbes and Environments, Vol. 21, Issue 1
The legacy of wetland drainage on the remaining peat in the Sacramento — San Joaquin Delta, California, USA
journal, March 2009
- Drexler, Judith Z.; Fontaine, Christian S.; Deverel, Steven J.
- Wetlands, Vol. 29, Issue 1
Phylogeny of sulfate-reducing bacteria1
journal, January 2000
- Castro, Hector F.; Williams, Norris H.; Ogram, Andrew
- FEMS Microbiology Ecology, Vol. 31, Issue 1
Works referencing / citing this record:
Patterns of Denitrification and Methanogenesis Rates from Vernal Pools in a Temperate Forest Driven by Seasonal, Microbial Functional Gene Abundances, and Soil Chemistry
journal, January 2020
- Ayayee, Paul A.; Taura, Joseph; Roberto, Alescia A.
- Wetlands, Vol. 40, Issue 4
Potential for negative emissions of greenhouse gases (CO 2 , CH 4 and N 2 O) through coastal peatland re-establishment: Novel insights from high frequency flux data at meter and kilometer scales
journal, March 2018
- Windham-Myers, Lisamarie; Bergamaschi, Brian; Anderson, Frank
- Environmental Research Letters, Vol. 13, Issue 4
Primer and platform effects on 16S rRNA tag sequencing
journal, August 2015
- Tremblay, Julien; Singh, Kanwar; Fern, Alison
- Frontiers in Microbiology, Vol. 6
Predominance of methanogens over methanotrophs in rewetted fens characterized by high methane emissions
journal, January 2018
- Wen, Xi; Unger, Viktoria; Jurasinski, Gerald
- Biogeosciences, Vol. 15, Issue 21
Ecological selection for small microbial genomes along a temperate-to-thermal soil gradient
journal, November 2018
- Sorensen, Jackson W.; Dunivin, Taylor K.; Tobin, Tammy C.
- Nature Microbiology, Vol. 4, Issue 1
The role of wetland microorganisms in plant-litter decomposition and soil organic matter formation: a critical review
journal, August 2018
- Yarwood, Stephanie A.
- FEMS Microbiology Ecology, Vol. 94, Issue 11
Phylogenetic and genomic analysis of Methanomassiliicoccales in wetlands and animal intestinal tracts reveals clade-specific habitat preferences
journal, November 2015
- Söllinger, Andrea; Schwab, Clarissa; Weinmaier, Thomas
- FEMS Microbiology Ecology, Vol. 92, Issue 1
Abundant carbon substrates drive extremely high sulfate reduction rates and methane fluxes in Prairie Pothole Wetlands
journal, February 2017
- Dalcin Martins, Paula; Hoyt, David W.; Bansal, Sheel
- Global Change Biology, Vol. 23, Issue 8
High-resolution sequencing reveals unexplored archaeal diversity in freshwater wetland soils: Freshwater wetland archaeal diversity
journal, March 2017
- Narrowe, Adrienne B.; Angle, Jordan C.; Daly, Rebecca A.
- Environmental Microbiology, Vol. 19, Issue 6
Soil Carbon-Fixation Rates and Associated Bacterial Diversity and Abundance in Three Natural Ecosystems
journal, November 2016
- Lynn, Tin Mar; Ge, Tida; Yuan, Hongzhao
- Microbial Ecology, Vol. 73, Issue 3
Typha (Cattail) Invasion in North American Wetlands: Biology, Regional Problems, Impacts, Ecosystem Services, and Management
journal, June 2019
- Bansal, Sheel; Lishawa, Shane C.; Newman, Sue
- Wetlands, Vol. 39, Issue 4
NCycDB: a curated integrative database for fast and accurate metagenomic profiling of nitrogen cycling genes
journal, August 2018
- Tu, Qichao; Lin, Lu; Cheng, Lei
- Bioinformatics, Vol. 35, Issue 6
Where old meets new: An ecosystem study of methanogenesis in a reflooded agricultural peatland
journal, December 2019
- McNicol, Gavin; Knox, Sara H.; Guilderson, Thomas P.
- Global Change Biology, Vol. 26, Issue 2
Primer and platform effects on 16S rRNA tag sequencing
text, January 2015
- Alison, Fern,; Feng, Chen,; Julien, Tremblay,
- The University of North Carolina at Chapel Hill University Libraries