Search Menu
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information
Search Scholarly Publications

Title: Protein arginine deiminase 2 binds calcium in an ordered fashion: Implications for inhibitor design

Abstract

Protein arginine deiminases (PADs) are calcium-dependent histone-modifying enzymes whose activity is dysregulated in inflammatory diseases and cancer. PAD2 functions as an Estrogen Receptor (ER) coactivator in breast cancer cells via the citrullination of histone tail arginine residues at ER binding sites. Although an attractive therapeutic target, the mechanisms that regulate PAD2 activity are largely unknown, especially the detailed role of how calcium facilitates enzyme activation. To gain insights into these regulatory processes, we determined the first structures of PAD2 (27 in total), and through calcium-titrations by X-ray crystallography, determined the order of binding and affinity for the six calcium ions that bind and activate this enzyme. These structures also identified several PAD2 regulatory elements, including a calcium switch that controls proper positioning of the catalytic cysteine residue, and a novel active site shielding mechanism. Additional biochemical and mass-spectrometry-based hydrogen/deuterium exchange studies support these structural findings. The identification of multiple intermediate calcium-bound structures along the PAD2 activation pathway provides critical insights that will aid the development of allosteric inhibitors targeting the PADs.

Authors:
 [1];  [2];  [3];  [4];  [2];  [4];  [5];  [6];  [5];  [2];  [4];  [3]
+ Show Author Affiliations
  1. The Scripps Research Institute, Jupiter, FL (United States); Virginia Polytechnic Institute and State Univ., Blacksburg, VA (United States)
  2. The Scripps Research Institute, Jupiter, FL (United States)
  3. The Scripps Research Institute, Jupiter, FL (United States); Univ. of Massachusetts Medical School, Worcester, MA (United States)
  4. Washington Univ. in St. Louis, St. Louis, MO (United States)
  5. GlaxoSmithKline (United Kingdom). Medicines Research Centre.
  6. Cornell Univ., Ithaca, NY (United States)
Publication Date:
Research Org.:
SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1215427
Alternate Identifier(s):
OSTI ID: 1215445
Grant/Contract Number:  
W871XWH-07-0372; GM079357; GM103422; AC02-06CH11357
Resource Type:
Accepted Manuscript
Journal Name:
ACS Chemical Biology
Additional Journal Information:
Journal Volume: 10; Journal Issue: 4; Journal ID: ISSN 1554-8929
Publisher:
American Chemical Society
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Slade, Daniel J., Fang, Pengfei, Dreyton, Christina J., Zhang, Ying, Fuhrmann, Jakob, Rempel, Don, Bax, Benjamin D., Coonrod, Scott A., Lewis, Huw D., Guo, Min, Gross, Michael L., and Thompson, Paul R. Protein arginine deiminase 2 binds calcium in an ordered fashion: Implications for inhibitor design. United States: N. p., 2015. Web. doi:10.1021/cb500933j.
Copy to clipboard
Slade, Daniel J., Fang, Pengfei, Dreyton, Christina J., Zhang, Ying, Fuhrmann, Jakob, Rempel, Don, Bax, Benjamin D., Coonrod, Scott A., Lewis, Huw D., Guo, Min, Gross, Michael L., & Thompson, Paul R. Protein arginine deiminase 2 binds calcium in an ordered fashion: Implications for inhibitor design. United States. https://doi.org/10.1021/cb500933j
Copy to clipboard
Slade, Daniel J., Fang, Pengfei, Dreyton, Christina J., Zhang, Ying, Fuhrmann, Jakob, Rempel, Don, Bax, Benjamin D., Coonrod, Scott A., Lewis, Huw D., Guo, Min, Gross, Michael L., and Thompson, Paul R. Mon . "Protein arginine deiminase 2 binds calcium in an ordered fashion: Implications for inhibitor design". United States. https://doi.org/10.1021/cb500933j. https://www.osti.gov/servlets/purl/1215427.
Copy to clipboard
@article{osti_1215427,
title = {Protein arginine deiminase 2 binds calcium in an ordered fashion: Implications for inhibitor design},
author = {Slade, Daniel J. and Fang, Pengfei and Dreyton, Christina J. and Zhang, Ying and Fuhrmann, Jakob and Rempel, Don and Bax, Benjamin D. and Coonrod, Scott A. and Lewis, Huw D. and Guo, Min and Gross, Michael L. and Thompson, Paul R.},
abstractNote = {Protein arginine deiminases (PADs) are calcium-dependent histone-modifying enzymes whose activity is dysregulated in inflammatory diseases and cancer. PAD2 functions as an Estrogen Receptor (ER) coactivator in breast cancer cells via the citrullination of histone tail arginine residues at ER binding sites. Although an attractive therapeutic target, the mechanisms that regulate PAD2 activity are largely unknown, especially the detailed role of how calcium facilitates enzyme activation. To gain insights into these regulatory processes, we determined the first structures of PAD2 (27 in total), and through calcium-titrations by X-ray crystallography, determined the order of binding and affinity for the six calcium ions that bind and activate this enzyme. These structures also identified several PAD2 regulatory elements, including a calcium switch that controls proper positioning of the catalytic cysteine residue, and a novel active site shielding mechanism. Additional biochemical and mass-spectrometry-based hydrogen/deuterium exchange studies support these structural findings. The identification of multiple intermediate calcium-bound structures along the PAD2 activation pathway provides critical insights that will aid the development of allosteric inhibitors targeting the PADs.},
doi = {10.1021/cb500933j},
journal = {ACS Chemical Biology},
number = 4,
volume = 10,
place = {United States},
year = {Mon Jan 26 00:00:00 EST 2015},
month = {Mon Jan 26 00:00:00 EST 2015}
}
Copy to clipboard
Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1021/cb500933j
Copyright Statement
Other availability
Search WorldCat Search WorldCat to find libraries that may hold this journal
Citation Metrics:
Cited by: 80 works
Citation information provided by
Web of Science
Save / Share:
Export Metadata
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.

Works referenced in this record:

PAD, a growing family of citrullinating enzymes: genes, features and involvement in disease
journal, November 2003

  • Vossenaar, Erik R.; Zendman, Albert J. W.; van Venrooij, Walther J.
  • BioEssays, Vol. 25, Issue 11
  • DOI: 10.1002/bies.10357

Citrullination regulates pluripotency and histone H1 binding to chromatin
journal, January 2014

  • Christophorou, Maria A.; Castelo-Branco, Gonçalo; Halley-Stott, Richard P.
  • Nature, Vol. 507, Issue 7490
  • DOI: 10.1038/nature12942

Citrullination unravels stem cells
journal, April 2014

  • Slade, Daniel J.; Subramanian, Venkataraman; Thompson, Paul R.
  • Nature Chemical Biology, Vol. 10, Issue 5
  • DOI: 10.1038/nchembio.1504

Structural basis for Ca2+-induced activation of human PAD4
journal, July 2004

  • Arita, Kyouhei; Hashimoto, Hiroshi; Shimizu, Toshiyuki
  • Nature Structural & Molecular Biology, Vol. 11, Issue 8
  • DOI: 10.1038/nsmb799

Deimination of linker histones links neutrophil extracellular trap release with autoantibodies in systemic autoimmunity
journal, March 2014

  • Dwivedi, Nishant; Neeli, Indira; Schall, Nicolas
  • The FASEB Journal, Vol. 28, Issue 7
  • DOI: 10.1096/fj.13-247254

Peptidylarginine deiminase: a candidate factor in demyelinating disease: A candidate factor in demyelinating disease
journal, April 2002

Peptidylarginine deiminase 2-catalyzed histone H3 arginine 26 citrullination facilitates estrogen receptor   target gene activation
journal, August 2012

  • Zhang, X.; Bolt, M.; Guertin, M. J.
  • Proceedings of the National Academy of Sciences, Vol. 109, Issue 33
  • DOI: 10.1073/pnas.1203280109

Dysregulation of PAD4-mediated citrullination of nuclear GSK3  activates TGF-  signaling and induces epithelial-to-mesenchymal transition in breast cancer cells
journal, July 2013

  • Stadler, S. C.; Vincent, C. T.; Fedorov, V. D.
  • Proceedings of the National Academy of Sciences, Vol. 110, Issue 29
  • DOI: 10.1073/pnas.1308362110

Citrulline is an essential constituent of antigenic determinants recognized by rheumatoid arthritis-specific autoantibodies.
journal, January 1998

  • Schellekens, G. A.; de Jong, B. A.; van den Hoogen, F. H.
  • Journal of Clinical Investigation, Vol. 101, Issue 1
  • DOI: 10.1172/JCI1316

Histone hypercitrullination mediates chromatin decondensation and neutrophil extracellular trap formation
journal, January 2009

  • Wang, Yanming; Li, Ming; Stadler, Sonja
  • Journal of Cell Biology, Vol. 184, Issue 2
  • DOI: 10.1083/jcb.200806072

Identification of Macrophage Extracellular Trap-Like Structures in Mammary Gland Adipose Tissue: A Preliminary Study
journal, January 2013

The protein arginine deiminases: Structure, function, inhibition, and disease
journal, November 2012

  • Bicker, Kevin L.; Thompson, Paul R.
  • Biopolymers, Vol. 99, Issue 2
  • DOI: 10.1002/bip.22127

Suppression of colitis in mice by Cl-amidine: a novel peptidylarginine deiminase inhibitor
journal, June 2011

  • Chumanevich, Alexander A.; Causey, Corey P.; Knuckley, Bryan A.
  • American Journal of Physiology-Gastrointestinal and Liver Physiology, Vol. 300, Issue 6
  • DOI: 10.1152/ajpgi.00435.2010

Peptidylarginine deiminase inhibition is immunomodulatory and vasculoprotective in murine lupus
journal, June 2013

  • Knight, Jason S.; Zhao, Wenpu; Luo, Wei
  • Journal of Clinical Investigation, Vol. 123, Issue 7
  • DOI: 10.1172/JCI67390

Protein deiminases: New players in the developmentally regulated loss of neural regenerative ability
journal, July 2011

Identification of PADI2 as a potential breast cancer biomarker and therapeutic target
journal, October 2012

N-α-Benzoyl-N5-(2-Chloro-1-Iminoethyl)-l-Ornithine Amide, a Protein Arginine Deiminase Inhibitor, Reduces the Severity of Murine Collagen-Induced Arthritis
journal, February 2011

  • Willis, Van C.; Gizinski, Alison M.; Banda, Nirmal K.
  • The Journal of Immunology, Vol. 186, Issue 7
  • DOI: 10.4049/jimmunol.1001620

Peptidylarginine Deiminase Inhibition Reduces Vascular Damage and Modulates Innate Immune Responses in Murine Models of Atherosclerosis
journal, March 2014

Peptidylarginine deiminase inhibition disrupts NET formation and protects against kidney, skin and vascular disease in lupus-prone MRL/ lpr mice
journal, August 2014

  • Knight, Jason S.; Subramanian, Venkataraman; O'Dell, Alexander A.
  • Annals of the Rheumatic Diseases, Vol. 74, Issue 12
  • DOI: 10.1136/annrheumdis-2014-205365

Genomic Index of Sensitivity to Endocrine Therapy for Breast Cancer
journal, September 2010

  • Symmans, W. Fraser; Hatzis, Christos; Sotiriou, Christos
  • Journal of Clinical Oncology, Vol. 28, Issue 27
  • DOI: 10.1200/JCO.2010.28.4273

Identification and validation of an ERBB2 gene expression signature in breast cancers
journal, January 2004

  • Bertucci, François; Borie, Nathalie; Ginestier, Christophe
  • Oncogene, Vol. 23, Issue 14
  • DOI: 10.1038/sj.onc.1207361

Inhibition of PAD4 activity is sufficient to disrupt mouse and human NET formation
journal, January 2015

  • Lewis, Huw D.; Liddle, John; Coote, Jim E.
  • Nature Chemical Biology, Vol. 11, Issue 3
  • DOI: 10.1038/nchembio.1735

Data mining of metal ion environments present in protein structures
journal, September 2008

Erosive Rheumatoid Arthritis Is Associated with Antibodies That Activate PAD4 by Increasing Calcium Sensitivity
journal, May 2013

Microdomains of high calcium concentration in a presynaptic terminal
journal, May 1992

Calcium Green FlAsH as a genetically targeted small-molecule calcium indicator
journal, June 2007

  • Tour, Oded; Adams, Stephen R.; Kerr, Rex A.
  • Nature Chemical Biology, Vol. 3, Issue 7
  • DOI: 10.1038/nchembio.2007.4

[20] Processing of X-ray diffraction data collected in oscillation mode
book, January 1997

PHENIX: a comprehensive Python-based system for macromolecular structure solution
journal, January 2010

  • Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor
  • Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2, p. 213-221
  • DOI: 10.1107/S0907444909052925

Coot model-building tools for molecular graphics
journal, November 2004

  • Emsley, Paul; Cowtan, Kevin
  • Acta Crystallographica Section D Biological Crystallography, Vol. 60, Issue 12, p. 2126-2132
  • DOI: 10.1107/S0907444904019158

Insights into rheumatoid arthritis derived from the Sa immune system
journal, January 2000

  • Ménard, Henri A.; Lapointe, Elvy; Rochdi, Moulay D.
  • Arthritis Research, Vol. 2, Issue 6, p. 429
  • DOI: 10.1186/ar122

UCSF Chimera?A visualization system for exploratory research and analysis
journal, January 2004

  • Pettersen, Eric F.; Goddard, Thomas D.; Huang, Conrad C.
  • Journal of Computational Chemistry, Vol. 25, Issue 13
  • DOI: 10.1002/jcc.20084

LigPlot+: Multiple Ligand–Protein Interaction Diagrams for Drug Discovery
journal, October 2011

  • Laskowski, Roman A.; Swindells, Mark B.
  • Journal of Chemical Information and Modeling, Vol. 51, Issue 10
  • DOI: 10.1021/ci200227u

Kinetic Characterization of Protein Arginine Deiminase 4:  A Transcriptional Corepressor Implicated in the Onset and Progression of Rheumatoid Arthritis †
journal, August 2005

  • Kearney, Patricia L.; Bhatia, Monica; Jones, Nelroy G.
  • Biochemistry, Vol. 44, Issue 31
  • DOI: 10.1021/bi050292m

A Colorimetric 96-Well Microtiter Plate Assay for the Determination of Enzymatically Formed Citrulline
journal, November 2000

Native Electrospray and Electron-Capture Dissociation FTICR Mass Spectrometry for Top-Down Studies of Protein Assemblies
journal, July 2011

  • Zhang, Hao; Cui, Weidong; Wen, Jianzhong
  • Analytical Chemistry, Vol. 83, Issue 14
  • DOI: 10.1021/ac200695d

AutoDock4 and AutoDockTools4: Automated docking with selective receptor flexibility
journal, December 2009

  • Morris, Garrett M.; Huey, Ruth; Lindstrom, William
  • Journal of Computational Chemistry, Vol. 30, Issue 16
  • DOI: 10.1002/jcc.21256

The protein arginine deiminases: Structure, function, inhibition, and disease
journal, November 2012

  • Bicker, Kevin L.; Thompson, Paul R.
  • Biopolymers, Vol. 99, Issue 2
  • DOI: 10.1002/bip.22127

UCSF Chimera?A visualization system for exploratory research and analysis
journal, January 2004

  • Pettersen, Eric F.; Goddard, Thomas D.; Huang, Conrad C.
  • Journal of Computational Chemistry, Vol. 25, Issue 13
  • DOI: 10.1002/jcc.20084

AutoDock4 and AutoDockTools4: Automated docking with selective receptor flexibility
journal, December 2009

  • Morris, Garrett M.; Huey, Ruth; Lindstrom, William
  • Journal of Computational Chemistry, Vol. 30, Issue 16
  • DOI: 10.1002/jcc.21256

A Colorimetric 96-Well Microtiter Plate Assay for the Determination of Enzymatically Formed Citrulline
journal, November 2000

Inducement of ER Stress by PAD Inhibitor BB-Cl-Amidine to Effectively Kill AML Cells
journal, October 2022

Protein deiminases: New players in the developmentally regulated loss of neural regenerative ability
journal, July 2011

Native Electrospray and Electron-Capture Dissociation FTICR Mass Spectrometry for Top-Down Studies of Protein Assemblies
journal, July 2011

  • Zhang, Hao; Cui, Weidong; Wen, Jianzhong
  • Analytical Chemistry, Vol. 83, Issue 14
  • DOI: 10.1021/ac200695d

Kinetic Characterization of Protein Arginine Deiminase 4:  A Transcriptional Corepressor Implicated in the Onset and Progression of Rheumatoid Arthritis †
journal, August 2005

  • Kearney, Patricia L.; Bhatia, Monica; Jones, Nelroy G.
  • Biochemistry, Vol. 44, Issue 31
  • DOI: 10.1021/bi050292m

Citrullination unravels stem cells
journal, April 2014

  • Slade, Daniel J.; Subramanian, Venkataraman; Thompson, Paul R.
  • Nature Chemical Biology, Vol. 10, Issue 5
  • DOI: 10.1038/nchembio.1504

Inhibition of PAD4 activity is sufficient to disrupt mouse and human NET formation
journal, January 2015

  • Lewis, Huw D.; Liddle, John; Coote, Jim E.
  • Nature Chemical Biology, Vol. 11, Issue 3
  • DOI: 10.1038/nchembio.1735

Calcium Green FlAsH as a genetically targeted small-molecule calcium indicator
journal, June 2007

  • Tour, Oded; Adams, Stephen R.; Kerr, Rex A.
  • Nature Chemical Biology, Vol. 3, Issue 7
  • DOI: 10.1038/nchembio.2007.4

Identification and validation of an ERBB2 gene expression signature in breast cancers
journal, January 2004

  • Bertucci, François; Borie, Nathalie; Ginestier, Christophe
  • Oncogene, Vol. 23, Issue 14
  • DOI: 10.1038/sj.onc.1207361

Peptidylarginine deiminase 2-catalyzed histone H3 arginine 26 citrullination facilitates estrogen receptor   target gene activation
journal, August 2012

  • Zhang, X.; Bolt, M.; Guertin, M. J.
  • Proceedings of the National Academy of Sciences, Vol. 109, Issue 33
  • DOI: 10.1073/pnas.1203280109

Histone hypercitrullination mediates chromatin decondensation and neutrophil extracellular trap formation
journal, January 2009

  • Wang, Yanming; Li, Ming; Stadler, Sonja
  • Journal of Cell Biology, Vol. 184, Issue 2
  • DOI: 10.1083/jcb.200806072

Deimination of linker histones links neutrophil extracellular trap release with autoantibodies in systemic autoimmunity
journal, March 2014

  • Dwivedi, Nishant; Neeli, Indira; Schall, Nicolas
  • The FASEB Journal, Vol. 28, Issue 7
  • DOI: 10.1096/fj.13-247254

The geometry of metal–ligand interactions relevant to proteins. II. Angles at the metal atom, additional weak metal–donor interactions
journal, July 2000

  • Harding, Marjorie M.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 56, Issue 7
  • DOI: 10.1107/s0907444900005849

Suppression of colitis in mice by Cl-amidine: a novel peptidylarginine deiminase inhibitor
journal, June 2011

  • Chumanevich, Alexander A.; Causey, Corey P.; Knuckley, Bryan A.
  • American Journal of Physiology-Gastrointestinal and Liver Physiology, Vol. 300, Issue 6
  • DOI: 10.1152/ajpgi.00435.2010

Neutrophils Cast NETs in Atherosclerosis
journal, March 2014

Is It Time to ReSET the Standard for Estrogen Receptor Testing in Breast Cancer?
journal, September 2010

  • Oesterreich, Steffi; Lee, Adrian V.; Davidson, Nancy E.
  • Journal of Clinical Oncology, Vol. 28, Issue 27
  • DOI: 10.1200/jco.2010.30.9864

PHENIX: a comprehensive Python-based system for macromolecular structure solution.
text, January 2010

  • Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor
  • Apollo - University of Cambridge Repository
  • DOI: 10.17863/cam.45787

Identification of Macrophage Extracellular Trap-Like Structures in Mammary Gland Adipose Tissue: A Preliminary Study
journal, January 2013

N-α-Benzoyl-N5-(2-Chloro-1-Iminoethyl)-l-Ornithine Amide, a Protein Arginine Deiminase Inhibitor, Reduces the Severity of Murine Collagen-Induced Arthritis
journal, February 2011

  • Willis, Van C.; Gizinski, Alison M.; Banda, Nirmal K.
  • The Journal of Immunology, Vol. 186, Issue 7
  • DOI: 10.4049/jimmunol.1001620
    Sort by:
    [ × clear filter / sort ]

    Works referencing / citing this record:

    Deimination and Peptidylarginine Deiminases in Skin Physiology and Diseases
    journal, January 2020

    • Méchin, Marie-Claire; Takahara, Hidenari; Simon, Michel
    • International Journal of Molecular Sciences, Vol. 21, Issue 2
    • DOI: 10.3390/ijms21020566

    In vivo expression of peptidylarginine deiminase in Drosophila melanogaster
    journal, January 2020

    Structure and mechanism of a bacterial host-protein citrullinating virulence factor, Porphyromonas gingivalis peptidylarginine deiminase
    journal, July 2015

    • Goulas, Theodoros; Mizgalska, Danuta; Garcia-Ferrer, Irene
    • Scientific Reports, Vol. 5, Issue 1
    • DOI: 10.1038/srep11969

    Autoantibodies to protein-arginine deiminase (PAD) 4 in rheumatoid arthritis: immunological and clinical significance, and potential for precision medicine: Anti-PAD4 antibodies in RA
    journal, October 2019

    • Martinez-Prat, Laura; Palterer, Boaz; Vitiello, Gianfranco
    • Expert Review of Clinical Immunology, Vol. 15, Issue 10
    • DOI: 10.1080/1744666x.2020.1668778

    Phosphorylation-dependent protein design: design of a minimal protein kinase-inducible domain
    journal, January 2019

    • Gao, Feng; Thornley, Blair S.; Tressler, Caitlin M.
    • Organic & Biomolecular Chemistry, Vol. 17, Issue 16
    • DOI: 10.1039/c9ob00502a

    Affinity maturation shapes the function of agonistic antibodies to peptidylarginine deiminase type 4 in rheumatoid arthritis
    journal, October 2017

    Protein-arginine deiminase 2 suppresses proliferation of colon cancer cells through protein citrullination
    journal, April 2017

    • Funayama, Ryo; Taniguchi, Hajime; Mizuma, Masamichi
    • Cancer Science, Vol. 108, Issue 4
    • DOI: 10.1111/cas.13179

    Rheumatoid Arthritis-Associated Mechanisms of Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans
    journal, August 2019

    • Gómez-Bañuelos, Eduardo; Mukherjee, Amarshi; Darrah, Erika
    • Journal of Clinical Medicine, Vol. 8, Issue 9
    • DOI: 10.3390/jcm8091309

    Halogen Bonding Increases the Potency and Isozyme Selectivity of Protein Arginine Deiminase 1 Inhibitors
    journal, July 2019

    • Mondal, Santanu; Gong, Xuefeng; Zhang, Xiaoqian
    • Angewandte Chemie, Vol. 131, Issue 36
    • DOI: 10.1002/ange.201906334

    Exceptionally versatile – arginine in bacterial post-translational protein modifications
    journal, November 2019

    • Lassak, Jürgen; Koller, Franziska; Krafczyk, Ralph
    • Biological Chemistry, Vol. 400, Issue 11
    • DOI: 10.1515/hsz-2019-0182

    GnRH Stimulates Peptidylarginine Deiminase Catalyzed Histone Citrullination in Gonadotrope Cells
    journal, September 2016

    • Khan, Shaihla A.; Edwards, Brian S.; Muth, Aaron
    • Molecular Endocrinology, Vol. 30, Issue 10
    • DOI: 10.1210/me.2016-1085

    Crystal structure of Porphyromonas gingivalis peptidylarginine deiminase: implications for autoimmunity in rheumatoid arthritis
    journal, July 2015

    Small molecule promotes β-catenin citrullination and inhibits Wnt signaling in cancer
    journal, October 2017

    • Qu, Yi; Olsen, Jan Roger; Yuan, Xing
    • Nature Chemical Biology, Vol. 14, Issue 1
    • DOI: 10.1038/nchembio.2510

    Halogen Bonding Increases the Potency and Isozyme Selectivity of Protein Arginine Deiminase 1 Inhibitors
    journal, September 2019

    • Mondal, Santanu; Gong, Xuefeng; Zhang, Xiaoqian
    • Angewandte Chemie International Edition, Vol. 58, Issue 36
    • DOI: 10.1002/anie.201906334

    Structure, function, and inhibition of a genomic/clinical variant of Porphyromonas gingivalis peptidylarginine deiminase: Structure and Function of PPAD-T2
    journal, January 2019

    • Bereta, Grzegorz; Goulas, Theodoros; Madej, Mariusz
    • Protein Science, Vol. 28, Issue 3
    • DOI: 10.1002/pro.3571

    An interplay of structure and intrinsic disorder in the functionality of peptidylarginine deiminases, a family of key autoimmunity-related enzymes
    journal, July 2019

    • Alghamdi, Mohammed; Al Ghamdi, Khaled A.; Khan, Rizwan H.
    • Cellular and Molecular Life Sciences, Vol. 76, Issue 23
    • DOI: 10.1007/s00018-019-03237-8

    Structure and mechanism of a bacterial host-protein citrullinating virulence factor, Porphyromonas gingivalis peptidylarginine deiminase.
    text, January 2015

    • Goulas, Theodoros; Mizgalska, Danuta; Garcia-Ferrer, Irene
    • Apollo - University of Cambridge Repository
    • DOI: 10.17863/cam.58789

    The Development of Benzimidazole-Based Clickable Probes for the Efficient Labeling of Cellular Protein Arginine Deiminases (PADs)
    journal, January 2018

    • Nemmara, Venkatesh V.; Subramanian, Venkataraman; Muth, Aaron
    • ACS Chemical Biology, Vol. 13, Issue 3
    • DOI: 10.1021/acschembio.7b00957

    Structure and mechanism of a bacterial host-protein citrullinating virulence factor, Porphyromonas gingivalis peptidylarginine deiminase
    journal, July 2015

    • Goulas, Theodoros; Mizgalska, Danuta; Garcia-Ferrer, Irene
    • Scientific Reports, Vol. 5, Issue 1
    • DOI: 10.1038/srep11969

    Molecular Interplay between the Dimer Interface and the Substrate-Binding Site of Human Peptidylarginine Deiminase 4
    journal, February 2017

    • Lee, Chien-Yun; Lin, Chu-Cheng; Liu, Yi-Liang
    • Scientific Reports, Vol. 7, Issue 1
    • DOI: 10.1038/srep42662

    Crystal structure of Porphyromonas gingivalis peptidylarginine deiminase: implications for autoimmunity in rheumatoid arthritis
    journal, July 2015

    In vivo expression of peptidylarginine deiminase in Drosophila melanogaster
    journal, January 2020

    Thioredoxin Modulates Protein Arginine Deiminase 4 (PAD4)-Catalyzed Citrullination
    journal, February 2019

    Deimination and Peptidylarginine Deiminases in Skin Physiology and Diseases
    journal, January 2020

    • Méchin, Marie-Claire; Takahara, Hidenari; Simon, Michel
    • International Journal of Molecular Sciences, Vol. 21, Issue 2
    • DOI: 10.3390/ijms21020566
      Sort by:
      [ × clear filter / sort ]

      Similar Records in DOE PAGES and OSTI.GOV collections: