The pilus usher controls protein interactions via domain masking and is functional as an oligomer
Abstract
The chaperone/usher (CU) pathway is responsible for biogenesis of organelles termed pili or fimbriae in Gram-negative bacteria. Type 1 pili expressed by uropathogenic Escherichia coli are prototypical structures assembled by the CU pathway. Assembly and secretion of pili by the CU pathway requires a dedicated periplasmic chaperone and a multidomain outer membrane protein termed the usher (FimD). We show that the FimD C-terminal domains provide the high-affinity substrate binding site, but that these domains are masked in the resting usher. Domain masking requires the FimD plug domain, which served as a central switch controlling usher activation. In addition, we demonstrate that usher molecules can act in trans for pilus biogenesis, providing conclusive evidence for a functional usher oligomer. These results reveal mechanisms by which molecular machines such as the usher regulate and harness protein-protein interactions, and suggest that ushers may interact in a cooperative manner during pilus assembly in bacteria.
- Authors:
-
- Stony Brook Univ., Stony Brook, NY (United States)
- Stony Brook Univ., Stony Brook, NY (United States); Brookhaven National Lab. (BNL), Upton, NY (United States)
- Washington Univ., St. Louis, MO (United States)
- Publication Date:
- Research Org.:
- Brookhaven National Laboratory (BNL), Upton, NY (United States)
- Sponsoring Org.:
- National Institute of Health
- OSTI Identifier:
- 1215608
- Report Number(s):
- BNL-108346-2015-JA
Journal ID: ISSN 1545-9993; 600301010
- Grant/Contract Number:
- SC00112704
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Nature Structural & Molecular Biology
- Additional Journal Information:
- Journal Volume: 22; Journal Issue: 7; Journal ID: ISSN 1545-9993
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; membrane proteins; pathogens; protein translocation
Citation Formats
Werneburg, Glenn T., Li, Huilin, Henderson, Nadine S., Portnoy, Erica B., Sarowar, Samema, Hultgren, Scott J., and Thanassi, David G. The pilus usher controls protein interactions via domain masking and is functional as an oligomer. United States: N. p., 2015.
Web. doi:10.1038/nsmb.3044.
Werneburg, Glenn T., Li, Huilin, Henderson, Nadine S., Portnoy, Erica B., Sarowar, Samema, Hultgren, Scott J., & Thanassi, David G. The pilus usher controls protein interactions via domain masking and is functional as an oligomer. United States. https://doi.org/10.1038/nsmb.3044
Werneburg, Glenn T., Li, Huilin, Henderson, Nadine S., Portnoy, Erica B., Sarowar, Samema, Hultgren, Scott J., and Thanassi, David G. Mon .
"The pilus usher controls protein interactions via domain masking and is functional as an oligomer". United States. https://doi.org/10.1038/nsmb.3044. https://www.osti.gov/servlets/purl/1215608.
@article{osti_1215608,
title = {The pilus usher controls protein interactions via domain masking and is functional as an oligomer},
author = {Werneburg, Glenn T. and Li, Huilin and Henderson, Nadine S. and Portnoy, Erica B. and Sarowar, Samema and Hultgren, Scott J. and Thanassi, David G.},
abstractNote = {The chaperone/usher (CU) pathway is responsible for biogenesis of organelles termed pili or fimbriae in Gram-negative bacteria. Type 1 pili expressed by uropathogenic Escherichia coli are prototypical structures assembled by the CU pathway. Assembly and secretion of pili by the CU pathway requires a dedicated periplasmic chaperone and a multidomain outer membrane protein termed the usher (FimD). We show that the FimD C-terminal domains provide the high-affinity substrate binding site, but that these domains are masked in the resting usher. Domain masking requires the FimD plug domain, which served as a central switch controlling usher activation. In addition, we demonstrate that usher molecules can act in trans for pilus biogenesis, providing conclusive evidence for a functional usher oligomer. These results reveal mechanisms by which molecular machines such as the usher regulate and harness protein-protein interactions, and suggest that ushers may interact in a cooperative manner during pilus assembly in bacteria.},
doi = {10.1038/nsmb.3044},
journal = {Nature Structural & Molecular Biology},
number = 7,
volume = 22,
place = {United States},
year = {Mon Jun 08 00:00:00 EDT 2015},
month = {Mon Jun 08 00:00:00 EDT 2015}
}
Web of Science
Works referenced in this record:
The Role of Chaperone-subunit Usher Domain Interactions in the Mechanism of Bacterial Pilus Biogenesis Revealed by ESI-MS
journal, February 2012
- Morrissey, Bethny; Leney, Aneika C.; Toste Rêgo, Ana
- Molecular & Cellular Proteomics, Vol. 11, Issue 7
The Gal(alpha 1-4)Gal-specific tip adhesin of Escherichia coli P-fimbriae is needed for pyelonephritis to occur in the normal urinary tract.
journal, December 1994
- Roberts, J. A.; Marklund, B. I.; Ilver, D.
- Proceedings of the National Academy of Sciences, Vol. 91, Issue 25
FimH adhesin of type 1 pili is assembled into a fibrillar tip structure in the Enterobacteriaceae.
journal, March 1995
- Jones, C. H.; Pinkner, J. S.; Roth, R.
- Proceedings of the National Academy of Sciences, Vol. 92, Issue 6
Function of the usher N-terminus in catalysing pilus assembly: Catalytic activity of the usher
journal, December 2010
- Henderson, Nadine S.; Ng, Tony W.; Talukder, Iehab
- Molecular Microbiology, Vol. 79, Issue 4
Structural basis of chaperone–subunit complex recognition by the type 1 pilus assembly platform FimD
journal, May 2005
- Nishiyama, Mireille; Horst, Reto; Eidam, Oliv
- The EMBO Journal, Vol. 24, Issue 12
InsPecT: Identification of Posttranslationally Modified Peptides from Tandem Mass Spectra
journal, July 2005
- Tanner, Stephen; Shu, Hongjun; Frank, Ari
- Analytical Chemistry, Vol. 77, Issue 14
Site-directed, Ligase-Independent Mutagenesis (SLIM): a single-tube methodology approaching 100% efficiency in 4 h
journal, November 2004
- Chiu, J.
- Nucleic Acids Research, Vol. 32, Issue 21
Periplasmic Chaperones—Preservers of Subunit Folding Energy for Organelle Assembly
journal, May 2003
- Behrens, Susanne
- Cell, Vol. 113, Issue 5
Insights into pilus assembly and secretion from the structure and functional characterization of usher PapC
journal, April 2009
- Huang, Y.; Smith, B. S.; Chen, L. X.
- Proceedings of the National Academy of Sciences, Vol. 106, Issue 18
Structural and energetic basis of folded-protein transport by the FimD usher
journal, April 2013
- Geibel, Sebastian; Procko, Erik; Hultgren, Scott J.
- Nature, Vol. 496, Issue 7444
The Gal(alpha 1-4)Gal-specific tip adhesin of Escherichia coli P-fimbriae is needed for pyelonephritis to occur in the normal urinary tract.
journal, December 1994
- Roberts, J. A.; Marklund, B. I.; Ilver, D.
- Proceedings of the National Academy of Sciences, Vol. 91, Issue 25
Ramifications of kinetic partitioning on usher-mediated pilus biogenesis
journal, April 1998
- Saulino, E. T.
- The EMBO Journal, Vol. 17, Issue 8
Two copies of the SecY channel and acidic lipids are necessary to activate the SecA translocation ATPase
journal, February 2012
- Dalal, K.; Chan, C. S.; Sligar, S. G.
- Proceedings of the National Academy of Sciences, Vol. 109, Issue 11
Reconstitution of Pilus Assembly Reveals a Bacterial Outer Membrane Catalyst
journal, April 2008
- Nishiyama, M.; Ishikawa, T.; Rechsteiner, H.
- Science, Vol. 320, Issue 5874
FimH adhesin of type 1 pili is assembled into a fibrillar tip structure in the Enterobacteriaceae.
journal, March 1995
- Jones, C. H.; Pinkner, J. S.; Roth, R.
- Proceedings of the National Academy of Sciences, Vol. 92, Issue 6
Stoichiometry of SecYEG in the active translocase of Escherichia coli varies with precursor species
journal, July 2013
- Mao, C.; Cheadle, C. E.; Hardy, S. J. S.
- Proceedings of the National Academy of Sciences, Vol. 110, Issue 29
Crystal structure of the FimD usher bound to its cognate FimC–FimH substrate
journal, June 2011
- Phan, Gilles; Remaut, Han; Wang, Tao
- Nature, Vol. 474, Issue 7349
The molecular dissection of the chaperone–usher pathway
journal, August 2014
- Geibel, Sebastian; Waksman, Gabriel
- Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Vol. 1843, Issue 8
Structural and energetic basis of folded-protein transport by the FimD usher
journal, April 2013
- Geibel, Sebastian; Procko, Erik; Hultgren, Scott J.
- Nature, Vol. 496, Issue 7444
Exploring the 3D Molecular Architecture of Escherichia coli Type 1 Pili
journal, November 2002
- Hahn, Erik; Wild, Peter; Hermanns, Uta
- Journal of Molecular Biology, Vol. 323, Issue 5
Structural basis of chaperone–subunit complex recognition by the type 1 pilus assembly platform FimD
journal, May 2005
- Nishiyama, Mireille; Horst, Reto; Eidam, Oliv
- The EMBO Journal, Vol. 24, Issue 12
Site-directed, Ligase-Independent Mutagenesis (SLIM): a single-tube methodology approaching 100% efficiency in 4 h
journal, November 2004
- Chiu, J.
- Nucleic Acids Research, Vol. 32, Issue 21
How Chaperones Protect Virgin Proteins
journal, August 1999
- Eisenberg, David
- Science, Vol. 285, Issue 5430
Modulating Effects of the Plug, Helix, and N- and C-terminal Domains on Channel Properties of the PapC Usher
journal, October 2009
- Mapingire, Owen S.; Henderson, Nadine S.; Duret, Guillaume
- Journal of Biological Chemistry, Vol. 284, Issue 52
The Outer Membrane Usher Forms a Twin-pore Secretion Complex
journal, December 2004
- Li, Huilin; Qian, Luping; Chen, Zhiqiang
- Journal of Molecular Biology, Vol. 344, Issue 5
Unraveling the molecular basis of subunit specificity in P pilus assembly by mass spectrometry
journal, August 2008
- Rose, R. J.; Verger, D.; Daviter, T.
- Proceedings of the National Academy of Sciences, Vol. 105, Issue 35
Addition of a photocrosslinking amino acid to the genetic code of Escherichia coli
journal, August 2002
- Chin, J. W.; Martin, A. B.; King, D. S.
- Proceedings of the National Academy of Sciences, Vol. 99, Issue 17, p. 11020-11024
Structure and Biogenesis of the Capsular F1 Antigen from Yersinia pestis
journal, May 2003
- Zavialov, Anton V.; Berglund, Jenny; Pudney, Alexander F.
- Cell, Vol. 113, Issue 5
Surface organelles assembled by secretion systems of Gram-negative bacteria: diversity in structure and function
journal, November 2012
- Thanassi, David G.; Bliska, James B.; Christie, Peter J.
- FEMS Microbiology Reviews, Vol. 36, Issue 6
The differential affinity of the usher for chaperone-subunit complexes is required for assembly of complete pili
journal, April 2010
- Li, Qinyuan; Ng, Tony W.; Dodson, Karen W.
- Molecular Microbiology, Vol. 76, Issue 1
The differential affinity of the usher for chaperone-subunit complexes is required for assembly of complete pili
journal, April 2010
- Li, Qinyuan; Ng, Tony W.; Dodson, Karen W.
- Molecular Microbiology, Vol. 76, Issue 1
Chaperone Priming of Pilus Subunits Facilitates a Topological Transition that Drives Fiber Formation
journal, November 2002
- Sauer, Frederic G.; Pinkner, Jerome S.; Waksman, Gabriel
- Cell, Vol. 111, Issue 4
The Outer Membrane Usher Guarantees the Formation of Functional Pili by Selectively Catalyzing Donor-Strand Exchange between Subunits That Are Adjacent in the Mature Pilus
journal, February 2010
- Nishiyama, Mireille; Glockshuber, Rudi
- Journal of Molecular Biology, Vol. 396, Issue 1
Protein Insertion into the Mitochondrial Inner Membrane by a Twin-Pore Translocase
journal, March 2003
- Rehling, P.
- Science, Vol. 299, Issue 5613
Conserved Hydrophobic Clusters on the Surface of the Caf1A Usher C-Terminal Domain Are Important for F1 Antigen Assembly
journal, October 2010
- Dubnovitsky, Anatoly P.; Duck, Zoe; Kersley, Joanne E.
- Journal of Molecular Biology, Vol. 403, Issue 2
Twin Ushers Guide Pili across the Bacterial Outer Membrane
journal, May 2008
- Daniels, Robert; Normark, Staffan
- Cell, Vol. 133, Issue 4
Mass Spectrometric Sequencing of Proteins from Silver-Stained Polyacrylamide Gels
journal, January 1996
- Shevchenko, Andrej; Wilm, Matthias; Vorm, Ole
- Analytical Chemistry, Vol. 68, Issue 5
Caf1A usher possesses a Caf1 subunit-like domain that is crucial for Caf1 fibre secretion
journal, February 2009
- Yu, Xiaodi; Visweswaran, Ganeshram R.; Duck, Zoe
- Biochemical Journal, Vol. 418, Issue 3
Dissection of Pilus Tip Assembly by the FimD Usher Monomer
journal, March 2013
- Allen, William J.; Phan, Gilles; Hultgren, Scott J.
- Journal of Molecular Biology, Vol. 425, Issue 5
Tom40, the Pore-Forming Component of the Protein-Conducting Tom Channel in the Outer Membrane of Mitochondria
journal, June 2001
- Ahting, Uwe; Thieffry, Michel; Engelhardt, Harald
- Journal of Cell Biology, Vol. 153, Issue 6
Site-directed, Ligase-Independent Mutagenesis (SLIM) for highly efficient mutagenesis of plasmids greater than 8kb
journal, May 2008
- Chiu, Joyce; Tillett, Daniel; Dawes, Ian W.
- Journal of Microbiological Methods, Vol. 73, Issue 2
Protein secretion in the absence of ATP: the autotransporter, two-partner secretion and chaperone/usher pathways of Gram-negative bacteria (Review)
journal, January 2005
- Thanassi, David G.; Stathopoulos, Christos; Karkal, Aarthi
- Molecular Membrane Biology, Vol. 22, Issue 1-2
The Oligomeric State and Arrangement of the Active Bacterial Translocon
journal, November 2010
- Deville, Karine; Gold, Vicki A. M.; Robson, Alice
- Journal of Biological Chemistry, Vol. 286, Issue 6
Domain activities of PapC usher reveal the mechanism of action of an Escherichia coli molecular machine
journal, May 2012
- Volkan, E.; Ford, B. A.; Pinkner, J. S.
- Proceedings of the National Academy of Sciences, Vol. 109, Issue 24
An Enhanced System for Unnatural Amino Acid Mutagenesis in E. coli
journal, January 2010
- Young, Travis S.; Ahmad, Insha; Yin, Jun A.
- Journal of Molecular Biology, Vol. 395, Issue 2
Fiber Formation across the Bacterial Outer Membrane by the Chaperone/Usher Pathway
journal, May 2008
- Remaut, Han; Tang, Chunyan; Henderson, Nadine S.
- Cell, Vol. 133, Issue 4
Mass Spectrometric Sequencing of Proteins from Silver-Stained Polyacrylamide Gels
journal, January 1996
- Shevchenko, Andrej; Wilm, Matthias; Vorm, Ole
- Analytical Chemistry, Vol. 68, Issue 5
Adhesive organelles of Gram-negative pathogens assembled with the classical chaperone/usher machinery: structure and function from a clinical standpoint
journal, May 2010
- Zav'yalov, Vladimir; Zavialov, Anton; Zav'yalova, Galina
- FEMS Microbiology Reviews, Vol. 34, Issue 3
Induction and Evasion of Host Defenses by Type 1-Piliated Uropathogenic Escherichia coli
journal, November 1998
- Mulvey, M. A.
- Science, Vol. 282, Issue 5393
The PapC usher forms an oligomeric channel: Implications for pilus biogenesis across the outer membrane
journal, March 1998
- Thanassi, D. G.; Saulino, E. T.; Lombardo, M. -J.
- Proceedings of the National Academy of Sciences, Vol. 95, Issue 6
Protein Insertion into the Mitochondrial Inner Membrane by a Twin-Pore Translocase
journal, March 2003
- Rehling, P.
- Science, Vol. 299, Issue 5613
Addition of a photocrosslinking amino acid to the genetic code of Escherichia coli
journal, August 2002
- Chin, J. W.; Martin, A. B.; King, D. S.
- Proceedings of the National Academy of Sciences, Vol. 99, Issue 17, p. 11020-11024
The Outer Membrane Usher Guarantees the Formation of Functional Pili by Selectively Catalyzing Donor-Strand Exchange between Subunits That Are Adjacent in the Mature Pilus
journal, February 2010
- Nishiyama, Mireille; Glockshuber, Rudi
- Journal of Molecular Biology, Vol. 396, Issue 1
Analysis of the requirements for pilus biogenesis at the outer membrane usher and the function of the usher C-terminus
journal, April 2006
- Shu Kin So, Stephane; Thanassi, David G.
- Molecular Microbiology, Vol. 60, Issue 2
Stoichiometry of SecYEG in the active translocase of Escherichia coli varies with precursor species
journal, July 2013
- Mao, C.; Cheadle, C. E.; Hardy, S. J. S.
- Proceedings of the National Academy of Sciences, Vol. 110, Issue 29
Crystal structure of the ternary FimC-FimF t -FimD N complex indicates conserved pilus chaperone-subunit complex recognition by the usher FimD
journal, January 2008
- Eidam, Oliv; Dworkowski, Florian S. N.; Glockshuber, Rudi
- FEBS Letters, Vol. 582, Issue 5
Allosteric mechanism of water-channel gating by Ca2+–calmodulin
journal, July 2013
- Reichow, Steve L.; Clemens, Daniel M.; Freites, J. Alfredo
- Nature Structural & Molecular Biology, Vol. 20, Issue 9
Adhesive organelles of Gram-negative pathogens assembled with the classical chaperone/usher machinery: structure and function from a clinical standpoint
journal, May 2010
- Zav'yalov, Vladimir; Zavialov, Anton; Zav'yalova, Galina
- FEMS Microbiology Reviews, Vol. 34, Issue 3
Structural Basis of Chaperone Function and Pilus Biogenesis
journal, August 1999
- Sauer, F. G.
- Science, Vol. 285, Issue 5430
Evolution of the Chaperone/Usher Assembly Pathway: Fimbrial Classification Goes Greek
journal, December 2007
- Nuccio, S. -P.; Baumler, A. J.
- Microbiology and Molecular Biology Reviews, Vol. 71, Issue 4
Recognition of the N-terminal lectin domain of FimH adhesin by the usher FimD is required for type 1 pilus biogenesis: Function of N-domain of FimH in pilus biogenesis
journal, February 2007
- Munera, Diana; Hultgren, Scott; Fernández, Luis Ángel
- Molecular Microbiology, Vol. 64, Issue 2
InsPecT: Identification of Posttranslationally Modified Peptides from Tandem Mass Spectra
journal, July 2005
- Tanner, Stephen; Shu, Hongjun; Frank, Ari
- Analytical Chemistry, Vol. 77, Issue 14
Site-directed, Ligase-Independent Mutagenesis (SLIM) for highly efficient mutagenesis of plasmids greater than 8kb
journal, May 2008
- Chiu, Joyce; Tillett, Daniel; Dawes, Ian W.
- Journal of Microbiological Methods, Vol. 73, Issue 2
Protein secretion in the absence of ATP: the autotransporter, two-partner secretion and chaperone/usher pathways of Gram-negative bacteria (Review)
journal, January 2005
- Thanassi, David G.; Stathopoulos, Christos; Karkal, Aarthi
- Molecular Membrane Biology, Vol. 22, Issue 1-2
X-ray Structure of the FimC-FimH Chaperone-Adhesin Complex from Uropathogenic Escherichia coli
journal, August 1999
- Choudhury, D.
- Science, Vol. 285, Issue 5430
The PapC usher forms an oligomeric channel: Implications for pilus biogenesis across the outer membrane
journal, March 1998
- Thanassi, D. G.; Saulino, E. T.; Lombardo, M. -J.
- Proceedings of the National Academy of Sciences, Vol. 95, Issue 6
The Usher N Terminus Is the Initial Targeting Site for Chaperone-Subunit Complexes and Participates in Subsequent Pilus Biogenesis Events
journal, August 2004
- Ng, T. W.; Akman, L.; Osisami, M.
- Journal of Bacteriology, Vol. 186, Issue 16
An Enhanced System for Unnatural Amino Acid Mutagenesis in E. coli
journal, January 2010
- Young, Travis S.; Ahmad, Insha; Yin, Jun A.
- Journal of Molecular Biology, Vol. 395, Issue 2
The bacterial Sec-translocase: structure and mechanism
journal, April 2012
- Lycklama a. Nijeholt, Jelger A.; Driessen, Arnold J. M.
- Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 367, Issue 1592
Tom40, the Pore-Forming Component of the Protein-Conducting Tom Channel in the Outer Membrane of Mitochondria
journal, June 2001
- Ahting, Uwe; Thieffry, Michel; Engelhardt, Harald
- Journal of Cell Biology, Vol. 153, Issue 6
Insights into pilus assembly and secretion from the structure and functional characterization of usher PapC
journal, April 2009
- Huang, Y.; Smith, B. S.; Chen, L. X.
- Proceedings of the National Academy of Sciences, Vol. 106, Issue 18
Domain activities of PapC usher reveal the mechanism of action of an Escherichia coli molecular machine
journal, May 2012
- Volkan, E.; Ford, B. A.; Pinkner, J. S.
- Proceedings of the National Academy of Sciences, Vol. 109, Issue 24
Works referencing / citing this record:
Handover mechanism of the growing pilus by the bacterial outer-membrane usher FimD
journal, October 2018
- Du, Minge; Yuan, Zuanning; Yu, Hongjun
- Nature, Vol. 562, Issue 7727
UroPathogenic Escherichia coli (UPEC) Infections: Virulence Factors, Bladder Responses, Antibiotic, and Non-antibiotic Antimicrobial Strategies
journal, August 2017
- Terlizzi, Maria E.; Gribaudo, Giorgio; Maffei, Massimo E.
- Frontiers in Microbiology, Vol. 8
Nitazoxanide Inhibits Pilus Biogenesis by Interfering with Folding of the Usher Protein in the Outer Membrane
journal, January 2016
- Chahales, Peter; Hoffman, Paul S.; Thanassi, David G.
- Antimicrobial Agents and Chemotherapy, Vol. 60, Issue 4
Electrostatic networks control plug stabilization in the PapC usher
text, January 2020
- Pham, Thieng; Henderson, Nadine S.; Werneburg, Glenn T.
- Taylor & Francis
Electrostatic networks control plug stabilization in the PapC usher
journal, November 2015
- Pham, Thieng; Henderson, Nadine S.; Werneburg, Glenn T.
- Molecular Membrane Biology, Vol. 32, Issue 5-8
Structural basis for usher activation and intramolecular subunit transfer in P pilus biogenesis in Escherichia coli
journal, October 2018
- Omattage, Natalie S.; Deng, Zengqin; Pinkner, Jerome S.
- Nature Microbiology, Vol. 3, Issue 12
A comprehensive guide to pilus biogenesis in Gram-negative bacteria
journal, May 2017
- Hospenthal, Manuela K.; Costa, Tiago R. D.; Waksman, Gabriel
- Nature Reviews Microbiology, Vol. 15, Issue 6
Electrostatic networks control plug stabilization in the PapC usher
text, January 2020
- Pham, Thieng; Henderson, Nadine S.; Werneburg, Glenn T.
- Taylor & Francis
Electrostatic networks control plug stabilization in the PapC usher
text, January 2016
- Pham, Thieng; Henderson, Nadine S.; Werneburg, Glenn T.
- Taylor & Francis
UroPathogenic Escherichia coli (UPEC) Infections: Virulence Factors, Bladder Responses, Antibiotic, and Non-antibiotic Antimicrobial Strategies
journal, August 2017
- Terlizzi, Maria E.; Gribaudo, Giorgio; Maffei, Massimo E.
- Frontiers in Microbiology, Vol. 8
Structural basis for usher activation and intramolecular subunit transfer in P pilus biogenesis in Escherichia coli
journal, October 2018
- Omattage, Natalie S.; Deng, Zengqin; Pinkner, Jerome S.
- Nature Microbiology, Vol. 3, Issue 12